Bacterial Toxin Research Citations

Cell Depletion:

KPCD mice received one intraperitoneal injection of DT (25ng/g body weight; List Biological Laboratories) or matched volume of PBS. Monocyte depletion was analysed 24h later by flow cytometry.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Motor and sensory neurons were retrogradely labeled in vivo at p14p16 by intramuscular injection of 1% unconjugated Cholera toxin B subunit (CTB, List Biologicals). Animals were analyzed after 57 days.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Chemicals and Reagents:

… Recombinant anthrax lethal factor (LF) from B. anthracis was from List Biological Laboratories (Campbell, California).

Characterisation of the thresholds for LF and EF detection:

In a first step, we characterised the threshold for the enzymatic quantification of LF and EF by using plasma and ear tissue samples derived from mice infected…

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

Induction of EAU and treatment:

Each C57BL/6J mouse was immunized subcutaneously in one rear footpad and the tail head, and rump (total of 200l) with an emulsion containing 300µg of human IRBP₁₂₀… Simultaneously, the mice received 1µg of Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA, USA) intraperitoneally. Then the mice were randomly divided into a chrysin-treated group (n=30 mice) and a control group (n=30 mice). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…immunized with 200 µg MOG 3355 peptide emulsified in complete Freund’s adjuvant (Sigma) containing Mycobacterium tuberculosis H37 RA (final concentration 2 mg/ml) on day 0, and ip administered with 200 ng pertussis toxin (List Biological Laboratories)…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mucosal immunization

For intranasal (i.n.) administration, immunization cocktails were made by mixing the indicated proteins with PBS. Each dose (20 μl total, 10 μl per nostril) contained either CTB-MPR (35 μg ≡ 12 μg MPER ≡ 2 nmol MPER) with cholera toxin (CT, 1 μg, List Biological Laboratories), VLP preparation (1.1 mg total protein containing 48 μg dgp41 and 48 μg Gag, equivalent to 2 nmol and 1 nmol, respectively) with either CT (1 μg), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Reagent:  1 nM cholera toxin (List Biological Laboratory, Campbell, CA)

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction and LPS instillation:

Active EAE was induced by immunizing the mice with MOG₃₅₅₅ peptide (100 µg) emulsified in complete Freunds adjuvant (Sigma) containing heat-killed Mycobacterium tuberculosis H37Ra (200 µg per mouse, DIFCO Laboratories) on day 0, and intraperitoneal injection of 200ng pertussis toxin (PTx; List Biological Laboratories) on days 0 and 2. To induce T_h17-mediated passive EAE, CD4⁺ T cells, obtained from 2D2 mice, were polarized with rIL-6 (20ng ml¹), rIL-23 (10ng ml¹), rTGF- (3ng ml¹) and anti-IFN- antibody (4 g ml¹) on a plate coated with anti-CD3 and anti-CD28 antibodies for 5 days and adoptively transferred to sublethally irradiated (450 rads) WT and Atg7 CKO mice. PTx (200ng) was intraperitoneally administrated on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

CTb into NAc shell:

We unilaterally injected 40 nl of 1% CTb (List Biological Laboratories) into the NAc shell over 4 min with the needle left in place for an additional 2 min.

CTb injection site verification:

Immunohistochemical procedures were based on our previously published work…We selected a 1-in-4 series of sections from the NAc shell of each rat, and immunofluorescence was used to determine CTb injection sites. We repeatedly rinsed free-floating sections in PBS (3 10 min washes) and incubated them for 2 h in PBS containing 0.5% Triton X-100 (PBS-tx) with 10% normal horse serum (NHS). We then incubated all sections for at least 48 h at 4°C in goat anti-CTb primary antibody (1:5000, List Biological Laboratories, 703),

Fos protein and CTb double labeling:

We processed a 1-in-4 series of vmPFC, PVT, BLA, and vSub for immunohistochemical detection of Fos protein and CTb…We then incubated sections for at least 48 h at 4°C in PBS-tx with 2% NHS, rabbit anti-c-Fos primary antibody (1:8000, Cell Signaling Technology, Phospho-c-Fos, 5348S) and goat anti-CTb primary antibody (1:5000, List Biological Laboratories, 703).

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE:

… injected subcutaneously into the tail base. Immediately thereafter, they received 200 ng pertussis toxin (List Biological) into the foot pad [21]. The animals were examined daily for signs of EAE. Clinical signs were evaluated as …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)