Citations

 Construction of the 3D skin:

… 1 nM cholera toxin (List biological laboratories, Inc., Campbell, CA, USA); …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell Culture

… 100 ng/ml cholera toxin (List Biological Laboratories, Inc.), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materialien und Durchfhrung:

Pertussis toxin List Biological Laboratories, Campbell, US …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis (EAE) model:

C57Bl/6 non-transgenic and shCD5ETg mice were immunized with a single s.c. injection of 150 µg of MOG₃₅₅₅ peptide (BioSynthesis, USA) emulsified in CFA (Difco, supplemented with 400 µg/mL of Mycobacterium tuberculosis) on day 0, followed by one i.p. injection of 200 ng pertussis toxin (LIST Biologicals, USA) on day 0 and another on day 2…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

VGLUT1 contacts on vertical cells in rats that had received sciatic nerve injections:

…The slices from these rats were initially incubated in avidin conjugated to Alexa 488 (Life Technologies; 1:500). They were then cut into 60 m thick sections with a vibrating microtome, and these were scanned to reveal the morphology of the recorded cells. Three of these were classified as vertical cells, and sections that contained most of the dendritic trees of these cells were incubated for 3 days in a cocktail consisting of guinea pig or rabbit antibody against VGLUT1 (Millipore; 1:5,000 and Synaptic Systems, Gttingen, Germany; 1:5,000, respectively) and goat anti-CTb (List Biological Laboratories, Campbell, CA, USA; 1:500). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Retrograde labeling of ascending projection neurons in the brainstem:

After 2 d of survival, mice were transcardially perfused and transverse brainstem sections (20 m thickness) were prepared using a freezing microtome. For fluorescent localization, sections were incubated successively with 10% normal rabbit serum (NRS) in PBS-X containing 0.12% -carrageenan, 1% NRS, and 0.02% sodium azide (PBS-XCR) for 30 min, 1:10,000 goat anti-CTB antibody in PBS-XCR (703; List Biological Laboratories…

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… CT ( Choleratoxin ) List Biological Laboratories, Inc. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Intracellular detection of LFn fusion proteins and PARP cleavage:

… Detection of toxin proteins was achieved with rabbit anti-LF and mouse anti-CdtB, both made in our laboratory, and goat anti-Pseudomonas exotoxin A (List Biological Laboratories, Campbell, CA, USA). …

• Product #760 – Anti-Exotoxin A (Goat) from Pseudomonas aeruginosa

Induction and Clinical Assessment of EAE:

…Mice were then immediately injected intravenously with 350 ng of pertussis vaccine (List Biological Laboratories, Campbell, USA) and again 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Intracortical tetanus and botulinum toxin injections:

For intracortical injections, a craniotomy is made over the cingulate cortex of anesthetized mice by thinning the skull with a dental drill and using a fine needle to remove a small (100 m diameter) piece of skull. Using a fine glass capillary mounted to a stereotaxic apparatus, tetanus toxin³² (25ng; 190A, List Biological Labs) or botulinum toxin (0.03ng; 128A, List Biological Labs) was diluted in 7 l of artificial cerebral spinal fluid (ACSF) and injected 500 m into the cingulate cortex.

• Product #190 – Tetanus Toxin from Clostridium tetani
• Product #128 – Botulinum Neurotoxin Type A Complex from Clostridium botulinum