Citations

… Pertussis toxin (PTX) was purchased from List Biological Laboratories (CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction

EAE was induced by immunization of 8–12 week-old mice with 200 μg of MOG35-55 peptide (GenScript) emulsified in complete Freund’s Adjuvant (400 μg desiccated Mycobacterium tuberculosis H37 Ra mixed with incomplete Freund’s adjuvant (BD Biosciences)). The emulsion was injected subcutaneously in a total volume of 100 μL in the hind flank under isoflurane anaesthesia. Intraperitoneal (i.p.) injections of 200 ng pertussis toxin (List Biological Laboratories) were administered on the day of immunization and 48 hours later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.2 Surgeries and tracer infusions

… Combinations of two retrograde tracer injections at different cortical locations were used. The tracers were fast blue (FB, Sigma-Aldrich, Gillingham, UK; 3% solution in PBS) and non-conjugated cholera-toxin b (CTB, List Biological Laboratories Inc, CA; 1% solution in .05-M tris). These two tracers exhibit different patterns when filling neuronal cell bodies (Köbbert et al., 2000). All FB injections were made mechanically and CTB injections were made either mechanically (22 cases) or iontophoretically (2 cases). All mechanical injections used a 1.0-μl Hamilton Syringe (Hamilton, Bonaduz, Switzerland). A dedicated syringe was used for FB and another for CTB. The tracers were infused at a flow rate of 20 ηl/min for 3–5 min, and the needle was left in situ for further 5 min before retraction. Iontophoretic injections were infused by a 6 s on/off pulse with 2-, 6- and 7-μA current for approximately 5 min each setting (total 15 min). The volume of the FB injections was 25–150 ηl (M = 73.75 ηl) and for CTB injections was 60–180 ηl (M = 78.33 ηl). …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Two intraperitoneal injections of pertussis toxin, Bordetella pertussis (300ng) (List Biological Labs), were also administered to all mice on the day of induction and 48h thereafter …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin (PTx) was obtained from List Biologicals (Campbell, CA, USA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice

EAE induction was carried out using our standard protocol (Raveney et al, 2015). Briefly, mice were injected subcutaneously with 100 μg MOG35–55 peptide (synthesized by Toray Research Center, Chūō-ku, Tokyo, Japan) and 1 mg heat-killed mycobacterium tuberculosis H37RA emulsified in complete Freund’s adjuvant (Difco, KS, USA). One hundred nanogram Pertussis toxin (List Biological Laboratories, CA, USA) was injected intraperitoneally (i.p.) on days 0 and 2 after immunization. All peptides were synthesized by Toray Research Center (Chūō-ku, Tokyo, Japan).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE model

Female Lewis rats at 6-8 weeks of age were purchased from Beijing Vital River Laboratory Animal
Technology Co., Ltd (Beijing, China). The antigen emulsion was prepared from an emulsion of guinea pig
spinal cord white matter homogenates in phosphate-buffered saline (PBS, 50% w/v) mixed with an equal
volume of complete Freund’s adjuvant (Sigma-Aldrich) supplemented with 4 mg/mL Mycobacterium
tuberculosis. Each rat was immunized by subcutaneous injection of antigen emulsion at a dose of 0.75
ml/kg into its footpad, followed by an auxiliary injection of 8 μg/kg diluted in 0.5 mL pertussis toxin (List
Biological Laboratories) in the dorsum of the foot. The second immunization was administered 7 days
after the first immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE

Pertussis injection solution (per mouse)

Reagent Stock concentration Final concentration Amount
Pertussis 100 μg/mL 2 μg/mL 2 μL

Pertussis toxin List Biological Laboratories Cat# 181

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… 0.1mL PBS containing 200ng pertussis toxin (List Biological Laboratories) was administered intravenously at day 0 and day 2 post immunization per mice. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… complete Freund’s adjuvant containing 4 mg/mL H37Ra M. tuberculosis was used for C57BL/6J and CD38KO mice, followed by the administration of pertussis toxin (200 ng per mouse; List Biological Laboratories, Inc.) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)