Citations

Active and passive EAE induction:

For active EAE induction mice were immunized s.c. at the lateral pectoral regions with 2 × 100 µg murine MOG₃₅₅₅ peptide (MEVGWYRSPFSRVVHLYRNGK; GenScript) emulsified in CFA (BD DifcoTM) containing 4mg/ml Mycobacterium tuberculosis H37 Ra (BD DifcoTM). Two i.p. injections of 200ng pertussis toxin (List Biological Laboratories) in PBS were given 0 and 2 days post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 1 µg cholera toxin (CT) (List Biological Laboratories…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials:

… Pertussis toxin (PTx) was from List Biological Laboratories (Campbell, CA, USA). …

Degranulation Assay:

The degranulation was measured by -hexosaminidase release as described previously [51]. Briefly, transfected RBL-2H3 cells (5 10⁴ cells per well) were seeded into a 96-well, white, clear-bottom cell culture plate and incubated overnight in a 37 C incubator with 5% CO₂. To determine the inhibitory effects of PTx and YM-254890 on MC degranulation, cells were pretreated with PTx (100 ng/mL, 16 h) and/or YM-254890 (10 M, 5 min) prior to stimulation with SP. Cells were then washed twice and suspended in a total volume of 50 L HEPES buffer containing 0.1% bovine serum albumin (BSA). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Half milliliter containing 400,000 equine monocytes (ratio 1:4; n=3) were added to each insert of a transwell plate with pore size of inserts 0.4 m (Corning, NY), stimulated with 50 ng/ml of E.coli 0111:B4 LPS (List Biologicals, Inc) and incubated at 37C, 5% CO2. …

• Product #201 – LPS from Escherichia coli O111:B4

… As in our previous studies (Llewellyn-Smith and Mueller, 2013; Mischel et al., 2014), all rats (11 sedentary, 10 active) received spinal cord injections of cholera toxin B subunit (CTB; List Biologicals, Campbell, CA) seven days prior to sacrifice. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… injection into the hind flank. Pertussis toxin (500 ng; List Biological Laboratories, Inc., Campbell, CA, USA) was injected intraperitoneally at days 0 and 2 post-immunization (PI). Following immunization, …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

PTX and TCDD Administration:

… Groups of 3–5 mice were first injected IP with vehicle (CO), or TCDD (25 μg/kg body weight) then injected IP with 400 ng/mouse PTX (List Biological Laboratories, Campbell, CA, United States). Twenty-four hour later, the mice were rechallenged with the same dose of PTX intraperitoneally, as described (Elliott et al., 2018).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Briefly, the mice were subcutaneously injected with 200 µg MOG 35-55 in complete Freund’s adjuvant (5 mg/ml, Sigma-Aldrich). Pertussis Toxin (200 ng, List Biological Laboratories) was injected intravenously twice on 0 and 2 days postimmunization (dpi.) …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Instead of BDA cholera toxin B subunit (0.5% in 0.1 N PB; CTB, low-salt cholera toxin B subunit, List Biological Laboratories, Campbell, CA) was injected iontophoretically by passing positive current (4 A, 1 Hz, 10 % duty cycle) for 10 minutes into the GL of the medial …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Antibodies and chemicals:

… pertussis toxin (179B) was from List Labs. …

• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)