Bacterial Toxin Research Citations

SNAPtide Assay[44]

Recombinant 425aa BoNT/A LC was used throughout all assays and was prepared as previously described.[45] All SNAPtide assays were run in 40 mM HEPES + 0.1% Triton X-100 at pH 7.4. BoNT/A LC concentrations were 18.5 nM while SNAPtide (prod. no. 521, List Labs) concentrations were 5 M. Fluorescence was recorded continuously for 1.5 h to calculate an accurate k_obs using equation 1. In the preincubation experiments, inhibitors at the specified concentrations were incubated with 925 nM enzyme and diluted 50-fold into substrate and initial rates of SNAPtide cleavage were measured. …

• Product #521 – SNAPtide Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin

EAE:

…0.2 ml inoculum containing 200 µg MOG peptide 3555 (MEVGWYRSPFSRVVHLYRNGK) in IFA (DifcoLabs, Detroit, MI) with 800 µg Mycobacterium tuberculosus hominis H37Ra (MT) on day 0 and 7 following [23], with pertussis toxin (PTx) (List Biologicals) 200 ng ip on …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and IVIg treatment:

Mice (8-10 wks of age) were immunized by subcutaneous injection of mice at two sites in the chest (50 µl/side) with an emulsion containing myelin oligodendrocyte glycoprotein amino acids 3555 (MOG₃₅₅₅) (2 mg/ml) (Stanford University Pan Facility, Stanford, CA) with Complete Freunds Adjuvant (CFA) containing heat killed Mycobacterium tuberculosis H37Ra (4 mg/ml; Difco, Detroit, MI) [29]. Mice were also injected i.p. with 75 ng of pertussis toxin (List Biologicals, Campbell, CA) on the day of and 2 days after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…emulsified 1:1 in Complete Freunds Adjuvant (CFA) to each hind flank (50 µL each), in addition to intravenous Pertussis toxin injection (300 ng in endotoxin-free PBS) (List Biological Laboratories, Campbell, CA …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and clinical evaluation of disease:

For active EAE induction, mice were immunized s.c. with 100-150 mg PLP139151 or myelin oligodendrocyte glycoproteins (MOG) 3555 emulsied in CFA; 0.15 mg pertussis toxin (List Biological, Campbell, CA) was injected i.p. in 200 ml saline on days 0 and 2. Mice were observed daily for the clinical appearance of EAE.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE to TgMHu₂ME199K mice:

…On the day of inoculation and 48 h later, 100 ng of pertussis toxin (List Biological Laboratories, Inc., Cambell, CA, USA) in 0.1 ml saline was also administered by intraperitoneal injection.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Toxin challenge:

We performed an in vivo challenge experiment with CT, as described previously (Tokuhara et al. 2010). The vaccinated mice (ten per group) were orally challenged with 20 µg of CT (List Biological Laboratories, Campbell, CA, USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction:

EAE was elicited by subcutaneous (s.c.) tail-base injection of 50 µg of MOG3555 peptide (35-MEVGWYRSPFSRVVHLYRNGK-55) (U-M Protemocis and Peptide synthesis core facility) in complete Freund adjuvant (CFA) containing 500 µg of heat-inactivated Mycobacterium tuberculosis (serotype H37RA) (DIFCO Labs). A dose of 200 ng of B. pertussis toxin (LIST Biological Labs, Inc.) was injected intra peritoneally (i.p.) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Peanut allergy model (in mice):

5 µg CT (List Biologicals) was administered i.g. (Delvo), i.p., p.r., or s.c. weekly for 4 wk, with challenge by crude PN extract (CPE; Greer) 1 wk later.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… 1). Mice were sensitized intragastrically (ig) with 20 mg whey (DMV International, Veghel, The Netherlands) in 0.5 mL PBS, containing 10 µg cholera toxin (CT, List Biological Laboratories, Campbell, CA, USA) as an adjuvant. Sham-sensitized mice received CT alone. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae