Bacterial Toxin Research Citations

Materials:

The following variants of LPS were used in these experiments: Salmonella minnesota R595 (Re) from List Biological Laboratories, Inc., cat # 304; …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Lipopolysaccharide challenge:

Mice were intravenously injected at 0900h with 50 g/kg lipopolysaccharide (LPS) from Salmonella minnesota R595 (List Biological Laboratories Inc, Hornby, Canada) into the tail vein. Blood samples were collected after 30, 60, 90, and 120 minutes for cytokine analysis.

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Experimental autoimmune encephalomyelitis (EAE) induction:

EAE was induced in 812 week old male and female mice by subcutaneous injection into the flanks and base of tail of 125g of MOG_35-55 (MEVGWYRSPFSRVVHLYRNGK) or PLP_139-151 (HSLGKWLGHPDKF) (Auspep, Australia) emulsified in Complete Freunds Adjuvant containing 4mg/ml Mycobacterium tuberculosis H37Ra (Difco, Detroit, MI). Mice also received an intraperitoneal (i.p) injection of 400ng pertussis toxin (List Biological, Campbell, CA) on days 0 and 3 of induction. Assessments of EAE severity were performed daily according to a 9-point paraplegia scale with 0.5-point increments …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Autoimmune Encephalomyelitis induction:
 
2.2.2 Immunization of mice
 
Mice were immunized by a subcutaneous injection of 100µl of the prepared emulsion in
the ventral thorax, with an injection of 50µl in two sides in the chest (left and right side).
At the time of immunization and 2 days after, mice were injected intraperitoneally with
75ng/ml pertussis toxin (List Biologicals, Campbell, CA). 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Pertussis toxin (300 ng; PTX, List Laboratories, Campbell, CA) was injected intravenously on the day of MOG 35 – 55 immunization and two days later. The remaining six sham mice underwent the same immunization procedure with only IFA without MOG 35 – 55 . Mice were assessed daily for neurological disabilities using a standard clinical score [ 33 ] system: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials and methods – Reagents:

… LPS was purchased from List Biological Lab (Campbell, CA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

ELISAs for the avidity measurement of anti-DFS70 autoantibodies and anti-diphtheria toxoid antibodies:

The ELISAs were performed as previously described [13-16], with modifications. Microtiter wells (Medisorp; Nunc, Roskilde, Denmark) were coated with recombinant DFS70 [9] or diphtheria toxoid (DT; List Biological Laboratories, Inc., Campbell, CA) in PBS (1 and 2 g/ ml, respectively). …

• Product #151 – Diphtheria Toxoid, from Corynebacterium diphtheriae

Retrograde labelling of the neurons projecting to the rostral raphe pallidus:

CTb in the hypothalamus was detected with goat anti-CTb antiserum (1:1000, List Biological, Campbell, CA, USA), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Chemicals and reagents:

Chemicals and reagents were purchased from the following sources: …endotoxin-free PBS and phenol-extracted LPS (E. coli O111:B4) that is free of lipoprotein contamination (List Biological Laboratories); …

Cell isolation:

…Platelet activation was induced for the stated time at 37C with 100 ng/ml LPS with addition of 100 ng/ml each of human rCD14 and recombinant LPS binding protein (LPB).

Mouse thrombosis:

…Whole blood of mice, injected or not with actinomycin D, was isolated 90 min after injection and stimulated with LPS for 60 min before leukocytes were isolated, and their ability to synthesize IL-1b mRNA relative to constitutive GAPDH was assessed by RT-PCR.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

EAE:

… Pertussis toxin (List Biological Laboratories, Campbell, CA, USA; 200 ng ip) was given on days 0 and 2. Animals were assessed daily in a blinded fashion using a 10 point scale score (EAE disease score) and sacrificed and assessed electrophysiologically as soon as …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)