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April 10, 2013
Neuron
McCoy, ES;Taylor-Blake, B;Street, SE;Pribisko, AL;Zheng, J;Zylka, MJ;
Product: Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
Experimental Procedures:
Animals – Cgrp-GFP/ female mice (McCoy et al., 2012) (available from MMRRC:36773) were crossed with male Advillin-Cre/ mice (Hasegawa et al., 2007) to generate double heterozygous CGRP+/; Advillin-Cre+/ (CGRP-DTR+/) mice. Heterozygous offspring were used for all experiments and have one functional Calca allele. All mice were backcrossed to C57BL/6 mice for at least eight generations.
Diphtheria toxin (DTX; List Biological Laboratories, Product 150) was dissolved in sterile 0.9% saline and stored at 20C in aliquots that were thawed once. DTX from List Biological was used for all (of the following) experiments…(1) Sensory neuron markers in lumbar DRG from CGRP-DTR+/- mice. (2) CGRP-IR afferents ablated in hairy skin. (3) Ablation of Mrgpra3-expressing (chloroquine receptor) DRG neurons in CGRP-DTR+/- mice. (4) DTX-treatment does not affect cold or heat sensitivity in wild-type mice or body weight.
April 9, 2013
Advances In Bioscience And Biotechnology
Koulchitsky, SV;Pashkevich, SG;Navasiolava, NM;Custaud, M;Kulchitsky, VA;
Product: LPS from Escherichia coli O111:B4
… 1.5 hours after the adaptation of animals to experimental conditions control measurements of recorded parameters were made (twice with 30 min interval). Injection of endotoxin (3 g/kg, lipopolysaccharide, LPS, Escherichia coli, 0111:B4, List Biological Laboratories, Campbell, CA; lot No LPS-25E) or apyrogenic saline (APS, Abbott Laboratories, North Chicago, IL 60,064; lot No 18-379-DK) was performed intraperitoneally of intravenously through silicone catheter in the amount not exceeding 0.5 ml. Tr monitoring was continuously carried out for at least 7 hours after the injection.
April 8, 2013
Current Protocols In Immunology
Myers, JM;Fairweather, D;Huber, SA;Cunningham, MW;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
… Pertussis toxin (PTX) stock Dissolve PTX (List Biological Laboratories) in sterile 0.1 M NaPO 4 /0.5 M NaCl, pH 7.0. Do not sterile filter the solution. Store in evaporation-resistant containers 6 to 12 months at 4C. The high ionic strength helps preserve PTX activity for long-term storage at 4C.
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
April 5, 2013
The Journal Of Biological Chemistry
Rubinow, KB;Wall, VZ;Nelson, J;Mar, D;Bomsztyk, K;Askari, B;Lai, MA;Smith, KD;Han, MS;Vivekanandan-Giri, A;Pennathur, S;Albert, CJ;Ford, DA;Davis, RJ;Bornfeldt, KE;
Product: ULTRA PURE LPS from Escherichia coli O111:B4
Bone Marrow-derived Macrophage (BMDM) Isolation and Culture:
… For stimulation/activation, media were changed on day 6 to low glucose DMEM (5.5 mm) with 1% non-essential amino acids (Invitrogen), 7% FBS, 30% L929 cell-conditioned medium, and antibiotics. BMDMs were variably stimulated with…ultrapure LPS (E. coli 0111:B4; List Biological Laboratories, Inc., Campbell, CA), …
April 5, 2013
Cell Death And Disease
Xu, J;Zhang, Y;Xiao, Y;Ma, S;Liu, Q;Dang, S;Jin, M;Shi, Y;Wan, B;Zhang, Y;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
Induction and treatment of EAE:
… Pertussis toxin (200 ng; List Biological Laboratories, Campbell, CA, USA) in phosphate-buffered saline (PBS) was administered iv on days 0 and 2. For preventive or treatment protocols of EAE, …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
April 4, 2013
PLoS ONE
Miura, K;Diakite, M;Diouf, A;Doumbia, S;Konate, D;Keita, AS;Moretz, SE;Tullo, G;Zhou, H;Lopera-Mesa, TM;Anderson, JM;Fairhurst, RM;Long, CA;
Product: Tetanus Toxoid from Clostridium tetani
Measurement of antigen-specific IgG levels by ELISA:
…To measure anti-tetanus toxoid IgG titers and determine IgG subclass, we randomly selected plasma samples from eight pairs of matched HbAA and HbAS children in each age group (35, 68 and 911 years) and tested them (n = 48) at a 1500 dilution. Tetanus toxoid was purchased from List Biological Laboratories, Inc. (Campbell, CA).
April 4, 2013
Anaerobe
Carlson, PE;Walk, ST;Bourgis, AE;Liu, MW;Kopliku, F;Lo, E;Young, VB;Aronoff, DM;Hanna, PC;
Product: Toxin B from Clostridium difficile
… Toxin activity in culture supernatants was quantified based on a standard curve, which was generated using known concentrations of purified C. difficile toxin B (List Biological Laboratories, Inc). Assays were performed in triplicate for each isolate tested. …
April 4, 2013
Frontiers in Neural Circuits
Hira, R;Ohkubo, F;Tanaka, YR;Masamizu, Y;Augustine, GJ;Kasai, H;Matsuzaki, M;
Product: Anti-Cholera Toxin B Subunit (Goat)
Fluorescent Tracing Using Cholera Toxin B Subunit:
…Sections were incubated with a goat anti- CTB antiserum (List Biological Laboratories,# 703, 1: 60000) in PBS-XD overnight at room temperature. …
April 3, 2013
Science Translational Medicine
Kurnellas, MP;Adams, CM;Sobel, RA;Steinman, L;Rothbard, JB;
Product: Pertussis Toxin from B. pertussis, Lyophilized in Buffer
Induction of EAE in mice by immunization with MOG and adjuvant and treatment with different hexamers:
EAE was induced by procedures… All mice were administered 400 ng of pertussis toxin (List Biological) intraperitoneal at 0 and 48 h post-immunization. …
Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)
April 2, 2013
PNAS
Benefield, DA;Dessain, SK;Shine, N;Ohi, MD;Lacy, DB;
Product: Botulinum Neurotoxin Type A from Clostridium botulinum
Specimen Preparation and EM:
The BoNT/A1 (Product #130), /B (Product #136), and /E (Product #141) PCs were purified by List Biological Laboratories and stored in lyophilized form.