Bacterial Toxin Research Citations

Cell Culture:

C3H10T1/2 cells (ATCC) were cultured in BME (Gibco) with 10% fetal bovine serum (PAA Laboratories) and penicillin-streptomycin. Cells were grown at 37C and 5% CO₂. For qPCR analysis, 5.010⁴ cells were plated in a 6-well dish. For protein extracts, 1.010⁶ cells were plated in a 15 cm plate. Where indicated, cells were treated with Shh (1 g/mL; R&D Systems) for 2 hours, 6 hours, or 24 hours. Where indicated, cells were treated for 24 hours with SAG (100 nM; Santa Cruz) or pertussis toxin (PTX; 100 ng/mL; List Biological Laboratories Inc.).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Slice preparation and electrophysiology (in rats):

In indicated experiments KCl was reduced to 4 mM or the following were added: 10 mM 1,2-Bis(2-aminophenoxy)ethane-N,N,N,N-tetraacetic acid (BAPTA; Sigma), 5gmL¹ Botulinum Neurotoxin Type C (Light Chain Recombinant BoNT/C; List Biological),

• Product #625A – Botulinum Neurotoxin Type C Light Chain, Recombinant

Slice Preparation and Electrophysiology (in mice):

…the light chain BoNT/C (the proteinase subunit which lacks membrane permeability, 5 g/ml, List Biological Laboratories) were included in the internal solution.

• Product #625A – Botulinum Neurotoxin Type C Light Chain, Recombinant

… A commercially available rLF used as control was purchased from List Biological Laboratories. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

… Animals were immunized via the intranasal route with 5 µg cholera toxin (CT; List Biological Laboratories, Campbell, CA, USA), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunization:

Once weekly for 3 consecutive weeks, female BALB/c mice were immunized intranasally with cCHP-PspA, PspA plus CT (1 µg; List Biological Laboratory, Campbell, CA), PspA alone, or phosphate-buffered saline (PBS) only. Some experiments included an irrelevant antigen as a control; …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Table 1. Antibody Characterization:

Cholera toxin subunit B, Purified CTB isolated from Vibrio cholerae, List Biological Laboratories (Campbell, CA), goat polyclonal #703, 1:5,000. …

Histology:

Table 1 lists all antibodies used. The CTB antibody was tested on galago brain tissue with no CTB injections and this control failed to label any cells or patches of axon terminals.

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Table 1. Antibody Characterization: 

…List Biological Laboratories (Campbell, CA), goat polyclonal #703, 1:5,000. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Neonatal LPS injections and hypoxic-ischemic brain injury:

All experiments were performed on C57/Bl6 mice…Mice were given a 0.3 mg/kg or 1.0 mg/kg single intraperitoneal injection (i.p.) of LPS (ultra pure E. coli 055:B5, List biological laboratories, Inc) at postnatal day 9…

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

Reagents:

…LPS (R595Re) and pertussis toxin were purchased from List biological. …

Results

Zymosan induces increases in cAMP and activation of PKA in wild type BMDMs:

…Activation of the TLR3 or TLR4 pathway with their respective ligands, poly I:C or LPS, did not acutely affect the intracellular concentration of cAMP (up to 15 minutes post ligand addition). …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).