Bacterial Toxin Research Citations

… PA and LF used for the toxin neutralization assay were purchased from List Biological Laboratories. …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

MATERIALS AND METHODS

Toxins:

TcdA and TcdB were purchased from List Biological Laboratories, Inc. (Campbell, CA) and tgcBIOMICS GmbH (Mainz, Germany). List Biological Laboratories toxins were purchased in vials containing 20 to 25 g of lyophilized protein and stored at 4C. Toxins were reconstituted on the day of the assay to ensure maximum activity. tgcBIOMICS toxins were stored at 20C, and fresh aliquots were thawed as needed. Here, List Biological Laboratories is referred to as supplier 1, and tgcBIOMICS is referred to as supplier 2.

C. difficile toxoid vaccine preparation:

Lyophilized C. difficile TcdA and TcdB from supplier 1 (selected due to lower cost and availability of material) were each reconstituted and then buffer exchanged by dialysis into morpholinepropanesulfonic acid (MOPS)-buffered saline (for monkey immunization) or into 20 mM HEPES-buffered saline (pH 7.4) (for hamster immunization) to obtain final concentrations of 0.3 to 0.4 mg/ml based on the toxin quantity measured by the manufacturer. The toxins were converted to the toxoid state by addition of formaldehyde to 0.43% and lysine to 4.2 mg/ml and incubation for 16 to 18 days at 2C to 8C.

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

Peanut Sensitization Protocol and Intraperitoneal Challenge:

Female C57BL/6 mice (6-8 weeks old) were sensitized to peanuts by oral gavage treatment weekly with 1 mg peanut protein from Kraft peanut butter and 10 µg cholera toxin (List Biological Laboratories) in 100 µL of sterile water. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Vaccines and immunization schedule:

The vaccine was prepared by mixing 1 µg cholera toxin (CT) (List Biological Laboratories, Inc., Campbell, CA) with 20 µg endotoxin-free OVA (Hyglos, Germany) in a total volume of 12 µl PBS per mouse (6 µl per nare). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Immortalized human corneal epithelial cells (RIKEN Cell Bank, Tsukuba Science City, Japan) were cultured in DMEM/Ham’s F12 (1:1) supplemented with 10% FBS, 5 µg/ml insulin (Sigma), 0.1 µg/ml cholera toxin (List Biological Laboratories, Campbell, CA, USA), 0.5% DMSO …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Administration of TLR Ligands:

Postnatal day (PND) 8 mice were injected intraperitoneally (i.p.; 10 l/g body weight) … a TLR-3 ligand; LPS (List Biological Laboratories, Inc., ultrapure #423; 0.3 mg/kg), a TLR-4 ligand; … and were then deeply anesthetized and sacrificed 14 h postinjection for tissue sampling. …

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

Reagents:

Ultra Pure LPS (Escherichia coli 0111:B4) was obtained from List Biologicals (Campbell, CA). …

In vivo LPS-induced acute lung inflammation:

Mice were anesthetized using a mixture of isoflurane and oxygen bled in at 3.5L/min. LPS (List Biologicals, Inc., Campbell, CA) dissolved in DNase/RNase free distilled water (1mg/ml) was instilled intratracheally at a concentration of 1.5 mg/kg by direct laryngoscopy.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Adoptive transfer:

Before adoptive transfer, cultured cells were harvested and passed through 40 m cell strainers. To attenuate EAM, animals received intravenously 10⁶ CD133⁺ progenitors or CD133-derived dendritic cells at day 7 after MyHC/CFA immunization. For EAM induction, CD133⁺ progenitors, CD133-derived dendritic cells or bmDCs were pulsed with 10 g/mL MyHC peptide, activated with 0.1 g/mL lipopolysaccharide (LPS; List Labs) and 5 g/mL anti-CD40 prior to intraperitoneal injection of 5  10⁵ cells/mouse at days 0, 2 and 4.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Materials:

… LPS was obtained from List Biological Laboratories, Inc. …

LPS was used in the following applications:

• Cell culture
• Western blot analysis
• Cell adhesion assay

• Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Reagents and Plasmids:

… Purified lipopolysaccharide (LPS) from Escherichia coli was obtained from List Biological Laboratories Inc. (Campbell, CA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/