Bacterial Toxin Research Citations

In Vitro T-Cell Homing Assay:

… pertussis toxin (List Biological) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents: 

 Recombinant full-length protective antigen (PA), lethal factor (LF), edema factor (EF) and 
 fluorescein-conjugated PA were purchased from List Biological Laboratories  (Campbell, CA, 
 U.S.A.).

PA-binding assay:

 To detect PA-binding ability of the cells, cells were washed twice with ice-cold PBS, scraped, and 
 resuspended in ice-cold PBS (4 x 106 cells / ml). Cell suspensions (each 25 l) were reacted with 1
 mg/ml of FITC-PA in 1.5 ml tubes and incubated at 4 C for 60 min in the dark.  The cells were
 then washed twice with 2 ml ice-cold PBS and re-suspended in 0.5 ml ice-cold PBS for
flowcytometric analysis as described above.

RESULTS:

 Sensitivity to ATX-mediated cytotoxicity has been confirmed in several cell types derived from 
 mice and humans.  Murine (Raw264.7 or J774.1) or human (THP1, U937 or K562) cells were 
 treated with 300 ng/ml of LF and with the indicated concentration of PA, and viabilities of the cells 
 were examined 24, 48 or 72 hr after the treatment (Fig. 1A).  In the case of murine cells, ATX
 treatment showed a cytotoxic effect in a PA dose-dependent manner at 24 hr after treatment. 
 Comparing with murine cells, human U937 and K562 cells had exhibited resistance to 
 ATX-mediated cytotoxicity in the presence of a high concentration (500 ng/ml) of PA.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis 

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

EAE Induction:

Pertussis toxin (Cat# 180. 50 µg. List Biological Laboratories, Inc.) was diluted with sterile PBS to 100g/ml.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… encephalomyelitis (EAE). Bordetella pertussis toxin (200 ng, PTX, List Biological Laboratories, Campbell, CA) was administered intra-peritoneally on the same day and 2 days after immunization to enhance the autoimmune response. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Active EAE induction:

Female WT or Cav-1 KO mice were subcutaneously immunized with 200 µg MOG₃₅₅₅ in complete Freund’s adjuvant …Pertussis toxin (200 ng, List Biological Laboratories) was injected intraperitoneally twice on day 0 and 48 h later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE immunization and clinical evaluation:

For active EAE, mice were immunized subcutaneously with 175 µg MOG_35-55 (Anaspec) emulsified in Incompete Freunds Adjuvant (Sigma-Aldrich) supplemented with 0.4 mg Mycobacterium tuberculosis H37 (Difco Laboratories) on day 0 and given 200 ng pertussis toxin (List Biologicals) intraperitoneally (IP) on day 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

…rPA was obtained from List Biological Laboratories, Inc. The assay endpoint is reported as the serum mean concentration of anti-PA specific IgG (g/mL). … viability. rPA and rLF were obtained from List Biological Laboratories, Inc. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Induction and assessment of EAE:

…For disease induction, mice were immunized subcutaneously in both sides of the flank with 200 µg MP4 (stock: 2 mg/ml) in CFA. Control mice remained untreated. On the day of immunization and 48 h later, 200 ng pertussis toxin (PTX; List Biological Laboratories, Hornby, ONT, Canada) were given in 500 µl sterile phosphate-buffered saline (PBS). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

ON Injury

ON crush surgery and intraocular injections were performed under general anesthesia. The mice received either 6.4 μg or 25 μg of zymosan 5 days prior and immediately after ON crush into the vitreous. At 5 days prior to sacrifice, CTB (3 μL, 1%; 103B, List Biological) was injected as an anterograde tracer. For C57BL/6 control n = 5; 6.4 μg n = 4; 25 μg n = 5. For CAST/Ei control n = 4; 6.4 μg n = 4; 25 μg n = 6. Quantification was performed blinded to the treatment and mouse strain.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

DT preparation and administration:

DT (List Biological Laboratories Inc., catalog 150) was administered to NSG-DTR mice in a single, 300 l i.p. injection of 0.5, 1.0, 2.5, 5.0, 10, or 25 ng total DT. Control NSG-DTR mice (PBS) were treated with an equal volume…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae