Bacterial Toxin Research Citations

EAE:

Two hundred nanograms pertussis toxin (List Biological Laboratories, Campbell, CA) was administered iv on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…containing 400 µg killed Mycobacterium tuberculosis (Difco Laboratories, Detroit, MI) on day 0 followed by ip treatments with 200 ng Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

… The animals also received 200 ng pertussis toxin (List Biological Laboratories) ip on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental animals:

… In a separate cohort of Gaa^/ animals (n = 4), intrapleural injection (20 l) of cholera toxin subunit dissolved in sterile saline for a 2% solution (List Biological Laboratories, Campbell, CA) was used to retrogradely label phrenic motoneurons. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE induction

EAE was induced in mice by subcutaneous (S.C.) injection into the hindquarters of 50 µg of myelin oligodendrocyte glycoprotein (MOG₃₅₅₅), …Immunized mice also received two intraperitoneal (IP) injections of pertussis toxin (Bordatella pertussis) (PT, List Biological LabsCampbell, CA, USA)first, on the day of the induction and again 48 h later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

CT was purchased from List Biological Laboratories (Campbell, CA). …

Analysis of lung migratory dendritic cells and BMDCs:

Mice (n = 15) were i.n. administered with 2 µg of CT and medLN cells were prepared before or 13 days after the administration. For i.n. administration, mice were lightly anesthetized by isoflurane (Ifran, Hana Pharm, Kyounggi-Do, Korea) inhalation and CT in a volume of 50 µl of phosphate-buffered saline (PBS) was applied to the left nostril. The CT-administered mice didnt have any pathologic appearance compared to untreated mice during the days. …

Cytokine assays:

BMDCs were incubated with PBS or CT (2 µg/ml) for the indicated days and the culture supernatants were harvested at the indicated time points. …

In vitro T cell differentiation:

To induce T cell differentiation in DC-assisted system, isolated in vivo migratory DC subsets, BMDCs (untreated or CT-treated), or phenotype-based, isolated BMDCs were used. …The BMDCs were incubated with CT for a day and in some experiments, CT-treated BMDCs were FACS sorted using FACSAria II (BD Bioscience) based on CD86 expression. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Reagents:

Lipopolysaccharide (LPS) from Escherichia coli O111:B4 was purchased from List Biological Laboratories, Inc.

• Product #201 – LPS from Escherichia coli O111:B4

PBMC isolation and culture:

PBMC were isolated from collected blood or leukopheresis by density gradient centrifugation, according to the manufacturers instructions. Cells were cryopreserved in liquid nitrogen suspended in FBS containing 10% (vol/vol) DMSO. Culturing of PBMCs for in vitro expansion was performed by incubating in … and Fim2/3 (List Biological Labs) at 5 g/mL. …

• Product #186 – Fimbriae 2/3 from B. pertussis

IL4 gene therapy of EAE mouse model:

Chronic EAE was induced in female C57BL/6 mice, by subcutaneous with 300µl of 200µg per mouse of MOG3555 in Freunds Adjuvant Incomplete liquid, …Pertussis toxin (500 ng, List Biological Laboratories, Campbell, CA, USA) was injected i.v. on the day of the immunization and again 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Model:

C57Bl/6 (10-week-old female) mice were…and immunized subcutaneously with 200 µg MOG₃₅₅₅ (GenScript) mixed 1:1 with supplemented complete Freunds adjuvant followed by 200 ng pertussis toxin (PTX; List Biological Laboratories) intraperitoneally (i.p.) at days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)