Bacterial Toxin Research Citations

Materials:

Pertussis toxin (PTx) from Bordetella pertussis (no. 181), Pertussis toxin subunit (B-oligomer, no. 184), and Diphtheria toxin (DT) from Corynebacterium diphtheriae (no. 150) were purchased from List Biological Laboratories Inc. …

For DT samples, Cd11c-eyfp+dtr+ aortas were used
and the perfusate contained diphtheria toxin (DT) to induce apoptosis. Cells did not
disappear, instead cell debris were continually being shed off and ghost dead cells are
left behind at the 6 hour time point.

WT mice were injected with LPS for 6 hours (white bars) or 12 hours (black
bars). The latter groups were treated with PTx or CCL19 blocking antibody; and
controls were injected with B-oligomer or nonimmune IgG, respectively. Intimal mRNA
expression was analyzed and data were normalized to mice injected with LPS for 6
hours (white bars).

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Experimental autoimmune encephalomyelitis induction:

… EAE was induced in male C57BL/6 mice that were 12-13 weeks old by 50 µL subcutaneous injections of a myelin oligodendrocyte glycoprotein (MOG 35-55) (MEVGWYRSPFSRVVHLYRNGK; Stanford Pan Facility, Stanford, CA) peptide and complete Freunds adjuvant (CFA) emulsion in four different locations on their back and rear flanks, for a total of 200 µL. … Mice were then injected intraperitoneally with 200 ng of pertussis toxin (List Biologicals, Campbell, CA), on days 0 and 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

8-week old female C57BL/6 mice were immunized for chronic progressive EAE as previously described … In addition, 200ng pertussis (List Biologicals, Campell, CA) was delivered intraperitoneally. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The following drugs were used in this study: pertussis toxin (PTX; List Biological Laboratories Inc., Campbell, CA);…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Five hundred nanograms of Pertussis toxin (List Biologicals, Campbell, CA) in 200 µL of PBS was injected intraperitoneally on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and treatments:

EAE was induced in 8- to 10-week-old mice by subcutaneous immunization with 200 µg MOG 3555 peptide emulsified in complete Freunds adjuvant (CFA, Difco Laboratories) per mouse, followed by administration of 200 ng pertussis toxin (PTX, List biological Laboratories, Inc.) on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Polyclonal CD4+ T cell adoptive transfer:

…In some cases, Foxp3-expressing Treg cells were ablated following adoptive transfer by the i.p. injection of diphtheria toxin (DT; List Biological, Campbell, CA) at a concentration of 0.5 g/mouse on day 1 after adoptive transfer of T cells, and then 0.2 g/mouse every other until day 21. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Mice and Reagents:

… and Diphtheria toxin ( DT ) from Corynebacterium diphtheriae (no. 150) were purchased from List Biological Laboratories Inc. …

TLR stimulation induces a second wave of intimal CD11c+ cell reduction, not mediated by apoptosis, but is dependent on CCR7 and CCL19 expression:

(a) … Positive controls were Cd11c-dtr transgenic mice injected with diphtheria toxin (DT) for 8h.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… The retrograde tract tracers…and cholera toxin B (CTB, 1% solution in a CSF, List Biological Labs, Campbell, CA, USA) were injected into layer V of the cortex…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

CTB (List Biologicals; …was labeled with ATTO488-NHS ester (ATTO-Tec; AD 488-31) or Cy3-NHS ester (using a Cy3 monofunctional dye kit from GE Healthcare; PA33001) according to the manufacturers instructions, and the conjugated products were purified by gel filtration column chromatography on Sephadex-G25 (Nap-5; GE Healthcare; 17-0853-01).

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt