Bacterial Toxin Research Citations

CT ELISA and -Galactosidase Assays:

CT production was measured using V. cholerae culture supernatants by an GM₁-enzyme-linked immunosorbent assay (GM₁-ELISA) as described previously (4). Purified CT subunit B (List Biological Laboratories, Inc., Campbell, CA) was used to provide a standard curve, and phosphate-buffered saline (PBS) was used as a negative control.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

In vitro assay for the effects of DP on macrophages and lymphocytes:

…CCR2 expression on the surface of macrophages after DSP or DP treatment was analyzed by FACS as described previously [19]. Briefly, thioglycolate-recruited peritoneal macrophages were pretreated with 25 M (as dexamethasone) of DSP or DP for 3 hours, and then, were exposed to LPS (O55:B5, 100 ng/mL, List Biological Laboratories, Inc. Campbell, CA) for 18 hours. …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Cholera Toxin Labeling:

Three days later, mice were deeply anesthetized with 1 ml Avertin and transcardially perfused with 4% formalin. Brains were removed, postfixed…incubated in goat anti-CTb (List Biologicals, 1:8,000) overnight at 4C, and visualized with rabbit anti-goat Vectastain horseradish peroxidase

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE induction (mice) – On day 0 and day 2 post-immunization, 200 ng pertussis toxin (List Biologicals) was administered intraperitoneally.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 21. Pertussis toxin (PTx, List Biological Laboratories). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 500 ng of pertussis toxin (islet activating protein, i.p.; List Biological Laboratories, Inc., Campbell, CA) was utilized for animal administration at the time of immunization and again 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Study animals:

… Individual foals were randomly assigned to the following groups: 1) EBRE 1 group (n=9) which received 21010 CFUs of R. equi inactivated by 4 kGy of e-beam radiation  in 100 ml of saline adjuvanted with 100 g of
cholera toxin B subunit (CTB, List Biological Laboratories, Campbell, CA, USA); 2) EBRE 2 group (n=10) which received 11011 CFUs of R. equi inactivated by 5 kGy of e-beam radiation in 100 ml of saline adjuvanted with 100 g of CTB; 3) Saline (negative) control group (n=9) which received 100 ml of saline adjuvanted with 100 g of CTB; and, 4) LVRE (positive) control group (n=6) which received 11010 CFUs of live R. equi in 100 ml of saline. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Engraftment of mice with human PBMCs:

Female C.B-17 SCID mice (Taconic) were maintained in microisolator cages (Innovive) and used at 10 weeks of age. The engraftment of human PBMCs into SCID mice was performed essentially as previously described ([25, 26]). … On day 10, 10 mice in each group were injected intraperitoneally with 15 g of tetanus toxoid (List Biological Laboratories) in PBS. On the days indicated, blood was collected, and serum was assayed for human IgM, IgG2, and anti-tetanus IgG (IBL). …

• Product #191 – Tetanus Toxoid from Clostridium tetani

Cytotoxicity assay:

PA and LF were purchased from List Biological Laboratories, Inc., …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Immunization and immunogenicity studies:

For intranasal immunizations, 4 µg of the indicated recombinant protein with 1 µg cholera toxin (CT; List Biological Laboratories Inc.) per dose was administered in a 20 µl volume in sterile phosphate-buffered saline (PBS). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae