Bacterial Toxin Research Citations

November 2, 2012

Plos One

Dittrich, AS;Winkler, T;Wellman, T;de Prost, N;Musch, G;Harris, RS;Vidal Melo, MF;

Product: LPS from Escherichia coli O55:B5

Experimental Protocol:

… After the baseline scans, five sheep received a continuous 10 ng/kg/min intravenous infusion of Escherichia coli endotoxin (lipopolysaccharide, LPS, O55:B5, List Biological Laboratories Inc, California) while six did not.

In this manner, four regional pulmonary pathophysiological conditions were studied:
1. healthy lung, i.e., no LPS and no lung lavage (LPS, Lav)
2. lung exposed to bronchoalveolar lavage but not to endotoxin (LPS, Lav+)
3. lung exposed to systemic endotoxin but not to lavage (LPS+, Lav)
4. lung exposed to systemic endotoxin and bronchoalveolar lavage (LPS+, Lav+)
NOTE: At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.
- Product #203A – LPS from Escherichia coli O55:B5

November 1, 2012

Annals Of Neurology

Nobuta, H;Ghiani, CA;Paez, PM;Spreuer, V;Dong, H;Korsak, RA;Manukyan, A;Li, J;Vinters, HV;Huang, EJ;Rowitch, DH;Sofroniew, MV;Campagnoni, AT;de Vellis, J;Waschek, JA;

Product: LPS from Escherichia coli O55:B5

In vivo LPS treatment in mice:

Experiments were performed according to protocols approved by the Chancellors Animal Research Committee of the Office for Protection of Research Subjects at the University of California, Los Angeles. LPS (Escherichia coli strain O55:B5, lot number 20316A1) was purchased from List Laboratories. We determined that the concentration of 10 mg/kg in this lot was adequate to induce the white matter injury in C57BL/6 mice as described in the results. LPS was diluted in sterile saline to a concentration of 0.5 mg/ml and injected subcutaneously between the shoulders. Please see supplementary materials for detailed information on injection.

NOTE: At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.
- Product #203A – LPS from Escherichia coli O55:B5

November 1, 2012

Clinical And Vaccine Immunology

Som, A;Navasa, N;Percher, A;Scott, RW;Tew, GN;Anguita, J;

Product: ULTRA PURE LPS from Salmonella minnesota R595 (Re)

Reagents, bacterial strains, and cells:

Ultrapurified LPS from Salmonella enterica subsp. enterica serovar Minnesota R595 (S. Minnesota) was from List Biological Laboratories, Inc. (Campbell, CA). …

Stimulation with TLR ligands and S. aureus:

The following general stimulation program was followed unless otherwise stated. RAW 264.7 cells (1 106) or BMDMs (see the supplemental material) were seeded in 12-well plates in 1 ml of complete RPMI medium. After 3 h, the cells were incubated with 1 g/ml SMAMP for 1 h and then stimulated with the following concentrations of TLR ligands: LTA (10 g/ml), LPS (100 ng/ml), poly(IC) (1.0 g/ml), …
- Product #434 – ULTRA PURE LPS from Salmonella minnesota R595 (Re)

November 1, 2012

Journal Of Immunology

Davis, MJ;Gregorka, B;Gestwicki, JE;Swanson, JA;

Product: LPS from Salmonella typhimurium

Materials and Methods:

… LPS no. 225 Salmonella typhimurium was from List Biological Laboratories (Campbell, CA), …

Immunouorescence microscopy:

BMM on 12-mm circular coverslips were stimulated overnight with IFN-g or LPS, then prepared for immunouorescence microscopy. Cells were xed for 60 min at 37C (xative: 2% paraformaldehyde, 4.5% sucrose, 20 mM HEPES, pH 7.4, 70 mM NaCl, 10 mM KCl, 10 mM MgCl2, 2 mM EGTA, 70 mM lysine-HCl, 10 mM sodium periodate), rinsed …
- Product #225 – LPS from Salmonella typhimurium

November 1, 2012

American Journal Of Physiology. Renal Physiology

Altuntas, CZ;Daneshgari, F;Izgi, K;Bicer, F;Ozer, A;Sakalar, C;Grimberg, KO;Sayin, I;Tuohy, VK;