Bacterial Toxin Research Citations

Induction of EAE:

C57BL/6 mice were immunized by subcutaneous injection of MOG_35-55 (200µg) with Freund’s complete adjuvant containing Mycobacterium tuberculosis H37Ra (Difco, Detroit, MI, USA) on the hind flanks. Pertussis toxin (PT; 200 ng; List Biological Laboratories, Campbell, CA, USA) was i.v. on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction and Evaluation:

EAE in mice was induced as described previously [24], [26]. Briefly, 6-8 weeks-old female B6 mice were immunized subcutaneously in the flanks with 100 µg of MOG3555 (MEVGWYRSPFSRVVHLYRNGK) or PLP178191 (NTWTTCQSIAFPSK, UT Southwestern Protein Chemistry Technology Center) emulsified in complete Freunds adjuvant (CFA) supplemented with 4 mg/ml Mycobacterium tuberculosis (MTB, H37Ra, Difco). On days 0 and 2 post-immunization, 250 ng of Pertussis toxin (PTX, List Biological Laboratories) was administered intraperitoneally in 100 µl of phosphate buffered saline (PBS). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
style=”color:rgb(63, 116, 185)”>Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

…To confirm the targets of the projections an additional group of male hamsters received an injection of the retrograde tracer: cholera toxin B (CTB, 0.25% w/v, List Biological, Campbell, CA, USA) in phosphate-buffered saline (PBS: pH 7.4) using the iontophoresis parameters …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Diphtheria toxin treatment:

Diphtheria toxin (DT) was from List Biological Laboratories (Cat. No. 150), reconstituted at 1 mg/ml in PBS, and frozen at 80C. Mice received 10 ng/g DT via the i.p. route in 0.20.3 ml PBS [25].

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Plasmids and RNAi:

Additional siRNAs were tested for LGN and CYK4, with a similar impact on anaphase NuMA/p150 Glued localization. Cells were incu- bated with Pertussis toxin (List Biologicals, Lot#181214A1) at 400 ng/ml for 4 h before analysis.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Oral immunization:

Mice received sodium bicarbonate to neutralize gastric acid before oral immunization (9, 22). Thirty minutes later, the mice were immunized orally concurrently with 1 mg OVA (Sigma-Aldrich) and 10 mg cholera toxin (CT; List Biological Laboratories, Campbell, CA). This oral immunization procedure was conducted on days 0, 7, and 14.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Detection of Serum Antibodies to B. pertussis Antigens:

Nunc Maxisorp 96-well plates were coated overnight with 0.2 g/mL pertussis toxin (PT), 0.5 g/mL filamentous hemagglutinin (FHA), or 2 g/mL pertactin (PRN; List Biologicals, Campbell, CA) as described previously…

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

• Product #187 – Pertactin from B. pertussis (69 kDa Protein)

Product #170 is currently out of stock.  Large quantities may be obtained through Bulk Requests and Customer Orders.

EAE:

…containing 500 µg of the heat-killed H37Ra strain of Mycobacterium tuberculosis (Difco, Detroit, USA). 200 ng of pertussis toxin (List Biological Laboratories, CA, USA) in 100 µl PBS was administered iv immediately after immunization and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Ovalbumin food allergy model:

The OVA food allergy model has been described previously( 16 , 24 ). Briefly, 6-week-old conventional female BALB/c OlaHsd mice (10–15 animals per group) (Harlan Laboratories) were sensitised via the oral route daily during the first 3 d and then at weekly intervals with 20 mg of OVA (Sigma) along with 10 μg/mouse of cholera toxin used as an adjuvant (List Biological Laboratories) for a continuous period of 7 weeks. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Analysis of T-cell and monocyte population activation:

…Monocyte populations in the PBMC samples (200 l per well) were stimulated in separate ninety-six-well plates with 10 g/l of ultra-pure lipopolysaccharide (LPS; List Biological Laboratories, Inc.) per well or an equal volume of sterile endotoxin-free water as a negative control. PBMC were incubated at 37°C in 5 % CO₂ and the supernatants were removed after 24, 48 and 72 h for the analysis of monocyte-derived cytokines as described below.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4