Bacterial Toxin Research Citations

… Each mouse also received an intraperitoneal injection of Pertussis toxin (PTX) (List Biological Labs, Campbell, CA) (150 ng) in 0.9% sterile buffered saline on day 0 and 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE in DR2-Tg and MBP-TCR/DR2-Tg mice:

…In addition, mice were given pertussis toxin (Ptx) from List Biological Laboratories (Campbell, CA) on days 0 and 2 post-immunization (75 ng and 200 ng per mouse, respectively).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of Cholangitis.

…injected intraperitoneally (IP) into 6 week-old female B6 mice. Additionally, mice received 100 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) in 100 µL of PBS (IP) at the time of, and 2 days after, the initial immunization with 2OA-BSA.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

Animals were immunized s.c. in the abdominal flank with 200 µl myelin oligodendrocyte glycoprotein peptide (MOG3555) (UCLA Biopolymers Core) emulsified in complete Freunds adjuvant (Sigma-Aldrich), followed by i.p. injection of 200 µl pertussis toxin (List Biological Laboratories) on days 0 and 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

B6 mice were immunized s.c. (into the flanks) with 100 µg MOG35-55p…Animals received 200 ng pertussis toxin i.v. (List Biological Laboratories) on days 0 and 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Depletion of TAMs in Braf-induced thyroid cancer mouse models:

Macrophage depletion in the bone marrow (BM), blood, spleen and peritoneal cavity was examined in Ccr2-DTR mice after treatment with diphtheria toxin (DT) 20 ng/g (List Biologicals, Campbell, CA ) intraperitoneally (i.p.) on alternating days for 7 days. Twenty-four hours following the last dose of DT, mice were euthanized by CO2 inhalation and tissue samples obtained for flow cytometry and/or immunohistochemistry (IHC) as described below.

To assess the effects of TAMs on PTC development, Tg-rtTA/tetO-Braf/Ccr2-DTR mice were fed dox-impregnated chow (2500 ppm; Harlan-Teklad) for 7 days with or without DT i.p. on alternating days beginning on day 0. On day 7 (24 hr after the last dose of DT), mice were euthanized by CO₂ inhalation and thyroids extracted for IHC. To examine the role of TAMs in established BRAF-induced thyroid cancers we treated Tg-Braf/Ccr2-DTR mice at approximately 6 and 12 weeks of age with or without DT on alternating days for 10 days. Mice were euthanized and thyroids extracted 24 hr after the last dose of DT for flow cytometry and IHC.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Materials:

…Staphylococcal enterotoxin B (SEB) was purchased at List Biological Laboratories (Campbell, CA),…

LC-MS:

… To avoid undesirable oxidation of cysteines by air oxygenation, digested SEB samples were further treated with 10 mM TCEP [tris(2-carboxyethyl)phosphine] and then with 100 mM iodoacetamide…

Immunohistochemical procedures:

Sections were incubated in the primary antibody solution, goat anti-CTb (1:20,000 in antibody dilution solution; List Biological Laboratories, lot #7032A6)

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Animals and DT treatment:

To ablate MCH neurons, male mice between 8 and 12 weeks of age were injected twice with DT [50 ng/g, intramuscular (i.m.); 2 d apart; List Biologicals]. DT was dissolved in saline (SAL; 0.9% NaCl).

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Materials:

Botulinum neurotoxin type A toxoid and anti botulinum toxin type A chicken IgY were purchased from List Biological Laboratories (Campbell, California, USA).

Cutaneous delivery of botulinum toxoid A using liquid-loaded PMNs:

PMNs (n=3) were liquid-loaded, as previously described, with a solution containing formaldehyde inactivated BT i.e. botulinum toxoid (BTD) (100ng/l)…Next, slides were washed with PBS and incubated for 1 h with a diluted primary polyclonal antibody (Anti-botulinum toxin/toxoid type A chichen IgY).

Cutaneous delivery of fluorescence-conjugated BTD using liquid-loaded PMNs:

BTD was fluorescently labelled with PE/Cy7 using the EasyLink PE/Cy7 kit…The PMN was then re-imaged by fluorescence and light microscopy to evaluate release of the fluorescent BTD formulation from the pockets.

• Product #133L – Botulinum Neurotoxin Type A Toxoid from Clostridium botulinum
• Product #730A – Anti-Botulinum Neurotoxin, Type A (Chicken lgY)