Bacterial Toxin Research Citations

EAE Induction:

Mice (78 weeks) were immunized subcutaneously with 50 or 200 µg of myelin oligodendrocyte glycoprotein (MOG₃₅₅₅; MEVGWYRSPFSRVVHLYRNGK)… Pertussis toxin (List Biological Laboratories, Campbell, CA) in phosphate-buffered saline (PBS; at 200 ng/mouse) was administered intraperitoneally on days of immunization and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

…For active immunization, B6 mice were immunized subcutaneously in both sides of the flank with a total dose of 200 µg MP4 in CFA. Pertussis toxin (List Biological Laboratories, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Quantification of RGC survival after injury:

RGC number was examined after 14 days since ONC. We used the anterograde tracer FITC-conjugated cholera toxin B (CTB-FITC; List Biological, Campbell, CA) to inject into the vitreous chamber of the eye two days before fixation and removal of the eyeball. …

• Product #106 – Cholera Toxin B Subunit (CTB) from Vibrio cholerae FITC, Conjugate

Induction of EAE and anti-VLA-4 mAb treatment:

…EAE was induced by the subcutaneous immunization of 7-week-old C57BL/6 mice with 200 µl emulsions of 200 µg MOG_35-55 peptide (MEVGWYRSPFSRVVHLYRNGK; Protein and Nucleic Acid Facility, Stanford University, Stanford, CA) in Complete Freund’s Adjuvant (4 mg/ml). Additionally, animals received an i.p. injection of 100 µg pertussis toxin (List Biologicals, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunohistochemistry:

Immunohistochemistry was performed on 4 rats injected with the retrograde tracer Cholera toxin B fragment (CtB, List Biologicals, Campbell CA, USA) to identify phrenic motor neurons in the cervical spinal cord. Each rat received an intrapleural injection of CtB for retrograde labeling of spinal phrenic motor neurons using a technique similar to that described previously…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Intranasal vaccination:

… Mice were rested for 4 hours to allow gene expression to peak and subsequently administered with 1µg cholera toxin (CT, List Biologicals) in 10µl PBS. …In summary, each mouse received a total of 9µg of OVA (or GFP) mRNA nanoparticles and 1µg of CT per vaccination.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunohistochemistry and image acquisition:

Primary Antibodies – Goat List 703 1:2000, List Biological Labs

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Experimental Autoimmune Encephalitis:

Mice were immunized with 50 µg of MOG peptide (myelin oligodendrocyte glycoprotein amino acids 3555) emulsified in IFA containing 50 µg Mycobacterium tuberculosis (strain H37RA) by subcutaneous injection. At the time of immunization and three days later, mice received 300 ng of pertussis toxin (List Biological Laboratories) intravenously. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Adoptive Transfer Experiments:

Three groups of mice were infected with ACA (110³) and after 15 days, the animals were sacrificed and cell suspensions were prepared from lymphoid tissues as above. We used two protocols to generate primary T cell cultures: (i) cells were stimulated with concanavalin-A (con-A, 1 g/ml) for two days. …Pertussis toxin (PT; 100 ng; List Biological laboratories, Campbell, CA) was administered i.p. on days 0 and 2 posttransfer, and the animals were monitored for clinical disease. At termination, animals were euthanized, and CNS tissues were collected for histology [16][18].

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

General reagents:

…Anthrax PA83 was purchased from List Biological Laboratories (Campbell, CA, USA). …

MPII proteolysis of proteins in vitro:

Anthrax PA83 (2 g; ~ 1 m) and the following purified human proteins the furin 27107 prodomain (1.8 g, ~ 9 m) [20], the MT1MMP proenzyme (2 g; ~ 2 m), MBP (2 g; ~ 5 m) and BTN3A2 (2 g; ~ 2.5 m) were each coincubated for 1 h at 37 C with increasing concentrations of MPII in 50 mm Hepes, pH 8.0, containing 1 mm CaCl₂, 0.5 mm MgCl₂ and 10 m ZnCl₂. As a control, PA83 and the MT1MMP proenzyme were coincubated for 1 h at 37 C with furin (at the 1 : 100 to 1 : 10 000 enzyme/substrate molar ratio) in 20-L reactions containing 100 mm Hepes, pH 7.5 and 1 mm CaCl₂. …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis