Bacterial Toxin Research Citations

RNAi in Mouse Macrophage Cell Lines:

… Escherichia coli O111:B4 LPS was from List Biological Laboratories; …

Used in:

Identification of an Innate Immunity Protein Interaction Network

Modulate LPS-induced Cytokine Production in Mouse Macrophage Cell Lines

Sf3a1 and Golga4 Regulate LPS-induced Cytokine Secretion

• Product #201 – LPS from Escherichia coli O111:B4

…On the day of immunization as well as two days later, the mice were Intraperitoneal (I.P.) 400 ng Pertussis toxin (Ptx; List Biological laboratories). Within the experiments, the animals were examined daily for paralysis and the degree of disease or disability was determined by the following index (scoring):

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Chemicals:

… Ultra pure lipopolysaccharide (LPS, from Escherichia coli 055:B5) was obtained from List Biological Laboratories (Campbell, CA). …

Intestinal Organ Cultures:

Immature and mature human intestinal xenografts were exposed to LPS and IL-1 in organ culture (2022). Organ culture was performed as described previously (20). Quadruple organ cultures from each xenograft were challenged with 50 g/ml of ultra pure LPS, …

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

Induction of EAE, evaluation of clinical disease, and administration of peptides:

Female SJL/J mice (6 to 8 weeks old) were purchased from Jackson Laboratories (Bar Harbor, ME) and housed in standard SPF facilities. On day 1, SJL/J mice were injected with 300 µg/mouse bovine myelin basic protein (Invitrogen) emulsified in Complete Freunds Adjuvant with 8 mg/ml H37Ra Mycobacterium tuberculosis (Sigma-Aldrich) and injected subcutaneously into two sites at the base of the tail along with 400 ng/mouse pertussis toxin (List Biological Laboratories Inc) in PBS i.p. On day 3, the pertussis toxin injection was repeated (Jager et al., 2011). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and evaluation of disease:

Experimental autoimmune encephalomyelitis was induced in C57BL/6 wild type mice by s.c. injection over four sites in the flank with 200 µg/mouse of myelin oligodendrocyte glycoprotein peptide 3555 (C S Bio Co. Menlo Park, CA, USA) in an emulsion with Complete Freunds Adjuvant (Difco, Detroit, MI, USA). Pertussis toxin (List Biological Laboratories Inc., Campbell, CA, USA) dissolved in PBS was injected i.p. at 200 ng/mouse at the time of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… C57BL/6 mice were obtained from the Centre for Animal Resources (CARE) of the National University of Singapore, and CD137L-/- mice were a gift from Amgen and bred in-house under pathogen-free conditions. Mice were injected subcutaneously with 100 µg MOG35-55 (Sigma-Aldrich) and 1 mg heat-killed Mycobacterium tuberculosis H37RA (Difco) emulsified in complete reunds adjuvant. Pertussis toxin (200 ng in PBS; List Biological Laboratories) was injected intraperitoneally on days 0 and 2 after immunization. EAE clinical symptoms were scored daily as follows: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Cholera toxin (25 µg/mL stock; List Biological Laboratories, Campbell, CA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Liver Metastasis Model:

Tumor cells (5 10⁵/100 L phosphate-buffered saline [PBS]) were injected into the spleens of C57BL/6, CCR2 knockout (KO), severe combined immunodeficiency (SCID), or CD11b-diphtheria toxin receptor (DTR) mice. …CD11b-DTR or C57BL/6 mice received diphtheria toxin (7.5 ng DT/g body weight; List Biological Laboratories)…

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Materials:

Recombinant light chain, purified 150 kDa BoNT/A, and SNAPtide (FITC/DABCYL) were from List Biological Laboratories (Campbell, CA), …

The above materials were used in the following:

• PL63 and PL50 Kinetic Parameters
• Fluorescent and HPLC Analysis of Peptide Cleavage and Impact of Exosite-Binding Peptides
• Standard BoNT/A LC Toxin Detection Curves
• Limit of Detection and Quantitation Determinations
• Effect of TMAO on Peptide Substrates

• Product #610A – Botulinum Neurotoxin Type A Light Chain, Recombinant
• Product #521 – SNAPtide Peptide Substrate (FITC/DABCYL) for C. botulinum Type A Neurotoxin

…After BDA administration, another glass capillary (tip diameter: 30 m) filled with 0.5% CTB (List Biological Laboratories, Campbell, CA) was lowered vertically into either the occipital cortex, area 2, lateral part (Oc2L), Te2D, or the temporal cortex, area 1 (Te1) (anteroposterior …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt