Bacterial Toxin Research Citations

Immunohistochemical procedures:

Sections were incubated in the primary antibody solution, goat anti-CTb (1:20,000 in antibody dilution solution; List Biological Laboratories, lot #7032A6)

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Animals and DT treatment:

To ablate MCH neurons, male mice between 8 and 12 weeks of age were injected twice with DT [50 ng/g, intramuscular (i.m.); 2 d apart; List Biologicals]. DT was dissolved in saline (SAL; 0.9% NaCl).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Materials:

Botulinum neurotoxin type A toxoid and anti botulinum toxin type A chicken IgY were purchased from List Biological Laboratories (Campbell, California, USA).

Cutaneous delivery of botulinum toxoid A using liquid-loaded PMNs:

PMNs (n=3) were liquid-loaded, as previously described, with a solution containing formaldehyde inactivated BT i.e. botulinum toxoid (BTD) (100ng/l)…Next, slides were washed with PBS and incubated for 1 h with a diluted primary polyclonal antibody (Anti-botulinum toxin/toxoid type A chichen IgY).

Cutaneous delivery of fluorescence-conjugated BTD using liquid-loaded PMNs:

BTD was fluorescently labelled with PE/Cy7 using the EasyLink PE/Cy7 kit…The PMN was then re-imaged by fluorescence and light microscopy to evaluate release of the fluorescent BTD formulation from the pockets.

• Product #133L – Botulinum Neurotoxin Type A Toxoid from Clostridium botulinum
• Product #730A – Anti-Botulinum Neurotoxin, Type A (Chicken lgY)

… To assess the potential of DNI and DNI conjugate as a vaccine against B. anthracis, we investigated whether the vaccinations protect against an injection of LeTx. Three weeks after the last vaccination, each mouse was injected into the tail vein by a mixture of 48 g pa and 20 g LF (list biologicals, Campbell, CA). …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

EAE:

All mice used in EAE experiments were 58 weeks of age…mice received 150 ng of pertussis toxin (List Biological Laboratories) diluted in 100 µl PBS i.v. Over a period of 20 days, mice were monitored daily for clinical signs of EAE and disease was scored as follows: 0, no disease; 1, limp tail; 2, partial hind limb paralysis; 3, total hind limb paralysis; 4, forelimb paralysis; 5, moribund/dead.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… DTx List Biological Lab., Inc., California, USA …

MHC-II-antigenspezifische molecules (50% CD11c-DTR 50% MHC-II-/–chimeras):

For this purpose, the various groups were treated as described above, starting one day before immunization until the end of the EAE, every 48 hours with DTX or PBS and examined for clinical signs of disease. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

In Vivo OT-I Adoptive Transfer:

After 6 days, CFSE dilution on OT-I CD8⁺ T cells was analyzed. For DC depletion, Itgax-DTR-EGFP bone marrow chimera mice were treated i.p. with 100 ng diphtheria toxin (List Biological Laboratories, Inc., Campbell, CA) 1 day before and after circular antigen transfer.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

In vivo OT-I adoptive transfer:

For DC depletion, Itgax-DTR-EGFP bone marrow chimera mice were treated i.p. with 100ng diphtheria toxin (List Biological Laboratories, Inc., Campbell, CA) 1 day before and after circular antigen transfer.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Immunohistochemical procedures:

…For CTb immunostaining, the sections were incubated in goat CTb antiserum (List Biological Laboratories, Campbell, CA, USA) 1:20,000 in phosphate-buffer-saline with 0.3% Triton X-100 (PBST) and exposed to biotinylated donkey goat antiserum …

• Produt #703 – Anti-Cholera Toxin B Subunit (Goat)

Reagents:

… Pertussis toxin was obtained from List Biological Laboratories (Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)