Bacterial Toxin Research Citations

… was coated with purified goat F(ab’) 2 anti-mouse IgM + IgG + IgA antibody (200 ng/well in 100 µl of PBS; SouthernBiotech, Birmingham, AL), attenuated cholera toxin (CT) (200 ng/well in 100 µl of PBS; List Biological Laboratories …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

The Chronic EAE Model:

To evaluate AIS integrity in an inflammatory environment, we induced the chronic
model of experimental autoimmune encephalomyelitis (EAE) as previously described
(DeVries et al., 2012; Secor McVoy et al., 2015; Dupree et al., 2015). … Mice were also injected intraperitoneal (i.p.) on the same day with 300 ng Pertussis toxin (PT) (List Biological Labs, Campbell, California) in 200 µL phosphate buffered saline (PBS) with a booster PT injection 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

 … MOG emulsion was transferred to 1 ml Luer-Lok syringes (BD Falcon) with 27G inch needles. Pertussis toxin was diluted in UltraPure (2 ng/l; List Biologicals Laboratories, Campbell, CA) and transferred to syringes as described above. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Given the important role of pH change in the anthrax toxin internalization process, the current study is designed to address the disparity.

Material and Methods:

PA63 was purchased from List Biological Laboratories, Inc. (Campbell, CA). …

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Surgery:

… For VP cell lesions or retrograde NAc labelling, mice were unilaterally injected into the VP (0.4 mm anterior and 1.3 mm lateral to bregma, 4.3 mm below the dura) with 5% ibotenic acid (30 nl per injection; Tocris, 0285) or 0.2% CTb (18 nl per injection; List Biological, Cat# 104), respectively. …

Histology:

Immunohistochemistry was performed on free-floating sections as described previously¹⁴. In brief, sections were rinsed in PBS, incubated in 0.3% hydrogen peroxide in PBS for 30min at room temperature, and then sequentially at room temperature in 3% normal donkey serum and 0.25% Triton X-100 in PBS (PBT) for 1h and primary antibody diluted in PBT with 0.02% sodium azide overnight. After overnight incubation with … or goat anti-CTb antibody (1:15,000; List Biological, Cat# 703), sections were rinsed and incubated for 2h in biotinylated antibody …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… For the experiments involving the Gi inhibitor, PTX (List Biological Laboratories Inc., Campbell, CA) and 8-Br-cAMP (Sigma-Aldrich), cells were pre-treated with the two compounds for 30 min …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… For EAE induction incomplete Freund’s adjuvant (IFA) and killed Mycobacterium tuberculosis strain H37RA were purchased from Difco (Sparks, MD), and pertussis toxin was purchased from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera Toxin (100 ng/mL) (List Biological Laboratories, Campbell, CA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE:

… 200 ng of pertussis toxin (List Biological Lab, Epsom, England) was given intraperitoneally on days 0 and 2 post-immunization (pi). EAE was assessed by a 0-5 scoring system described previously (Stromnes and Goverman, 2006). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

– … solution; #103, List Biological Laboratories, INC, Campbell, CA) or Fluorogold (2.5-5.0% FG, … They were then incubated in the goat anti-CTB antiserum (List Biological Cat# 703 RRID: AB_2313637) at 1:2,000 for 2 days at 4°C. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)