Bacterial Toxin Research Citations

…female C57BL/6 mice (68-weeks-old) were sensitized to peanut by administering on four occasions, 1 week apart, 3.75 mg of peanut butter from Kraft , which contains 1 mg peanut protein and 10 µg of the adjuvant cholera toxin (CT) (List Biological Laboratories) in …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Ex Vivo Exposure of Whole Blood to Stx-2:

Immediately after blood collection blood was incubated with 0.9% NaCl as a negative control and Shiga toxin 2 (List Biological Laboratories, Inc. USA) in two concentrations, 10 ng/mL (Stx-2 condition 1) and 0.1 ng/mL (Stx-2 condition 2). The samples were then co-incubated for 24 hours at 37C, 5% (v/v) CO₂) before preparation and analysis

List Labs has discontinued Product #162 (Lyophilized) and has replaced Product #162 with Product #162L (Liquid):

• Product #162L (Liquid) – Shiga Toxin 2 from Escherichia coli

Human cytokine antibody array assay: 

After separation by centrifugation, WBCs were washed twice with complete RPMI 1640 medium, and the cells were resuspended in RPMI 1640 supplemented with 10% fetal bovine serum and penicillin-streptomycin. WBCs (5 10⁶ to 10 10⁶ cells per ml) were treated with ET (25 pmol PA plus 25 pmol EF), LT (25 pmol PA plus 25 pmol LF), …

The following materials were used in this study. B. anthracis EF, LF, and PA were purchased from List Biological Laboratories (Campbell, CA).

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

Materials:

…Difficile toxin B (TcdB) was purchased from List Biologicals (Campbell, CA). …

Inhibition of RhoGTPases in hydrogels:

200 ng/ml difficile toxin B (-Rho,Rac,Cdc42, List Biologicals, Campbell, CA) was used to pharmacologically inhibit Rho, Rac and Cdc42, Rho was specifically inhibited using cell permeable 1 g/ml C3 transferase…

• Product #155 – Toxin B from Clostridium difficile

Biotinylation of toxin A:

Commercially available and lyophilized toxin A (List Biological Laboratories, 152C) was reconstituted to 0.1 mg/ml with water according to manufacturers instruction. …

• Product #152C – Toxin A from Clostridium difficile

Peanut Allergy and Peanut-Induced Anaphylaxis Models Gastrointestinal sensitization:

Mice were sensitized as previously described (Arias et al., 2011) with minor modifications. Specifically, mice were gavaged with 3.75 mg of Kraft All NaturalTM PN butter, containing roughly 1 mg of PN protein, along with 5 µg of cholera toxin (CT) (List Biological Laboratories, Campbell, California) once a week for 4 weeks or once a day for 10 days. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… 25 µl of an aqueous mixture containing 1mg/ml cholera toxin (CT) adjuvant (List Biological Labs, Campbell, CA) was applied to each ear and unif ormly spread with a small paint brush. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… After centrifugation at 10,000 rpm for 60 min at 4 °C, the supernatant was filter-sterilized using distilled and deionized water, and lyophilized at 70 °C. Cholera toxin (CT) was purchased from List Biological Laboratories, Inc. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… J774A.1 macrophage-like cells were cultured in DMEM supplemented with 10% FBS (Sigma) and 2 mM l-glutamine. Cells were incubated with 11000 ng/ml LPS (ultra pure from E. coli O111:B4, List Biological Labs) at 37 C in DMEM/10% FBS. …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

SH-SY5Y cell culture, and cell treatment with TLR agonists, with Ca^2 +/ionomycin, and with TLR pathways inhibitors:

… The TLR agonists used were: … lipopolysaccharide LPS (E. coli K12, D31m4(Re)) (List Biological Laboratories Inc., California, USA) (stock solution of 1 mg/ml) was added at a final concentration of 100 ng/ml; …

• Product #302 – LPS from Escherichia coli K12, D31m4 (Re)