IL-10 knockout (KO) mice can be protected against experimental autoimmune encephalomyelitis (EAE)
with low-dose estrogen (E2) treatment similar to wild type (WT) mice, indicating that IL-10 is not required
for E2-induced EAE protection. Our previous study demonstrated that E2 treatment induced an increase in
programmed death ligands 1 (PD-L1) and 2 (PD-L2) on monocytes and macrophages in the periphery
and within the CNS. In this study, we selectively inhibited the function of PD-L1 and PD-L2 to evaluate
their critical role in maintaining E2-induced protection against EAE in IL-10-KO mice.