Bacterial Toxin Research Citations

Vaccines and immunization schedule:

The vaccine was prepared by mixing 1 µg cholera toxin (CT) (List Biological Laboratories, Inc., Campbell, CA) with 20 µg endotoxin-free OVA (Hyglos, Germany) in a total volume of 12 µl PBS per mouse (6 µl per nare). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Immortalized human corneal epithelial cells (RIKEN Cell Bank, Tsukuba Science City, Japan) were cultured in DMEM/Ham’s F12 (1:1) supplemented with 10% FBS, 5 µg/ml insulin (Sigma), 0.1 µg/ml cholera toxin (List Biological Laboratories, Campbell, CA, USA), 0.5% DMSO …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Administration of TLR Ligands:

Postnatal day (PND) 8 mice were injected intraperitoneally (i.p.; 10 l/g body weight) … a TLR-3 ligand; LPS (List Biological Laboratories, Inc., ultrapure #423; 0.3 mg/kg), a TLR-4 ligand; … and were then deeply anesthetized and sacrificed 14 h postinjection for tissue sampling. …

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

Reagents:

Ultra Pure LPS (Escherichia coli 0111:B4) was obtained from List Biologicals (Campbell, CA). …

In vivo LPS-induced acute lung inflammation:

Mice were anesthetized using a mixture of isoflurane and oxygen bled in at 3.5L/min. LPS (List Biologicals, Inc., Campbell, CA) dissolved in DNase/RNase free distilled water (1mg/ml) was instilled intratracheally at a concentration of 1.5 mg/kg by direct laryngoscopy.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Adoptive transfer:

Before adoptive transfer, cultured cells were harvested and passed through 40 m cell strainers. To attenuate EAM, animals received intravenously 10⁶ CD133⁺ progenitors or CD133-derived dendritic cells at day 7 after MyHC/CFA immunization. For EAM induction, CD133⁺ progenitors, CD133-derived dendritic cells or bmDCs were pulsed with 10 g/mL MyHC peptide, activated with 0.1 g/mL lipopolysaccharide (LPS; List Labs) and 5 g/mL anti-CD40 prior to intraperitoneal injection of 5  10⁵ cells/mouse at days 0, 2 and 4.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Materials:

… LPS was obtained from List Biological Laboratories, Inc. …

LPS was used in the following applications:

• Cell culture
• Western blot analysis
• Cell adhesion assay

• Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Reagents and Plasmids:

… Purified lipopolysaccharide (LPS) from Escherichia coli was obtained from List Biological Laboratories Inc. (Campbell, CA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Reagents and plasmids:

… Purified LPS from Escherichia coli was obtained from List Biological Laboratory Inc. (Campbell, CA, USA) and dissolved in endotoxin-free water. 

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

In vitro transduction and LPS stimulation:

In experiments quantifying NF-B signaling, Raw 264.7 cells were incubated with 100 ng/ml LPS (List Biological Laboratories, Campbell, CA) for 6 hours before analysis.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE:

… Each animal also received 300 ng pertussis toxin (List Biological Laboratories, Inc., Campbell, CA, Cat. no. 181) on days 0 and 1 post-immunization. Mice were randomly divided …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)