Citations

… based on their ultrastructural level, we injected CTB (List Biological Laboratories, CO, USA) into the TA muscle. Retrograde labeling was observed at the light microscope …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

2.2 Active experimental autoimmune encephalomyelitis induction and control immunization

aEAE was induced in both male and female mice by injection of myelin oligodendrocyte glycoprotein peptide 35–55 (MOG35-55 peptide, 200 μg per animal, Genscript) and complete Freund’s adjuvant (Incomplete Freund’s Adjuvant, Santa Cruz Biotechnology; supplemented with Mycobacterium tubercolosis, Difco). An emulsion of MOG35-55 and CFA was injected subcutaneously in mouse flanks and at the tail base at day 0; in addition, 300 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) was injected intraperitoneally at day 0 and day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

TLR agonists were: … Monophosphoryl Lipid A (MPLA) (List Biologicals, Campbell, California), …

• Product #401 – LIPID A monophosphoryl from Salmonella minnesota R595

Pertussis Toxin List Biologic Cat# 180

Induction and evaluation of EAE

To induce EAE, eight- to 12-week-old mice were injected subcutaneously with an emulsion contains 200 μg MOG35-55 in complete Freund’s adjuvant (CFA) containing 500 μg of nonviable, desiccated Mycobacterium tuberculosis at a concentration of 10 mg/mL on upper and lower back. Mice were i.p. injected with 200 ng pertussis toxin (PT) 2 hours later, and then repeated 2 days after immunization. For adoptive transfer of EAE with myelin-reactive T cells, splenocytes were isolated from MOG TCR transgenic (2D2) or wild type EAE mice. After removal of red blood cells by ammonium-chloride-potassium (ACK) lysis buffer, splenocytes were collected and suspended in RPMI-1640 medium containing 10% FBS. T cells were stimulated with 10 μg/ml MOG35−55 peptide for 48 h, and then expanded in 5 ng/ml IL-2 and IL-7 for 5 days. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Pertussis Toxin from List Biological Laboratories was dissolved in water …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Table 1Summary of primary antibodies and dilutions for the immunolabeling. IF: immunofluorescence. IHC: immunohistochemistry (peroxidase).

Antibody Host Antigen Manufacturer RRID Dilution
CTB Goat/Polyclonal Cholera Toxin B List Biological Lab., CA, USA AB_10013220 1:5000 (IF)

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… All animals were kept on a 12h dark-light cycle and standard housing conditions with ad libitum food access. At postnatal day 5 (P5), C57Bl/6 mice were randomly assigned to 4 experimental groups: lipopolysaccharides (LPS) (1 mg/kg, #423, List Biological Laboratories, Campbell, CA, USA)-injected mice …

• Product #423 – ULTRA PURE LPS from Escherichia coli O55:B5

… For immunolabeling, sections were blocked with 10% horse serum (Thermo Fisher Scientific, Waltham, MA, USA) in 0.1 M TBS, followed by the overnight incubation with primary antibody against choline acetyltransferase (ChAT, goat, 1:200, AB144P; Sigma-Aldrich, St. Louis, MO, USA) or CTB (goat, 1:500, 703; List Biological Laboratories, Inc., Campbell, NJ, USA) at 4 °C. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

2.4. EAE Induction and Scoring

Eight-week-old mice were immunized subcutaneously on the back with two injections of 150 μg myelin oligodendrocyte glycoprotein peptide (MOG35–55, GenScript, Piscataway, NJ, USA) emulsified in complete Freund’s adjuvant (CFA; Difco Laboratories, Franklin Lakes, NJ, USA) containing 2.5 mg/mL heat-killed Mycobacterium tuberculosis. Mice were further injected intraperitoneally with 200 ng pertussis toxin (List Biological, Campbell, CA, USA) in 0.1 mL of PBS at 0 and 48 h postimmunization with MOG35–55. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE

EAE was induced with 50 μg of myelin oligodendrocyte glycoprotein fragment MEVGWYRSPFSRVVHLYRNGK (MOG35–55 peptide; #crb1000205n Cambridge Research Biochemicals) mixed with incomplete Freund’s Adjuvant (#263910, BD) containing 4 mg/ml Mycobacterium tuberculosis TB H37 Ra (#231141, BD), at a ratio of 1:1 (v/v). Mice received two subcutaneous injections of 100 μl each of the MOG/CFA mix. Mice received a single intraperitoneal injection of pertussis toxin (#180, List Biological Laboratories) at a concentration of 1 ng/μL in 200 μL of PBS. A second injection of pertussis toxin at the same concentration was inoculated two days after the EAE induction. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer