Bacterial Toxin Research Citations

Histology and Immunohistochemistry

… For mapping of Cholera Toxin Subunit B-injected brains, at the time of ICH induction, 0.5 μL of retrograde neuronal tracer Cholera Toxin Subunit B (CTb, List Biological Laboratories, Campbell, CA, USA, diluted to 20 μg/μL in 10% DMSO) was infused with collagenase, blood, or alone. Seven days after treatment, animals were killed, and brains were fixed, frozen, and coronally sectioned at 50 μm. A series of six sections spaced 200 μm apart were blocked in 5% normal donkey serum and 0.3% Triton, incubated with a CTb primary antibody (Goat anti-CTb, 1:500, List Biological Laboratories) overnight,

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Experimental Autoimmune Encephalomyelitis:

EAE immunization was performed as previously described … Mice received 200 ng of pertussis toxin (List Biologicals) intraperitoneally on day 0 and 1 after immunization. Two investigators, blinded to the genotype of mice, independently analyzed the mice daily on a 5-point scale: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalitis induction and treatment protocols:

… Mice also received 100 µl of 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA, USA) by intraperitoneal injection concomitantly and again 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Study animals:

Thirty-four healthy Quarter Horse foals and their respective dams were used for this study. …Individual foals were randomly assigned to the following groups: 1) EBRE 1 group (n=9) which received 210¹⁰ CFUs of R. equi inactivated by 4 kGy of eBeam radiation in 100 ml of saline adjuvanted with 100 µg of cholera toxin B subunit (CTB, List Biological Laboratories, Campbell, CA, USA); …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Cell culture studies:

…Mouse peritoneal macrophages where isolated from C57BL/6J, C3H/HeJ and CD44 ^-/- four days after an IP injection of thioglycolate as previously described [36]. …Cells were grown in DMEM…overnight, rinsed with PBS and then adherent cells treated in 1% FBS alone as a control or stimulated with LPS (List Biological Inc., Campbell, CA, #201). Cells were treated for 4 hours except for time-course experiments. …

• Product #201 – LPS from Escherichia coli O111:B4

Induction of active EAE in mice by immunization with MOG and adjuvant:

EAE was induced by procedures previously described in female wild-type C57BL/6J mice …All mice were administered 400 ng pertussis toxin (List Biological) i.p. at 0 and 48 h after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… PT, List Biologicals Laboratories, Campbell, CA, 181, Store at 4° C. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Induction of EAE:

C57BL/6 mice were immunized by subcutaneous injection of MOG_35-55 (200µg) with Freund’s complete adjuvant containing Mycobacterium tuberculosis H37Ra (Difco, Detroit, MI, USA) on the hind flanks. Pertussis toxin (PT; 200 ng; List Biological Laboratories, Campbell, CA, USA) was i.v. on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction and Evaluation:

EAE in mice was induced as described previously [24], [26]. Briefly, 6-8 weeks-old female B6 mice were immunized subcutaneously in the flanks with 100 µg of MOG3555 (MEVGWYRSPFSRVVHLYRNGK) or PLP178191 (NTWTTCQSIAFPSK, UT Southwestern Protein Chemistry Technology Center) emulsified in complete Freunds adjuvant (CFA) supplemented with 4 mg/ml Mycobacterium tuberculosis (MTB, H37Ra, Difco). On days 0 and 2 post-immunization, 250 ng of Pertussis toxin (PTX, List Biological Laboratories) was administered intraperitoneally in 100 µl of phosphate buffered saline (PBS). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
style=”color:rgb(63, 116, 185)”>Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

…To confirm the targets of the projections an additional group of male hamsters received an injection of the retrograde tracer: cholera toxin B (CTB, 0.25% w/v, List Biological, Campbell, CA, USA) in phosphate-buffered saline (PBS: pH 7.4) using the iontophoresis parameters …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt