Bacterial Toxin Research Citations

… Mice were orally immunized with 10 µg of CT (List Biological Laboratories, Campbell, CA) and, when indicated, admixed with OVA at 10 mg per dose and dissolved in 0.5 ml of phosphate-buffered saline (PBS) containing 3% (w/v) NaHCO3 …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Where indicated, mice received 10 µg of cholera toxin (List Labs) by oral gavage. In some cases, mice were immunized intraperitoneally with 10 µg of peptides plus 20 µg …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Chronic EAE.

Six- to eighteen-week-old female C57BL/6 or DEREG Foxp3-DTR (diphtheria toxin [DT] receptor) mice were immunized with
50 mg MOG35 55 (AnaSpec) in CFA consisting of IFA (Difco Laboratories) and 5 mg/ml M. tuberculosis H37RA (Difco Laboratories). A total of 200 ng pertussis toxin (List Biological) was injected i.p. on days 0 and 2 p.i. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… emulsified in complete Freund’s adjuvant (CFA) (Sigma-Aldrich, St. Louis, MO, USA) mixed with 5.0 mg/mL Mycobacterium tuberculosis strain (MTB), and then intraperitonealy injected with 500 ng of pertussis toxin (List Biological, Campbell, CA, USA) as described previously …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… based on their ultrastructural level, we injected CTB (List Biological Laboratories, CO, USA) into the TA muscle. Retrograde labeling was observed at the light microscope …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

2.2 Active experimental autoimmune encephalomyelitis induction and control immunization

aEAE was induced in both male and female mice by injection of myelin oligodendrocyte glycoprotein peptide 35–55 (MOG35-55 peptide, 200 μg per animal, Genscript) and complete Freund’s adjuvant (Incomplete Freund’s Adjuvant, Santa Cruz Biotechnology; supplemented with Mycobacterium tubercolosis, Difco). An emulsion of MOG35-55 and CFA was injected subcutaneously in mouse flanks and at the tail base at day 0; in addition, 300 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) was injected intraperitoneally at day 0 and day 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

TLR agonists were: … Monophosphoryl Lipid A (MPLA) (List Biologicals, Campbell, California), …

• Product #401 – LIPID A monophosphoryl from Salmonella minnesota R595

Pertussis Toxin List Biologic Cat# 180

Induction and evaluation of EAE

To induce EAE, eight- to 12-week-old mice were injected subcutaneously with an emulsion contains 200 μg MOG35-55 in complete Freund’s adjuvant (CFA) containing 500 μg of nonviable, desiccated Mycobacterium tuberculosis at a concentration of 10 mg/mL on upper and lower back. Mice were i.p. injected with 200 ng pertussis toxin (PT) 2 hours later, and then repeated 2 days after immunization. For adoptive transfer of EAE with myelin-reactive T cells, splenocytes were isolated from MOG TCR transgenic (2D2) or wild type EAE mice. After removal of red blood cells by ammonium-chloride-potassium (ACK) lysis buffer, splenocytes were collected and suspended in RPMI-1640 medium containing 10% FBS. T cells were stimulated with 10 μg/ml MOG35−55 peptide for 48 h, and then expanded in 5 ng/ml IL-2 and IL-7 for 5 days. …

• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer

… Pertussis Toxin from List Biological Laboratories was dissolved in water …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Table 1Summary of primary antibodies and dilutions for the immunolabeling. IF: immunofluorescence. IHC: immunohistochemistry (peroxidase).

Antibody Host Antigen Manufacturer RRID Dilution
CTB Goat/Polyclonal Cholera Toxin B List Biological Lab., CA, USA AB_10013220 1:5000 (IF)

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)