Citations

… A 50 L of anthrax toxin PA63 (List Biological Labs, Campbell, CA, USA) dissolved in 50 mM carbonatebicarbonate buffer (pH 9.6) was used to coat each well of a 96-well plate…

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

Measurement of anti-Diphtheria IgG by Luminex

Immune response to Diphtheria toxoid (DT) carrier protein was assessed at Days 0, 7 and 30 using anti- DT IgG capture microsphere assay. Fluorescent microspheres (Luminex Corp, Austin, TX) were covalently conjugated to DT (List Biologicals, Campbell, CA) and used to capture and quantify DT specific IgG in sera based on previous method with minor modifications.

• Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae

Cell depletion strategies:

… For depletion of CCR2⁺ cells, CCR2-DTR mice and control CCR2-DTR negative littermates received 250 ng of diphtheria toxin i.p. one day prior to infection and every other day thereafter in order to maintain depletion. Diphtheria Toxin was purchased from List Biological Laboratories (Campbell, CA), and reconstituted at 1 mg/ml in PBS. Aliquots were stored in 80C. The specificity and efficiency of depletion in the lung was confirmed by flowcytometric analysis.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Purified diphtheria toxin was obtained from List Biological Laboratories Inc. (Campbell, CA).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

EAE induction:

EAE was induced by active immunization with MOG_35-55 peptide… Mice were also injected intraperitoneally with 500 ng pertussis toxin (List Laboratories) in phosphate buffered saline…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunohistochemistry:

… However, the retrograde staining of preganglionic cardiac motoneurons was minimal using FG, so pericardial injections of Cholera Toxin B Subunit (CTB) Conjugate (List Laboratories, Inc.) were made in the other 16 rats (13 juvenile and 3 adult), but only after the juvenile rats first had been trained to dive underwater (vide supra). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Experimental Autoimmune Encephalomyelitis (EAE):

Induction of EAE in C57BL/6 mice (N =18) was used as a model for multiple sclerosis (MS). EAE was induced by subcutaneous injections of 100 µg of myelin oligodendrocyte glycoprotein (MOG₃₅₅₅; MEVGWYRSPFSRVVHLYRNGK, AnaSpec Inc., Fremont, CA) in complete Freunds adjuvant (incomplete Freunds adjuvant containing 4 mg/ml of heat-inactivated Mycobacterium tuberculosis H37Ra; BD Biosciences, Mississauga, ON, Canada). An intravenous injection of 200 ng of pertussis toxin (PTX; List Biological Laboratories Inc., Campbell, CA) was also administered on days 0 and 2 of the immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

… Mice were also injected intraperitoneally with 400 ng of pertussis toxin (List Biological Laboratories, Inc., Campbell, CA, USA) on days 0 and 2 after MOG sensitization …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice, peptides and EAE induction:

…EAE was induced as described [17] by subcutaneous immunization with MOG₃₅₅₅ (100 g/mouse) in Complete Freund’s Adjuvant (CFA, Difco), containing 4 mg/ml of heat-killed Mycobacterium tuberculosis (Difco). All mice were injected i.v. with 200 ng/mouse of Bordetella Pertussis toxin (PTX, List Biological Lab) on day 0 and 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and treatment:

…Chronic EAE was induced in the female C57Bl/6 mice by subcutaneous immunization with MOG₃₅₅₅ (Anaspec, San Diego, CA), which was emulsified in Complete Freund’s adjuvant with 8 mg/ml of Mycobacterium tuberculosis H35RA (Difco, Detroit, MI) in 11 ratio, and 100 µl (100 ng) of emulsified MOG₃₅₅₅ was injected sub-cutaneously at each side of the base of the tail respectively. Each mouse also received 100 µl (200 ng) of pertussis toxin (List Biological Laboratories, Campbell, CA) by intraperitoneal (i.p.) injection, and the injection of toxin was repeated 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)