Bacterial Toxin Research Citations

Reagents:

…Pertussis toxin was purchased from List Biological Laboratories Inc. (Campbell, CA, USA). …

EAE induction and assessment:

Six- to eight-week-old male B6 mice were injected s.c. in the back with 50 µg of MOG₃₅₅₅ peptide emulsified in incomplete Freund’s adjuvant supplemented with heat-inactivated Mycobacterium tuberculosis H37RA. Pertussis toxin (200 ng per mouse) dissolved in PBS was administered i.v. on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction and Tissue Collection:

Female C57BL/6 mice (6-8 weeks old) were induced with EAE by immunization with 125 µg of MOG₃₅₅₅ peptide…Mice received two intravenous (i.v.) injections of 300 ng Bordetella pertussis toxin (List Biological Laboratories Inc., Campbell, CA) the day of immunization. An identical pertussis toxin injection was repeated 3 days later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… SEB signals or IL-17A-positive cells were counted per mm of basement membrane under high-powered field (HPF, 400) at three different sites in the tissue. Reagents and cell culture. SEB was obtained from List Biological Laboratories INC. (Campbell, CA, USA). …

Neuronal tracing:

For tracer injections into the sciatic nerve to evaluate nerve regeneration, rats were anesthetized at 5 weeks post-spinal nerve injury with 4% isofluorane and the sciatic nerves at mid-thigh region ipsilateral to spinal nerve injury were exposed under aseptic conditions. A 5 l solution of 0.5% CTB (Cholera Toxin B subunit, low salt; List Labs)…were injected into the sciatic nerve at 35 sites using a Hamilton syringe.

Immunohistochemistry:

Frozen sliced sections of DRG (10 m) and spinal nerve (10 m) were serially mounted. Selected sections (at least 120 m apart from each other if from the same DRG) were incubated with primary reagents 24 h at 4 C, and with the secondary antibody for 2 hrs at room temperature. Primary antisera were: polyclonal goat anti-CTB IgG (1:5,000; List, CA),…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt
• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

4-hydroxytamoxifen (4-HT), tamoxifen and DTX injections:

DTX injections were performed as previously described… 40 ng/g of DTX (List Laboratories) was delivered by i.p. injections to mice at P21. Two treatments, separated by 72 hr, were given. Animals (mice) were sacrificed 2 to 3 weeks after the first injection.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Enzymatic activity of BoNT/A light chain. The endopeptidase activity of urea-denatured BoNT/A LC was assayed using a full length peptide SNAP-25 and the SNAPtide peptide (FITC/DABCYL; List Biologicals, Campbell, CA) as substrates. …

• Product #521 – SNAPtide Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin

Immunohistochemistry:

Antibodies used in this study were as follows: …goat anti-CTB (List Laboratories)…

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Antibodies and Reagents:

… Pertussis toxin from LIST BIOLOGICAL LABORATORIES; …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Protein transfection:

… Bone marrowderived macrophages were primed with 10 ng/ml ultrapure LPS (List Biologicals) for 3 h to induce proIL-1b expression before protein transfection, using Profect-P1-lipidbased protein delivery reagent …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Experimental autoimmune encephalomyelitis induction:

Mice used in each experiment were 8-12-wk-old female littermates. Experimental autoimmune encephalomyelitis (EAE) was induced by s.c. injection of mice (ve per group) with 100 mg myelin oligodendrocyte protein (MOG) peptides emulsied in CFA (Chondrex). Mice were also injected i.p. with 200 mg pertussis toxin (List Biological Laboratories) on days 0, 1, and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)