Bacterial Toxin Research Citations

Murine models of ALI:

…Female C57BL/6 mice, age 8 12 wk, were treated with 20 g LPS (Escherichia coli 0111:B4; List Biological Laboratories) in 50 l saline or 50 l saline intratracheally (i.t.) and euthanized 2 8 days later. …

• Product #201 – LPS from Escherichia coli O111:B4

Peptide synthesis and immunization procedures:

… To measure recall responses, MOG 3555 emulsified in complete Freund’s adjuvant (CFA) was administered s.c., in the inguinal and sternal regions (100 g per mouse). Disease was induced with peptide emulsified in CFA supplemented with Mycobacterium tuberculosis H37RA extract (Difco Laboratories, Detroit, MI) to a final concentration of 5 mg/ml (200 g/mouse). Additionally, pertussis toxin (PT; List Biological Laboratories, Campbell, CA) was administered i.p., (200 ng/mouse) on day 0 and day 2 postimmunization (Massilamany et al., 2011c).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera toxin (List Biological Laboratories): 1 mg/ml dissolved in 18.2 M H 2 O, stored at 20°C. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cholera toxin immunisation:

TCR^/ mice were sublethally irradiated (500 rad) and reconstituted with B6 or Rorc^/ bone morrow. Before immunization with cholera toxin mice were deprived of food for 2 h and then given 0.25 ml of a solution containing eight parts HBSS and two parts 7.5% sodium bicarbonate by oral gavage (o.g.) to neutralize stomach acidity. After 30 min, mice were o.g. immunized with 25 µg cholera toxin (List Biological Laboratories, USA). Concentrations of antibodies specific for cholera toxin were determined by ELISA with cholera toxin (List Biological Laboratories) as the capture agent.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

…Subjects were anesthetized intramuscular (im) with a mixture of KetamineXylazine (355 mg/Kg) before receiving a unilateral intraocular 0.5% of CT-B (List Laboratories Inc., Campbell, CA) injection diluted in 0.1% dimetylsulphoxide (DMSO) into the posterior chamber of their …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

2.2. Bacterial CW Preparation and Anthrax Toxin:

An avirulent B. anthracis strain SNKE-FF-E308D was used for CW preparation. After growth, the CW was prepared according to Medvedevs protocol [17]. The amount of CW ranged between 30 and 35% of the dry weight. The CW preparations were checked for aerobic and anaerobic bacteria, endotoxin, and LPS contamination. Bacterial contamination was checked using various growth media. The endotoxin and LPS were tested using a Limulus amoebocyte lysate test kit (E-TOXATE, Sigma, St. Louis, MO, USA). No endotoxin and LPS were found in the preparations. PA, LF, and EF were purchased from List Biologicals Inc. (Campbell, CA, USA). The activity of LeTx was determined by its cytolytic effect on murine macrophage cells RAW264.7. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

… For mAb production and immunofluorescence analysis, pXO1 or pXO2 of B. anthracis H9401 was cured as described by Green et al. [21]. 2.2. Reagents. PA purified as described [22] was obtained from Green Cross Co. (Korea). LF was obtained from List Biological Laboratories. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

Assessment of serum anti-PA IgG and TNA antibody titers

Serum anti-PA IgG was detected by enzyme-linked immunosorbent assay (ELISA) as described previously,²⁰ Chichester JA, Musiychuk K, de la Rosa P, Horsey A, Stevenson N, Ugulava N, et al. Immunogenicity of a subunit vaccine against Bacillus anthracis. Vaccine 2007; 25:3111 – 4; http://dx.doi.org/10.1016/j.vaccine.2007.01.068; PMID: 17280756 [Crossref], [PubMed], [Web of Science ], [Google Scholar] and data was expressed as the geometric mean titer (GMT) standard error (SE) per group. Briefly, serum samples were titrated on plates coated with commercially available PA (List Biological Laboratories, Campbell, CA). …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Animals and vaccinations:

… For rabbit experiments, female New Zealand White (NZW) rabbits (Covance Research Products, Denver, PA) were immunized on day 0 with 250 g of the Rec-LND or with control PA83 (List Biological Laboratories, Inc., Campbell, CA) in an emulsion with CFA and were then boosted 4 times at 2-week intervals with 125 g of the Rec-LND or PA83 in an emulsion with incomplete Freund’s adjuvant (IFA). …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Toxin neutralization assay:

J774A.1 murine macrophages were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum and 1% Glutamax (Gibco). …Purified recombinant lethal toxin (LT), i.e., PA and lethal factor (LF), were purchased from List Biologicals (Campbell, CA). LT was reconstituted from equimolar amounts of PA and LF. Pooled serum from either mock-immunized (phosphate-buffered saline [PBS]) or anthrax vaccine adsorbed (AVA)-immunized mice was incubated with 0.1 g of LT for 20 min at room temperature. LT or LT-serum mix was transferred to J774A.1 cells. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169L (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis