Bacterial Toxin Research Citations

Induction of EAE in DR2-Tg:

… HLA-DR2-positive male and female mice between 8 and 12 weeks of age were immunized s.c. at four sites on the flanks with 0.2 mL of an emulsion of 200 g immunogenic peptide and complete Freund’s adjuvant… In addition, mice were given pertussis toxin (Ptx) from List Biological Laboratories (Campbell, CA, USA) on days 0 and 2 post immunization (75 and 200 ng per mouse, respectively). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunization:

… To induce development of EAE, the MOG-peptideimmunized B6 mice were also injected twice (on the day of immunization and 2 days later) with 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA) in PBS.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

EAE was induced by s.c. immunization of mice into the flanks with an emulsion of MOG3555 peptide (100 mg) emulsified in CFA supplemented with 4 mg/ml Mycobacterium tuberculosis extract H37Ra (Difco). Additionally, the animals received 200 ng pertussis toxin (List Biological Laboratories) i.p. on days 0 and 2. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

On Days 0 and 2, animals received ip injections of 250 ng pertussis toxin (List Biologicals.)…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Acute Lung Injury:

… BAL fluids were cleared of cells by centrifugation. All samples were stored at 80C until further analysis. Diphtheria toxin for depletion of CD11c⁺ cells in CD11c-DTR Tg mice was from List Biological Laboratories (Campbell, CA).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Retrograde spinal tracers used were … cholera toxin B subunit (CT-B; 1% in deionized water, List Biological Laboratories, Inc., Campbell, CA), …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Materials:

… Pertussis toxin (PTX) was purchased from List Biological Laboratories (Campbell, CA). …

Measurement of intracellular Ca²⁺ transient by FLIPR:

Jurkat T-cells were serum-starved overnight in the absence or presence of PTX (10 ng/ml) and then washed with Hanks balanced salt solution (HBSS). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Retrograde tracing:

To identify presympathetic RVLM-projecting PVN neurons (PVNRVLM), rats were anesthetized (ketamine-xylazine mixture, 90 and 50 mg kg-1, respectively, i.p.) and a stereotaxic apparatus was used to pressure inject 500 nl of rhodamine-labeled microspheres (Lumaflor, Naples, FL, USA) or cholera toxin B (CTB) (1%, List Biological Laboratories) into the RVLM (starting from Bregma: 12 mm caudal along the lamina, 2 mm medial lateral, and 8 mm ventral). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Immunization protocols:

…The anesthetized mice were placed on their backs, held at a downward angle then immunized with MOMP (100 µg) and either CTA1-DD (MIVAC, The University of Gteborg) (20 µg) or CT (List Biological Laboratories Sapphire Biosciences, NSW, Australia) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Oral immunization:

Thirty minutes later, the mice were orally immunized with 1mg OVA (Sigma-Aldrich) and 10µg CT (List Biological Laboratories, Campbell, CA). This procedure was conducted on days 0, 7 and 14…One week after the final immunization, faecal samples and mononuclear cells from the iLP were collected for enumeration of OVA-specific antibody responses by enzyme-linked immunosorbent assay and ELISPOT, respectively¹³. In vivo CT challenge was performed by oral challenge of naive or immunized mice with 100µg of CT as previously described⁴⁶.

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae