Citations

In Vivo Model of Recurrent LPS Exposure:

Escherichia coli LPS type 0111:B4 (List Biologicals, Campbell, CA) was used for animal exposures. LPS was delivered by noninvasive oral aspiration, as described previously (13). Briefly, mice were lightly anesthetized and suspended by upper incisors on a 45-angle incline board. The tongue was extended with forceps and 100 l of LPS or PBS was placed into the oral cavity. Mice were monitored until fluid aspiration followed by several additional chest retractions was observed; 10 to 12 mice of each genotype were exposed for each end point. Mice were administered either a single 8-g dose of LPS to assess threshold sensitivity due to SP-C deficiency or were serially administered 8 g LPS interspaced by 2 days of recovery before the second and third doses. Mice were observed for 3, 5, or 30 days after the final LPS exposure before assessing recovery from injury. In a separate experiment, an SP-Cenriched surfactant extract was transiently replaced before LPS challenge. Mice were given a single 25-g dose of Survanta (Abbvie Inc., North Chicago, IL) as an exogenous source of SP-C before LPS exposure, and the cell counts and myeloperoxidase activity levels determined in the bronchoalveolar lavage fluid (BALF) 24 hours later as an indicator of neutrophil activity and cellular inflammation.

• Product #201 – LPS from Escherichia coli O111:B4

Key reagents and their sources were as follows: Escherichia coli LPS serotype O1101:B4 (List Biological Laboratories),…

Isolation, culture, and experimental testing of BMDC:

For experimental tests, plated BMDC were centrifuged at 300 3 g for 510 min, and the differentiation medium was removed and replaced with low-serum DMEM (0.1% bovine calf serum plus penicillin, streptomycin, and L-glutamine). The cells were equilibrated for 15 min at 37C in 10% CO2 prior to addition of test reagents. BMDC were routinely primed with 1 mg/ml LPS (unless indicated otherwise) for 4 h to activate TLR4 signaling prior to treatment with indicated concentrations of Dox, STS, or other chemotherapeutic drugs for additional periods ranging from 2 to 18 h (with the LPS being present throughout).

• Product #201 – LPS from Escherichia coli O111:B4

EAE:

…Mice received 20 ng of pertussis toxin (PTX; List Biological Laboratories) intravenously at the time of immunization and 48 hr later. For passive-transfer EAE induction, C57Bl/6J WT mice were injected with 20 ng PTX 4 days and 1 day before cell transfer.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

… Purified pertussis toxin (PTX) was purchased from List Biological Laboratories (Campbell, CA). …

Induction and Scoring of EAU:

Mice were immunized by injection of 0.2 mL emulsion containing 40 µg bovine whole IRBP in CFA, administered by subcutaneous injections into the tail base and the two thighs. PTX, 0.2 µg, was injected intraperitoneally, concurrently with the immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.2. EAE immunization:

C57Bl/6 mice were immunized subcutaneously with 200 µg of MOG₃₅₅₅ … Pertussis toxin (List Biological Laboratories, Campbell, CA) (250 ng) was injected i.p. on the day of immunization and 2 days later. Mice were monitored daily for clinical signs of disease and were scored as follows: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and treatment of EAE:

Active induction of EAE involved s.c. injection of 100 µg MOG₃₅₅₅ MEVGWYRSP-FSRVVHLYRNGK… Each mouse also received 300 ng of Bordetella PT(List Biological Laboratories) i.p. on day 0 and 2 postimmunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and assessment of EAE:

Additionally, the mice received 100 ng of pertussis toxin (List Biological Laboratories, Inc.) i.p. on days 0 and 2. Mice were monitored daily and scored for disease severity using the standard scale…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE, treatment with THI, immune cell proliferation and cytokine analysis

EAE was induced in 8-9 weeks-old female WT or S1P₁(S5A) mice (n = 10 per group) immunized with Complete Freunds Adjuvant (CFA) and Myelin Oligoglycoprotein (MOG) peptide₃₅₅₅ and Bordetella pertussis toxin (List Biological Laboratories) 400 ng per mouse on days 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Dorsal column primary sensory axon tracing:

Dorsal column sensory projections from the sciatic nerve to the nucleus gracilis in the medualla were traced with choleratoxin B (CTB)… Two µl of 1% cholera toxin B (CTB; List Biologicals, Campbell CA) solution was then slowly injected over 1 minute and the needle left in place for an additional 2 minutes. The needle was then withdrawn from the nerve and the ligature removed. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Experimental Materials:

Stx2 toxin was purchased from List Biological Laboratories, Inc. (Lot #1621A1, Campbell, CA). Endotoxin levels were tested by List Biological Laboratories and found to be acceptable. …

Determination of Mean Lethal Dose:

Groups of at least 10 randomly selected mice were treated by intraperitoneal (ip) injection with 500 L per dose of serial dilutions of Stx2 (in a range that spans high lethality to no deaths). …

Toxicokinetics of Stx2:

The biologic half-lives of Stx2 were determined in the presence or absence of mAbs against Stx2. Mice were treated iv with 100 ng per mouse (100 L of 1,000 ng/mL stock) of Stx2. …

Treatment of Mice Post-intoxication or Pre-intoxication with Stx2 mAbs:

To simulate post-intoxication treatment, mice were treated by iv with 100 L of 180 ng/mL of Stx2. …

List Labs has discontinued Product #162 (Lyophilized) and has replaced Product #162 with Product #162L (Liquid):

• Product #162L (Liquid) – Shiga Toxin 2 from Escherichia coli