Citations

Tract tracing and immunofluorescence:

Retrograde tract tracing was performed using a 1% solution of cholera toxin B subunit (CTB; List Biological Labs), injected using standard stereotaxic coordinates…Large areal injections of CTB were performed by pressure injection using a 33 ga stainless steel needle,…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE Induction:

For the induction of EAE, C57BL/6 mice received a single immunization of MOG35-55 in Freunds complete adjuvant containing killed Mycobacterium tuberculosis H37Ra subcutaneously on day 0, followed by intravenous injection with 200 ng of pertussis toxin (List Biological Laboratories) on day 0 and 2 [39]. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials and methods – Reagents:

… Re-extracted LPS from Escherichia coli was purchased from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

In Vitro Migration and Actin Polymerization

Migration assays were performed using 24-well transwell plates with 5-μm pore size filter inserts (Corning). Prior to migration assays, L1.2 or THP-1 cells were pretreated with either 100 μm BX-471 (kind gift of Richard Horuk, University of California, Davis), 0.2 μg/ml pertussis toxin (List Biological Laboratories), or DMSO as a control for 1 h at 37 °C. …

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

… Animals in the IN group immunized with recombinant MOMP (100 µg) and either … or CT (List Biological Laboratories, Campbell, CA, USA) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Pharmacological injections: 

Pharmacological compounds were prepared in sterile 0.9% saline and stored at 20 C prior to use. Diptheria toxin (DT) (50 g/kg; List Biologicals, #150), was injected intramuscularly (in mice) and all other compounds were administered intraperitoneally.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Animals and vaccinations:

…Cholera toxin (List Labs; Campbell, USA) was administered at 2 µg/mouse in 50 µL mixed with the vaccine.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

INDUCTION OF EAE

EAE was induced as previously described (27). Briefly, eight-week-old female SJL/J mice, each weighing 20g (20±1g) were anesthetized with ketamine/xylazine and were injected subcutaneously at two sites on the back with 0.1 mL solution containing 0.5 mg/mL proteolipid protein peptide 139–151 (PLP; GenScript, Piscataway, NJ) emulsified in complete Freund’s adjuvant (CFA; Difco, Detroit, MI) containing 0.5 mg/mL mycobacterium tuberculosis (Difco). Control mice were injected with equal volumes of PBS and CFA. All mice were injected with 200 ng pertussis toxin (List Biological, Campbell, CA) in 0.1 mL PBS intraperitoneally on day 0 (day of immunization) and again on day 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials and Methods:

… Recombinant protective antigen (rPA) of recent production from List Biological Laboratories Inc. was used as a control for these studies.

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

… On a separate plate, two – fold dilutions of Fab in serum – free media plus 400pM of native TcdA ( List Biological Laboratories, Inc., cat# 152B) was pre – incubated for 1 hour and then the mixture was added to the cells by media exchan ge. After 24 hours (when cell round ing in controls became evident), the media was changed to complete media (EMEM with serum). …

• Product #152 – Toxin A from Clostridium difficile