Bacterial Toxin Research Citations

Phenotype MicroArray (PM) panels, chemicals, toxins, anti-toxins, bacterial strains, and mammalian cells lines:

…Clostridium difficile toxin A and toxin B were from List Biological Laboratory (Listlab, Campbell, CA, USA). …

Determination of toxin production by C. difficile and other Clostridium species:

…C. difficile toxins A and B from Listlab were used as standards to determine the relationship between toxin concentration and the corresponding level of inhibition of dye reduction rate by the indicator mammalian cells. …

• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile

…Four immunization protocols were tested comparing two adjuvants (poly I:C, Invivogen, San Diego, CA and cholera toxin, List Biological Laboratories, Campbell, CA) and 2 administration schedules. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Animals and EAE/EAU Induction:

…For EAE induction, mice were injected subcutaneously with 100 µg MOG₃₅₅₅ peptide (synthesized by Toray Research Center, Tokyo, Japan) and 1 mg heat-killed mycobacterium tuberculosis H37RA emulsified in complete Freunds adjuvant (Difco, KS, USA). 200 ng Pertussis toxin (List Biological Laboratories, USA) was injected intraperitoneally (i.p.) on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (PTx ) List Biologicals, USA …

Induction of active EAE:

Active EAE in C57BL/6 mice was induced by subcutaneous immunisation with 250 µg MOG35-55-peptide and 800 g H37RA emulsified in CFA, followed by two intraperitoneal doses of 400 ng PTX in PBS at the time of immunisation and 48 hours later. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In vivo experiments:

…together with Complete Freund’s Adjuvant and heat-killed Mycobacterium tuberculosis (H37Ra, Difco). Bordetella pertussis toxin (250ng; List Biological Laboratories, US) was administered intraperitoneally at days 0 and 2. On each day following immunization mice were weighed and scored as follows: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

MOG-induced EAE: active induction of EAE:

C57Bl6/J WT control mice and AKT32/2 mice were immunized with MOG peptide at 8 wk of age. …In addition, 200 ml pertussis toxin (2.5 mg/ml; List Biological Laboratories, Campbell, CA) was injected into the tail vein on days 0 and 2. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Trpm8^DTR mice and TRPM8 neuron ablation:

Two consecutive diphtheria toxin (DTx) intraperitoneal injections (50 g/kg in normal saline; List Biological Laboratories;…)were administered 3 d apart to both transgenic mice and their control littermates aged 8 weeks.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

TLR stimulation and ELISA:

Macrophages or DCs were plated onto 96-well tissue culture-treated plates at 5 10⁴ cells/well and allowed to rest for 16 h prior to stimulation. …TLR agonists used were as follows: S. Minnesota R595 Ultra Pure LPS (List Biological Laboratories), …

Flow cytometry and intracellular cytokine staining:

Macrophages were stimulated with LPS for up to 8 h in the presence of 10 g/mL Brefeldin A for the final 2 h of stimulation. …

LPS injection:

Mice were injected with 1 g/g LPS i.p. in PBS and blood was collected at indicated time points. Serum concentrations of IL-12 p40, IL-6, TNF, and IL-10 were determined by ELISA.

Gene expression analysis:

Total RNA from LPS or TNF (Peprotech) stimulated macrophages was isolated using the RNeasy Plus kit (Qiagen) and reverse transcribed with…

ChIP:

ChIP was conducted as described in [35] with minor variations. Briefly, macrophages were stimulated with 1 ng/mL LPS for 8 h, washed and fixed with a 1% final concentration of formaldehyde…

• Product #434 – ULTRA PURE LPS from Salmonella minnesotaR595 (Re)

Materials:

Pertussis toxin (PTx) was from List Biological Laboratories (Campbell, CA).

Results:

…To determine whether the prolonged B2R-mediated NO response in cytokine-treated cells was dependent on coupling through G_i/o, we measured NO production from control and cytokine-treated HLMVEC pretreated with 1.5 µg/ml PTx for 3 h prior to stimulation with BK. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

… pertussis toxin (List Biological laboratories, Campbell …

Macrophage Culture and Phagocytosis of A:

PBMCs were isolated by Ficoll-Hypaque gradient technique from venous blood and were differentiated into macrophages (714 days) in IMDM medium (Thermo) with 10% autologous serum as described [11] [12]. Replicate AD macrophage cultures were pre-treated with either U0126 (72 nM), Wortmannin (500 nM), PKI (500 nM), PTX (1 µg/ml), EGTA, MK (10⁷M), anti-GPR32 (Chr32, 1/200), anti-PDIA3 (1/400), or …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)