Bacterial Toxin Research Citations

EAE induction and in vivo treatment with JTE-013:

EAE was induced in SJL/J mice via active immunization with proteolipid protein…In addition, mice were injected with 200 ng pertussis toxin (List Biologicals Laboratories Inc.) at the time of immunization and 2 days after.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials:

… Activated protective antigen [63 kDa (1 M=63g/ml); PA63] and recombinant lethal factor [90 kDa (1 M=90 g/ml); LF] were obtained from List Biological Laboratories (Campbell, CA). …

PA63 and LF were used in the following:

• Optimization of LTx complex detection assay
• Limit of detection and quantification
• Precision and Accuracy for PA83 and PA63 Detection
• Detection of PA63 and LTx in the presence of AIG

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

… bilateral injections of two different tracers into the OB and prepiriform cortex, namely, biotinylated dextran amine (BDA; 10,000 molecular weight; Molecular Probes, Eugene, OR) on the right and cholera toxin B subunit (CTB; choleragenoid; List Biological Laboratories, Campbell …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

…either singly or as a mixture with one retrograde tracer, either Cholera Toxin-B subunit (CTB; 0.5% diluted in distilled water; List Biological)…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Reagents:

… Ultrapure LPS from Salmonella minnesota R595 (Re) and Ultrapure LPS from Escherichia coli 055:B5 were obtained from List Laboratories. …

Human Macrophage Assay with LPS and Nickel:

… Five days later, the differentiated macrophages were incubated with a dose range of Hu 15C1 together with ultrapure LPS from S. minnesota (1, 10, or 100 ng/ml; List Biological Laboratories)…

U937 Cell Assays:

… The cells were subsequently incubated with 1 ng/ml ultrapure LPS from S. minnesota for 20 h, and IL-6 in the supernatants was quantified using the Quantikine ELISA kit (R&D Systems).

Confocal Microscopy and FRET Analysis:

THP-1 cells either untreated or treated with ultrapure LPS from E. coli (100 ng/ml) in the presence or absence of different concentrations (0.1, 1, or 10 g/ml) of Hu 15C1…

THP-1 Blue Prolonged Blockade Assay:

… The cells were resuspended in fresh medium and incubated for 0, 1, or 24 h prior to stimulation with 5 ng/ml ultrapure LPS from S. minnesota for 24 h. NF-B reporter gene activation, …

LPS Lung Instillation:

C57BL/6 mice (female, 8 weeks old) from Charles River Laboratories were injected with the indicated antibodies intravenously. One hour later, mice were anesthetized and instilled intranasally (i.n.) with 10 g of ultrapure LPS from S. minnesota. 24 h post-LPS challenge, mice were sacrificed, and bronchoalveolar lavage (BAL) was performed…

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5
• Product #434 – ULTRA PURE LPS from Salmonella minnesotaR595 (Re)

Induction and evaluation of experimental autoimmune encephalomyelitis (EAE):

EAE was induced with an adaptation of the method of Bettelli et al. [21]. Eight to 16 weeks old Mst1^/ and Mst1^+/+ littermates were immunized subcutaneously with a total of 300 µg MOGp3555 emulsified in CFA containing 250 µg heat-inactivated Mycobacterium tuberculosis H37Ra (BD Biosciences). Immediately following the injection of the emulsified Ag (day 0), each mouse received one intravenous injection of 500 ng of Pertussis toxin (List Biological Laboratories, Campbell, CA). Disease severity was scored on a scale of 0 to 8 as previously described [22]. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunization with YopE and ovalbumin peptides:

Unless otherwise indicated, mice were lightly anesthetized with isoflurane and immunized intranasally on days 0, 7 and 21 with 15 µl saline solution containing 1 µg cholera toxin (CT; List Biological Laboratory, Campbell, CA) as adjuvant …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Diphtheria toxin administration:

Diphtheria toxin (List Biologicals Laboratories, Campbell, CA) was suspended in phosphate-buffered saline. Stock solutions were thawed once and mice were injected i.p. with 50 or 10gkg¹ of diphtheria toxin similar as previously described.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Toxin inactivation and formulation:

TcdA and TcdB (purified from C. difficile strain VPI10463, List Biologicals, Campbell, CA) and selected recombinant fragments (B-ED and B-TC) were reconstituted in 20 mM HEPES-buffered saline (pH 7.4) at 0.30.4 mg/mL. …

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

Cholera toxin subunit b immunostaining:

Coronal sections (50 m) of gelatin-embedded cerebella and brainstems were cut on a Peltier freezing microtome and collected in 0.01 m PB. CTb immunostaining was carried out using a standard immunostaining protocol. Free-floating sections were processed for anti-CTb immunostaining using a primary polyclonal goat anti-CTb antibody (List Biological Laboratories, Campbell, CA, USA; 1:15,000) and a …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)