Bacterial Toxin Research Citations

Induction and assessment of EAE

8-week-old male C57BL/6 mice were injected subcutaneously in the dorsal flanks with 3 mg/ml MOG35-55 peptide (300 μg per mouse) in CFA containing 5 mg/ml heat-killed Mycobacterium tuberculosis (500 μg per mouse) on day 0. Mice were injected with Pertussis toxin (150 ng per mouse, list labs) on days 0 and day 2 through the retroorbital vein. In Tivantinib prevention trails, Tivantinib was intraperitoneally injected into mice (10 mg/kg) beginning at day 0 to day 22 every two days. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Determination of intracellular cytokine induction following SIV-Gag peptide stimulation

… Staphylococcal enterotoxin A & B (SEB/A-List Biologicals stimulation at 250 ng ml−1 served as a positive control. Peptide diluent (1% dimethyl sulfoxide) served as the negative control. …

… Protective antigen List Biological Labs #171E …

• Product #171E – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Enzyme-linked immunosorbent assay

Nunc-immuno microtitre plate wells with Maxisorp surface (Thermo Fisher) were coated in triplicates with 30ng/well of full-length TcdA (List Biological Laboratories, Inc., Campbell, CA) in 0.05M carbonate–bicarbonate buffer pH 9.6, and incubated overnight at 4°C. Wells were blocked with 1% (w/v) bovine serum albumin in PBS for 1h at room temperature, washed with PBS/0.05% (v/v) Tween 20 and then incubated for 1h with decreasing concentrations of His6-tagged VHH constructs in PBS containing 0.2% bovine serum albumin and 0.05% Tween 20 (PBS-TB). …

• Product #152C – Toxin A from Clostridium difficile

… Unless otherwise specified, mice were sensitized by a single intraperitoneal injection of 250 µg ovalbumin (Sigma; A5478) and 5 µg cholera toxin (List Labs; 100B) in PBS …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… For oral sensitization experiments, mice were given oral gavages on days 0 and 7 with 5 mg of grade III OVA with 0.5 mg/kg of cholera toxin (List Biologicals 100B, lot nos. 10165A1 and 10165A2) in a final volume of 0.25 mL 0.2 M sodium bicarbonate buffer. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… All mice were anesthetized with isoflurane (2%) in 100% oxygen as described previously, while injections of CTb 1% (low salt, 1%, List Biological Laboratories, Campbell, CA) in distilled water, were made using a glass micropipette (tip diameter 15-20 µm) and Nanoinject II, to retrogradely …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… CTb was detected using a polyclonal goat anti-CTb (List Laboratories; …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Pertussis toxin (200 ng, List Biological Laboratories) was injected intraperitoneally on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 100 ng/ml cholera toxin (List Biological Labs), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae