Bacterial Toxin Research Citations

EAE model induction and treatment:

EAE was induced as follows: mice (age 6 to 8 wk) were immunized subcutaneously (s.c.) with a mixture of myelin oligodendrocyte glycoprotein…Pertussis toxin (200 ng/mouse, List Biological Laboratories, 181) in PBS was administered i.v. on day 0 and day 2.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE:

Active optimal or sub-optimal EAE was induced with sub-cutaneous (s.c.) injection of 200 or 160 µg MOG3555 peptide (Peptides International) respectively in 150 µl of Complete Freunds Adjuvant (Sigma-Aldrich). On day 0 and 2 postimmunization, intraperitoneal (i.p.) injection of 400 or 300 ng of Bordetella pertussis toxin (PT; List Biological Laboratories) was respectively performed for optimal or sub-optimal EAE induction. EAE severity was scored daily using the standard scale. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis (EAE) mouse model:

… The mice in the EAE group were immunized with 50 µg MOG_35-55 peptide emulsified (1:1) in incomplete Freunds adjuvant (IFA) and desiccated Mycobacterium tuberculosis. Pertussis toxin (300 ng; PTX, List Laboratories, Campbell, CA) was injected intravenously on the day of MOG_35-55 immunization and two days later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and evaluation of EAE:

…On the day of immunization and 48 h later, the mice were also injected i.v. with pertussis toxin (200 ng/ mouse; List Biological Laboratories, Campbell, CA) in PBS. Mice were examined daily for EAE disease symptoms, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Each animal received bilateral injections of cholera toxin B subunit (CTB, choleragenoid, List Biological Laboratories, Campbell, USA) in either the entopallium or field L2. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

In vivo extracellular injections:

Injections of 310 nL of a 1% solution of cholera toxin subunit b (CTb) (List Labs, Campbell, CA) were performed in 10 pigeons. The targets of these injections were the eye chamber (two cases), the GLv (five cases), and the TeO (three cases). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… The tip of a glass canula sealed to a Hamilton syringe was inserted through the incision and 3.5 µl of a 1% cholera toxin B subunit solution (CTB; List Biological Laboratories) were slowly injected over a 3 min time period. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Induction of EAM and DCMi:

On day 0, mice also received 500 ng pertussis toxin i.p. (List Biologicals).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

8-wk-old female mice were immunized by s.c. injection at the base of the tail…The mice also received i.v. injections of 200 ng pertussis toxin (List Biological Laboratories) on days 0 and 2 and were administered i.p. 100 µg anti4-1BB (clone 3H3) or control antibody (rat IgG) on days 0, 2, 4, and 8.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

EAE was induced as described [19]. Female C57BL/6 mice were subcutaneously injected with 100 µg of MOG3555 (myelin oligodendrocyte glycoprotein; AnaSpec) in IFA (incomplete Freund’s adjuvant; Difco) containing 250 µg of Mycobacterium tuberculosis (Difco). On the same day and again 48 hours later, mice received an intraperitoneal injection of 200 ng of pertussis toxin (List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)