Bacterial Toxin Research Citations

Cholera Toxin Labeling:

Three days later, mice were deeply anesthetized with 1 ml Avertin and transcardially perfused with 4% formalin. Brains were removed, postfixed…incubated in goat anti-CTb (List Biologicals, 1:8,000) overnight at 4C, and visualized with rabbit anti-goat Vectastain horseradish peroxidase

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE induction (mice) – On day 0 and day 2 post-immunization, 200 ng pertussis toxin (List Biologicals) was administered intraperitoneally.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 21. Pertussis toxin (PTx, List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 500 ng of pertussis toxin (islet activating protein, i.p.; List Biological Laboratories, Inc., Campbell, CA) was utilized for animal administration at the time of immunization and again 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Study animals:

… Individual foals were randomly assigned to the following groups: 1) EBRE 1 group (n=9) which received 21010 CFUs of R. equi inactivated by 4 kGy of e-beam radiation  in 100 ml of saline adjuvanted with 100 g of
cholera toxin B subunit (CTB, List Biological Laboratories, Campbell, CA, USA); 2) EBRE 2 group (n=10) which received 11011 CFUs of R. equi inactivated by 5 kGy of e-beam radiation in 100 ml of saline adjuvanted with 100 g of CTB; 3) Saline (negative) control group (n=9) which received 100 ml of saline adjuvanted with 100 g of CTB; and, 4) LVRE (positive) control group (n=6) which received 11010 CFUs of live R. equi in 100 ml of saline. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Engraftment of mice with human PBMCs:

Female C.B-17 SCID mice (Taconic) were maintained in microisolator cages (Innovive) and used at 10 weeks of age. The engraftment of human PBMCs into SCID mice was performed essentially as previously described ([25, 26]). … On day 10, 10 mice in each group were injected intraperitoneally with 15 g of tetanus toxoid (List Biological Laboratories) in PBS. On the days indicated, blood was collected, and serum was assayed for human IgM, IgG2, and anti-tetanus IgG (IBL). …

• Product #191 – Tetanus Toxoid from Clostridium tetani

Cytotoxicity assay:

PA and LF were purchased from List Biological Laboratories, Inc., …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Immunization and immunogenicity studies:

For intranasal immunizations, 4 µg of the indicated recombinant protein with 1 µg cholera toxin (CT; List Biological Laboratories Inc.) per dose was administered in a 20 µl volume in sterile phosphate-buffered saline (PBS). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Tissue Processing, Analysis, and Immunohistochemistry:

Nucleus ambiguus.

…The sections were then incubated in goat anti-CTB antiserum (1:25,000; List Biologicals) in TPBS-Triton containing 10% NHS for 3 days at room temperature. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Animals and Diets:

Male APOE3L.CETP mice were 1014 weeks old…

… Groups were additionally treated with one of the following treatments until week 16: LPS from Salmonella minnesota R595 (lot. no. 30446A1; List-Biological Laboratories, Campbell CA, USA) by minipump at 5g per day; recombinant murine IL-1…

• Product #304 – LPS from Salmonella minnesota R595 (Re)