Bacterial Toxin Research Citations

Active and Transfer EAE Induction:

Mice received 20 ng of pertussis toxin (PTX; List Biological Laboratories) intravenously at the time of immunization and 48 hr later. For passive-transfer EAE induction, C57Bl/6J WT mice were injected with 20 ng PTX 4 days and 1 day before cell transfer. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice and Immunization:

… Mice were immunized with 20 230g of each purified Gcf proteins with 2 µg of CT (List Biological Lab. Inc. Campbell, CA) via the sublingual (s.l.) route on day 0 and day 14. As control, mice were immunized with CT via sublingually, 110⁵ PFU of FI-RSV via foot-pad as described by Delgado et al. [24], or 110⁵ PFU of live RSV through intranasal (i.n.) route. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Induction and clinical evaluation of EAE:

Mice 5-7-week-old were immunized s.c. with an emulsion of Complete Freund’s Adjuvant… In addition, 200 ng of Pertussis toxin (List biological laboratories; Campbell, CA, USA) in 200 µL saline solution was injected i.p. on days 0 and 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Animal Experiments:

…Mice were intravenously injected with 0.2 µg of Bordetella pertussis toxin (List Biological Laboratories, Campbell, CA, USA), in a 100 µl final volume, on days 0 and 2 following sensitization. Mice developed a relapsing-remitting clinical course (RR-EAE). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Preparation of protein conjugates of AnAFB₁

The immunogen AnAFB₁, prepared converting AFB₁ (Sigma-Aldrich, St. Louis, MO, USA) to AFB₁-1(O-carboxymethyl) oxime using the method of Chu et al. [17], as previously described [15], was conjugated to KLH (Sigma-Aldrich) or CRM₁₉₇ (List Biological Laboratories Inc., Campbell, CA, USA) to be used as immunogen. …

• Product #149A – Diphtheria Toxin CRM₁₉₇, Mutant

Immunization and induction of EAE:

The mice were also injected intraperitoneally with 200 µg pertussis toxin (List Biological Laboratories) on days 0, 1 and 2. All mice were monitored daily for EAE symptoms and scored…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

On days 0 and 2 challenged mice received 250 ng of Bordetella pertussis toxin IP (List Biological, Campbell, CA).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE and immunizations:

For active EAE induction, mice were immunized subcutaneously with 100µg MOG(3555)…Pertussis toxin (List Biological Laboratories) was injected intraperitoneally (300ng) on days 0 and 2. Mice were observed for signs of EAE…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents and Instruments:

…LPS from E. coli O111:B4 was obtained from LIST Biological Laboratories. …

LPS stimulation, Cytokine Production, and NF-B Activation:

Concentrations of secreted IL-8 in the supernatants after LPS stimulation were measured by ELISA. Briefly, 310⁵ cells of each cell line were seeded in 24 well plates in complete media containing 100 g/ml Zeocin. After 1620 hours incubation, cells were stimulated with 0, 0.1, 0.5, 2 and 20 ng/L LPS for 24 hours. Concentrations of human IL-8 were measured by Human CXCL8/IL-8 DuoSet ELISA kit. Each experiment was repeated three times.

Intracellular concentrations of human IL-8, phosphorylated and total NF-B p65 after LPS stimulation were also determined by intracellular flow cytometry analysis. Briefly, 1620 hours before stimulation, 1.010⁶ cells of each cell line were seeded in 6-well plates in complete media containing 100 g/ml Zeocin. …

• Product #201 – LPS from Escherichia coli O111:B4

Macrophage isolation, culture and transfection:

Cells were transfected with poly(dA:dT) (2 ng/mL; Sigma), MDP (20 ug/mL; Invivogen), Salmonella thyphimurium flagellin (140 ng/mL; Invivogen) and Bacillus anthracis Lethal toxin and protective antigen (1 ug/mL; List Biological Laboratories).

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis