Bacterial Toxin Research Citations

3.3.3) Experimental autoimmune encephalomyelitis (EAE) induction

Young (8-week) and middle-aged (52-week) female C56BL6 mice (Jackson Laboratories) were subcutaneously injected with 50µg per 100 µL MOG 35-55 peptide (Protein and Nucleic Acid Facility, Stanford University School of Medicine) in complete Freund’s adjuvant supplemented with
4mgmL-1 heat-inactivated Mycobacterium tuberculosis H36a (Sigma-Aldrich). A total of 50 µL emulsion was deposited on either side of the tail base. Pertussis toxin (300 ng per 200 µL; 180, List Biological Laboratories) was intraperitoneally injected on days 0 and 2 after MOG immunization. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Chemicals and reagents

Toxins used for Drosophila feeding assays (Table 1) were purchased from List Biological Laboratories (Campbell, California).
Toxins (product numbers) used for screening in Drosophila included:
Bordetella pertussis toxin (180) and adenylate cyclase toxin (188L), Clostridium difficile toxins A (152C) and B (155B), Clostridium perfringens ε-toxin (126A), Clostridium septicum α-toxin (116L), Corynebacterium
diphtheriae toxin (150), Escherichia coli O111:B4 lipopolysaccharide (201), Pasteurella multocida toxin (156), Salmonella typhimurium lipopolysaccharide (225), Salmonella minnesota R595 lipopolysaccharide (304) and monophosphoryl lipid A (401), and Vibrio cholerae cholera toxin
(100B).

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #188L – Adenylate Cyclase Toxin, Recombinant from B. pertussis
• Product #152C – Toxin A from Clostridium difficile
• Product #155 – Toxin B from Clostridium difficile
• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae
• Product #201 – LPS from Escherichia coli O111:B4
• Product #156 – Pasteurella multocida Toxin
• Product #225 – LPS from Salmonella typhimurium
• Product #304 – LPS from Salmonella minnesota R595 (Re)
• Product #401 – LIPID A monophosphoryl from Salmonella minnesota R595
• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

EAE Induction

Female C57BL/6J mice at 6-8 weeks old were purchased from Experimental Animal Center of Air Force Military Medical University. EAE was induced with 200 ug MOG35-55 peptide (NJPeptide, China) emulsified in incomplete Freund’s adjuvant (Sigma – Aldrich, USA) added with 5 mg/mL Mycobacterium Tuberculosis H37RA (Difco, USA). Each mouse was immunized with the emulsion subcutaneously into four sites at the back followed by intraperitoneal injection of 200 ng pertussis toxin (List Biological Laboratory). The pertussis toxin was given again at day 2 post-immunization (p.i.). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Active EAE was induced in 9-12 week-old male and female mice by subcutaneous injection of 150µg of mouse myelin oligodendrocyte glycoprotein peptide (residues 35-55; MOG35-55) (Genemed Synthesis, Inc., San Francisco, USA) emulsified in Complete Freund’s Adjuvant (CFA) (Thermo Fisher Scientific; DF0639606) containing 5 mg/mL heat-inactivated Mycobacterium tuberculosis (Thermo Fisher Scientific; DF3114338). 250 ng of Bordetella pertussis toxin (List Biological Laboratories, Inc. #181) was administered intraperitoneally on the day of immunization and 48 hours later …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Whole toxin B (List Biologicals, Campbell, CA) was used to coat the wells of a U-bottom high-binding 96-well ELISA plates (Greiner Bio-One, Frickenhausen, Germany) …

• Product #155 – Toxin B from Clostridium difficile

Cholera Toxin 1mg 100B Hersteller: List Biological Laboratories; …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… After a 24-hour period, the culture medium was removed and replaced with fresh medium containing the desired concentrations of LPS … , MPLA (Re 595 Minnesota, list biological laboratories #401), …

• Product #401 – LIPID A monophosphoryl from Salmonella minnesota R595

… Mice were also administered 200 ng of pertussis toxin (List Labs, Campbell, CA, USA) on day 0 and day 2. Animals were weighed and scored daily for disease signs using …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Purified LPS was purchased from List Biological Lab. …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://www.listlabs.com/product-information/lipopolysaccharides/