Bacterial Toxin Research Citations

Mouse techniques

… For diphtheria toxin receptor (DTR) ablation studies, WT and indicated DTR mice received injection twice intratracheally (i.t.) with 100 ng diphtheria toxin (DT) in 20 μl of sterile PBS or i.p. with 200 ng DT in 100 μl of sterile PBS (150, List Biological Lab) on 1 d before and after metastatic inoculation. Cell of interest depletion was confirmed by flow cytometry. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

… Pertussis toxin (180) was from List Biological Laboratories. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

Mice and EAE induction

We purchased C57BL/6, (C57BL/6xSJL) F1, 2D2-TCR-Tg, and DO11.10-TCR-Tg mice from the Jackson Laboratory. Mice were maintained in a specific pathogen–free facility and used under protocols approved by the National Institute of Allergy and Infectious Diseases (NIAID) Animal Care and Use Committee (protocol LISB-7E). EAE was induced by immunization of female C57BL/6 mice (10 to 12 weeks old) with 200 μl of emulsion containing 100 μg of MOGp35–55 and 400 μg of Mycobacterium tuberculosis extract H37 Ra (Difco) in Freund’s complete adjuvant (FCA) as previously described (73). At 4 and 24 hours after immunization, mice received 200 ng of pertussis toxin (List Biological Laboratories) intraperitoneally. To induce RR-EAE in (C57BL/6xSJL) F1 mice, we immunized the female F1 mice with 200 μl of emulsion containing 200 μg of MOGp35–55 and 400 μg of M. tuberculosis extract H37 Ra (Difco) in FCA. At 4 and 24 hours after immunization, mice received 75 ng of pertussis toxin intraperitoneally. EAE was scored by the methods previously described (74, 75). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

5.6. The establishment of EAE model in mice

The EAE model was induced and evaluated as previously described48. Briefly, 2 mg/mL of MOG35-55 (Glbiochem, Shanghai, China) was emulsified in complete Freund’s adjuvant (CFA) that contained 5 mg/mL of non-viable Mycobacterium tuberculosis H37RA (BD Biosciences) and Freund’s incomplete adjuvant (MOG35-55 was emulsified with CFA in a 1:1 volume ratio). Subsequently, 200 μg emulsified MOG35-55 was injected subcutaneously into each mouse on the posterior aspect of both ears and thigh bones on Day 0. Later, 300 ng PTX (List labs, CA, USA) in phosphate-buffered saline (PBS) was administered to each mouse via intravenous injection on Day 0 and Day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin(PTX,cat#179A) was purchased from List Biological Labs(California, USA). …

2.5 EAE model establishment and treatment

A total of 50 mice were randomly divided into 5 groups (10 mice/group) as follows:
control group (CON), EAE model group (MOD), SJP low-dose group (SJP-L), SJP high-dose group (SJP-H), and positive control Prednisone acetate tablets group (PAT). The experimental autoimmune encephalomyelitis model was established as described previously(Shaw et al., 2011; Stromnes and Goverman, 2006). All mice were injected subcutaneously with an antigen-mixed emulsion (200 μg of MOG35-55 emulsified in a 1:1 ratio with CFA containing 4 mg/mL inactivated MTB) on day 0, followed by intraperitoneal injection of 500 ng PTX on days 0 and 2 to induce the EAE. …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… In brief, mouse peritoneal cells were primed with the indicated concentration of ultra-pure lipopolysaccharide (LPS; List Biological Lab) for 3 h and then stimulated with indicated concentration of particles …

Author did not specify which List Labs LPS product was utilized in their research.
List Labs provides the following LPS products: https://listlabs.com/product-information/lipopolysaccharides/

… Cholera Toxin B Subunit (CTB) was purchased from List Biological Laboratories, Inc (Campbell, CA, USA) …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… 400 ng Pertussis toxin (List Biological Laboratories Inc, USA) was administered at 0 h and after 48 h via intraperitoneal (IP) injection. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.3 Induction of antibody-augmented MOG35-55 EAE

Active EAE was induced in 8-10 weeks old mice. MOG peptide 35-55 (MOG35-55, 100 µg; Institute of Medical Immunology, Charité Berlin, Germany) emulsified in complete Freund’s adjuvant (CFA) was injected subcutaneously (s.c.) under anesthesia (50 mg/kg Ketamine; Ketanest®, Zurich, Switzerland and 1 mg/kg medetomidine; Dormitor®, Provet, Lyssach, Switzerland). After 30 minutes, mice were awakened by a s.c. antagonist injection (Atipamezol 1.25 mg/kg; Antisedan®, Provet), hydrated with 300 µl sodium chloride 0.9% solution and observed until fully recovered. Zero and two days post immunization (dpi), 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA, USA) was injected intraperitoneally (i.p.). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Pertussis toxin List Labs Cat#181 …

EAE induction
8-week-old, female C57BL/6 mice were s.c. injected with MOG35–55 peptides (300 mg per mouse) emulsified in complete Freund’s adjuvant (CFA) (200 μL per mouse) containing dead Mycobacterium tuberculosis (1 mg per mouse) on day 0. On days 0 and 2, pertussis toxin (400 ng per mouse) was intraperitoneally injected into mice. …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)