Citations

… For double immunostaining with mCherry and CTB, sections were incubated overnight with a mixture of rabbit anti-DsRed antibody (1:1000 dilution; Clontech) and goat anti-CTB antibody (1:10,000 dilution; List Biological Laboratories, Campbell, CA, USA; Cat. # 703; RRID:AB_10013220) in PBS-XD …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Pertussis toxin (PTX) was purchased from List Biological Labs (California, USA)…

EAE model induction and animal behavior test

Firstly, the mice were randomly divided into the normal control (NC) group, EAE group, low-dose emodin group (Emodin-L), and high-dose emodin group (Emodin-H) (n = 9 in each group). The mice of EAE and emodin groups received subcutaneous injections of 200 µg MOG 35–55 emulsified 1:1 in CFA containing 4 mg/mL heat-killed MTB on day 0, followed by 500 ng PTX intraperitoneally on days 0 and 2 to induce the EAE [19,20]. Next, NC and EAE mice were treated with sodium carboxymethyl cellulose. While the mice of Emodin-L and Emodin-H groups were treated with 30 mg/kg/d and 60 mg/kg/d emodin, respectively. The treatment by oral gavage started at day 0 days post immunization (dpi). Then, test animal behavior and pathological changes by HE and IHC of BDNF and MBP. Second, choose the best dose of emodin group to have further investigation. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Immunized mice received pertussis toxin (List Biological Laboratories) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.2. MC Treatment

For G proteins and Ca++ channel inhibition, cells were pretreated for 16 h with PTX at 200 ng/mL (Gαi inhibitor, List Biological Labs, Campbell, CA, USA), …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Retrograde tract-tracing experiments in rats

The retrograde tracer, cholera toxin ß subunit (CTB; List Biological Laboratories, Campbell, CA), was injected iontophoretically into the hypothalamic tuberomammillary nuclei (TMN), targeting E1-E2 subdivisions at stereotaxic coordinates from the bregma: AP= -3.84 mm, DV= -9.6 mm, L= -1.5 mm (n= 19 rats), …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Experimental autoimmune encephalitis (EAE)

Mice were given 1 × 108 EID50 HKx31 i.p., and then rapamycin or PBS, daily as described above. Mice received rapamycin or PBS for 14 days, and then were rested for two weeks without additional injections to be sure the rapamycin was not present at the time of EAE induction. EAE was induced by injecting mice subcutaneously (s.c.) with an emulsion of 100 μg of mouse native myelin basic protein (Creative Biomart) with 400 μg of CFA (Chondrex). Mice also received 200 ng of pertussis toxin (List Biologicals) i.p. at the same time as induction, and two days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice and Immunizations.

Eight-week-old female WT B6 mice (Charles River) were kept in specific pathogen-free conditions at the animal facility of the Franz-Penzoldt-Zentrum, Erlangen, Germany (approval by the Regierung von Unterfranken, RUF-55.2.2-2532-2-575-5). All animal experiments complied with the German Law on the Protection of Animals, the “Principles of laboratory animal care” (44) and the ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines (45). For immunization, incomplete Freund’s adjuvant (IFA) was prepared by mixing paraffin oil and mannide monooleate (both Sigma Aldrich) in a 9:1 ratio. CFA was subsequently obtained by adding 5 mg/mL Mycobacterium tuberculosis H37 Ra (Difco) to IFA. A total of six cohorts of B6 mice (n = 6 to 10 per cohort) were immunized subcutaneously in both sides of the flank with casein solution from bovine milk (Sigma Aldrich) emulsified (1:1) in CFA, resulting in a total injection of 200 μg of antigen per mouse. Additionally, 200 ng PTx (List Biological Laboratories) was injected intraperitoneally on the day of immunization and 48 h later. One cohort each of casein-immunized mice was killed at day 13 or day 20, three cohorts were killed at day 40, and the last cohort was killed at day 60. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction and treatment of experimental autoimmune uveitis

For EAU induction, mice were subcutaneously immunized with an emulsion containing equal volumes of incomplete Freund’s adjuvant (IFA) (Sigma-Aldrich) with 5 mg/mL desiccated Mycobacterium tuberculosis (Sigma-Aldrich) and 250 μg IRBP651–670 (Hanhong group, Shanghai, China) in PBS, distributed at 4 spots on the tail base and flank. Mice were intraperitoneally administered 500 ng pertussis toxin (PTX) (List Biological Laboratories, California, USA) on the day of immunization and 24 h post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

PTX List Biological Labs, Inc #181

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

… Pertussis toxin (400 ng, Cedarlane List Biological labs) was injected intraperitoneally 48 hours after EAE induction. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)