Bacterial Toxin Research Citations

Experimental design:

Sensitization and challenge of mice were performed as described by Lpez-Expsito et al.17 BALB/c and C3H/HeOuJ mice (5 per group) were sensitized once per week for 6 weeks, by gavage with 2 different doses of OVA-LPS-free (1 and 5 mg) dissolved in 0.5 mL of 0.2 M bicarbonate with 10 µg of cholera toxin (CT) (List Biologicals, Campbell, CA, USA). Nave mice received 10 µg of CT in 0.5 mL of bicarbonate. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials:

…Azide-free CT from V. cholere Inaba 569B was obtained from List Biological Laboratories (Campbell, CA). …

cAMP assay:

Caco-2 cells (7.0 × 105 cells/2 mL medium) were seeded in 6-well plastic plates. One week later, the cells were washed twice with PBS and exposed for 30 min–2.5 mM HEPES buffer containing 0.5 µg/mL of GM1 or in the absence of GM1. Subsequently, the cells were washed twice, and then CT and 1 mM IBMX were added to the cultured cells in 2 mL of 0.01% BSA in 2.5 mM HEPES buffer containing peptide P3 (50 nM/mL or 50 µM) or in the absence of P3. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

MTE cells were isolated from tracheas by using overnight incubation with 0.1% protease 14 (Sigma-Aldrich, St Louis, Mo) and cultured on rat tail collagen I gel (BD Biosciences) in DMEM/F12 media (Invitrogen) supplemented with 20 ng/mL cholera toxin (List Biological Laboratories, Campbell, Calif),…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… In addition, a smooth type LPS from E. coli O55:B5, and Rc type LPS from E. coli J5 (List Biological Laboratories, CA, USA), …

• Product #203 – LPS from Escherichia coli O55:B5
• Product #301 – LPS from Escherichia coli J5 (Rc)

The Chronic EAE Model:

An inflammatory environment was created by inducing mice with the chronic model of experimental autoimmune encephalomyelitis (EAE) as previously described …  On the sameday, mice were injected intraperitoneal (IP) with 300ng Pertussis toxin (List Biological Labs, Campbell,CA) in 200 µL phosphate buffered saline (PBS). A booster IP injection of Pertussis toxin was given 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

For active EAE, C57BL/6 mice were immunized s.c. in both flanks with 100 mg myelin oligodendrocyte glycoprotein (MOG) 3555 peptide… On the day of immunization and 2 d after, mice received 200 ng pertussis toxin (List Biological Laboratories) in 100 ml PBS i.v.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Pertussis toxin (PTX) was obtained from List Biological Laboratories (Campbell, CA, USA).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Mycobacterium tuberculosis (Strain H37, Difco). For active EAE, pertussis toxin (List Biological Laboratories) was injected intravenously at Days 0 and 2 (200 and 400 ng) post-immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

DT and clodronate liposome injections:

DT (lot number 15043A1, diluted in PBS; List Biological Laboratories) was administered via i.p. injection on day 22 (50 mg/kg mouse) and day 21 (15 mg/kg) of the priming period prior to i.t. LPS as described previously (39). Mice harvested on day +5 also received a DT dose (15 mg/kg) on day +2 after i.t. LPS. Clodronate liposomes (Cl2MDP) or PBS liposomes (control) were prepared as previously described (8), followed by o.p. instillation (60 ml) on day 23, and i.p. injection (500 ml) on days 22 and 21 of the priming period.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Retrogrde Tracing:

Retrograde labeling of graft neurons extending axons to caudal spinal cord segments was conducted by injecting 1 µl CTB (1% solution; List Biological Laboratories, Campbell, California) into bilateral C6/7 spinal gray matter in four Fischer 344 rats that had undergone C4-CST lesions and grafts of NPCs into the lesion site. CTB was injected 6 weeks after lesion and grafting, and animals were perfused 3 d later.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt