Citations

… Pertussis toxin (PTX) (300 ng; List Biological Laboratories) was given intraperitoneally on the day of immunization and 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Endotoxin derived from Escherichia coli O55:B5, Salmonella minnesota R595 Re and Salmonella typhimurium were obtained from the List Biological Laboratories, Inc. (CA, USA)…

• Product #203A – LPS from Escherichia coli O55:B5
• Product #304 – LPS from Salmonella minnesota R595 (Re)
• Product #225 – LPS from Salmonella typhimurium

Experimental autoimmune encephalomyelitis (EAE) induction and scoring

On the day of immunization (Day 0), mice were injected subcutaneously over each shoulder with 100 μL of an emulsion containing 0.5 mg/mL MOG35-55 peptide (Bio-Synthesis, 12-668-01) and 1.25 mg/mL heat-killed Mycobacterium tuberculosis (Becton, Dickinson, & Company, 231-141) in Complete Freund’s Adjuvant (Sigma Aldrich, F5881). Mice were intraperitoneally injected with 200 ng of Pertussis toxin (List Biological Laboratories, 180) on days 0 and 2 post-immunization. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… cholera toxin (100 ng/mL; List Biological Laboratories). SUM159 cells were cultured in Ham’s F-12 medium with 5% fetal bovine serum (FBS), 5 µg/mL insulin, and 1 µg/mL hydrocortisone. Hs578T cells were cultured in DMEM supplemented with 10% FBS and 40 µg/mL insulin. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… For Tregs depletion, Foxp3-DTR mice were injected intraperitoneally with 1 µg DT (List Biological Laboratories, Inc, USA) 24 h before BCAS, then 0.5 µg every three days …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Pertussis toxin (PTX) (180243A1) was purchased from List Biological Laboratories (Campbell, USA) …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Primary antibody Goat anti-CTB List Biological 1:1000 …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Pertussis toxin List Biological Laboratories Cat#: 181

EAE induction
Various groups of mice at 8-12 weeks were subcutaneously (s.c.) injected over four sites in the flank with 100 μL of emulsified IFA supplemented with 200 μg of MOG33–55 and 500 μg of heat-killed M.tb as previously described (Ouyang et al., 2019; Xiao et al., 2015). Three hundred ng pertussis toxin (List) per mouse in PBS was injected intraperitoneally (i.p.) at the time of immunization and 48 h later.

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

Immunoblotting and pull-down assays

Cell lysates and cell culture supernatants were separated by 10% or 15% SDS-polyacrylamide gel electrophoresis under reducing conditions (non-reducing when using the antibody against C5), transferred to a nitrocellulose membrane and blocked with 5% non-fat dry milk in 0.1% Tween 20/TBS solution. The membranes were probed with primary goat antibodies against PA (1:5000, List Biological Laboratories), …

Mouse Model of EAE

Female mice at 6 to 8 wk of age were immunized subcutaneously with 50 to 100 μg of MOG35-55 peptide (MEVGWYRS PFSRVVHLYRNGK; GL Biochem) emulsified in complete Freund’s adjuvant (Sigma) and supplemented with 400 to 800 μg of Mycobacterium tuberculosis H37Ra (Difco). The mice were intraperitoneally injected with pertussis toxin (200 ng/mouse; List Biological Labs) in 100-μL phosphate-buffered saline (PBS) on the same day and 2 d later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)