Citations

Bacterial Superantigens and Endotoxin:

… Lipopolysaccharide (LPS; Escherichia coli 055:B5) was purchased from List Biological Laboratories, Inc. (Campbell, CA) and reconstituted with PBS prior to use. …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

Induction and evaluation of EAU:

SLAT/Def6 KO mice and their C57BL/6 controls were immunized with bovine IRBP (150 µg), emulsified in complete Freunds adjuvant (CFA), administered subcutaneously [10]. In addition, the mice were injected intraperitoneally with 0.2 µg pertussis toxin (List Biological Laboratories, Inc., Campbell, CA). The development of ocular inflammation was determined by fundoscopy on day 12 post-immunization and by histological examination on day 14, following euthanization. Severity of disease, on a scale of 04, in half point increments, was scored as detailed elsewhere [11,12].

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Reagents:

… Ultrapure lipopolysaccharide (LPS) (Escherichia coli 0111:B4, TLR4 lig- and) was purchased from List Biological Laboratories, Inc. (Vandell Way, CA, USA). …

Results – DNA Stimulates Factor B Synthesis and Release by Macrophages:

Early upregulation of factor B occurs after ischemic tissue injury. However, the endogenous molecules that stimulate factor B production in the setting of ischemic tissue injury have not been identified. We have previously shown that microbial ligands, including LPS and polyinosinepolycytidylic acid (poly I:C) stimulate dynamic synthesis and release of factor B by macrophages (26). We next sought to test the hypothesis that one of several known endogenous mediators of inflammation known to activate patternrecognition receptors would stimulate factor B synthesis. Because we previously found that activation of TLR4 signaling by LPS resulted in synthesis of factor B in macrophages, we therefore studied the effect of several endogenous molecules that have been identified as activators of TLR4 signaling (3335). As previously observed, stimulation of RAW264.7 macrophages with LPS resulted in upregulation of factor B mRNA (9.9-fold, p<0. 001). However, no significant upregulation of factor B was observed after stimulation with fibrinogen, heparan sulfate or HMGB1 at known stimulatory concentrations (Figure 1). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

EAE induction:

… CD137L^-/- mice were a gift from Amgen and bred in-house under pathogen-free conditions. Mice were injected subcutaneously with 100 µg of myelin oligodendrocyte glycoprotein peptide fragment 35-55 (MOG_35-55) (Sigma-Aldrich) and 1 mg heat-killed Mycobacterium tuberculosis H37RA (Difco) emulsified in complete Freunds adjuvant. Pertussis toxin (200 ng in PBS; List Biological Laboratories) was injected intraperitoneally on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… cholera toxin was from List Laboratories; …

Intragastric antigen sensitization and treatment:

Sensitization was performed by 20 [micro]g OVA gavage with 10 [micro]g cholera toxin in 0.2 ml saline for 5 times once a week using a stainless steel blunt feeding needle. Mice in the non-sensitized control group received saline. Experimental diet included 0.1% or 0.3% red ginseng or fermented red ginseng. Mice were fed the experimental diet for 8 weeks starting at 1 week before the initial sensitization. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE induction and clinical evaluation:

EAE was induced by s.c. immunization with 100 mg MOG3555 peptide in 100 ml CFA containing 0.4 mg Mycobacterium tuberculosis H37RA (Difco); 200 ng pertussis toxin (List Biological Laboratories) was administered i.v. on days 0 and 2. Clinical scoring was: 1 = limp tail; 2 = hind limb paresis or partial paralysis; 3 = total hind limb paralysis; 4 = hind limb paralysis and body or front limb paresis or paralysis; 5 = moribund.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis:

Experimental autoimmune encephalomyelitis was induced in 4-mo-old male B6 or B6.Sle1c2 mice. On day 0, mice received an emulsion of 50 mg myelin oligodendrocyte glycoprotein peptide sequence 3555 and 500 mg Mycobacterium tuberculosis (Difco) in IFA (Sigma-Aldrich) via s.c. injections at the base of the tail. In addition, 500 ng pertussis toxin (List Biologicals) was administered i.p. on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Material:

… Pertussis Toxin (Catalog #179A; List Biologicals, Campbell, CA). …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (salt-free; List Biological Laboratories, Campbell, CA). It is sold as a lyophilized powder. Reconstitute with PBS to a final concentration of 2 g/ml and store at 4 C. 10. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Induction and Assessment of EAE:

For the induction of EAE, mice were immunized subcutaneously in the four flanks with 100 µg of MOG(3555) emulsified 1:1 in complete Freund’s adjuvant (Difco) supplemented with 500 µg of Mycobacterium tuberculosis H37Ra on day 0. In addition, 120 ng of pertussis toxin (PTX; List Biological Laboratories, Campbell, CA) was injected intravenously on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)