Bacterial Toxin Research Citations

ELISAs:

… We tested coating the plates with the following Ags, all diluted in PBS: nicked diphtheria toxin (DT; 10 g/ml; List Biological Laboratories), DTaP vaccine (1:333, Sanofi Pasteur), NP 36 CGG (10 g/ml), and NP 16 CGG (10 g/ml), along with a PBS-only plate. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Cholera toxin B subunit:

(CTb; 1% solution in 0.1 M phosphate buffer; List Biological Laboratories, Inc., Campbell, CA) was used as an anterograde tracer to identify central projections of corneal afferents into the trigeminal dorsal horn (Todd et al., 2003; Hegarty et al., 2010). …The eye was dried again, the steel ring replaced around the abraded surface, and CTb (6-15 µl) was placed inside the ring for 30 min before being rinsed off with saline. Rats received a subcutaneous injection of ketoprofen (2.5 mg/kg) to reduce discomfort.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… C. difficile toxin B, purchased from List Biological Laboratories, Inc., (Campbell, CA) was added to the culture medium at a concentration of 0.25 g/ml for the indicated times. …

• Product #155 – Toxin B from Clostridium difficile

Phenotype MicroArray (PM) panels, chemicals, toxins, anti-toxins, bacterial strains, and mammalian cells lines:

…Clostridium difficile toxin A and toxin B were from List Biological Laboratory (Listlab, Campbell, CA, USA). …

Determination of toxin production by C. difficile and other Clostridium species:

…C. difficile toxins A and B from Listlab were used as standards to determine the relationship between toxin concentration and the corresponding level of inhibition of dye reduction rate by the indicator mammalian cells. …

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

…Four immunization protocols were tested comparing two adjuvants (poly I:C, Invivogen, San Diego, CA and cholera toxin, List Biological Laboratories, Campbell, CA) and 2 administration schedules. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Animals and EAE/EAU Induction:

…For EAE induction, mice were injected subcutaneously with 100 µg MOG₃₅₅₅ peptide (synthesized by Toray Research Center, Tokyo, Japan) and 1 mg heat-killed mycobacterium tuberculosis H37RA emulsified in complete Freunds adjuvant (Difco, KS, USA). 200 ng Pertussis toxin (List Biological Laboratories, USA) was injected intraperitoneally (i.p.) on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (PTx ) List Biologicals, USA …

Induction of active EAE:

Active EAE in C57BL/6 mice was induced by subcutaneous immunisation with 250 µg MOG35-55-peptide and 800 g H37RA emulsified in CFA, followed by two intraperitoneal doses of 400 ng PTX in PBS at the time of immunisation and 48 hours later. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In vivo experiments:

…together with Complete Freund’s Adjuvant and heat-killed Mycobacterium tuberculosis (H37Ra, Difco). Bordetella pertussis toxin (250ng; List Biological Laboratories, US) was administered intraperitoneally at days 0 and 2. On each day following immunization mice were weighed and scored as follows: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

MOG-induced EAE: active induction of EAE:

C57Bl6/J WT control mice and AKT32/2 mice were immunized with MOG peptide at 8 wk of age. …In addition, 200 ml pertussis toxin (2.5 mg/ml; List Biological Laboratories, Campbell, CA) was injected into the tail vein on days 0 and 2. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Trpm8^DTR mice and TRPM8 neuron ablation:

Two consecutive diphtheria toxin (DTx) intraperitoneal injections (50 g/kg in normal saline; List Biological Laboratories;…)were administered 3 d apart to both transgenic mice and their control littermates aged 8 weeks.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae