Citations

Pertussis toxin (200 ng, List Biological Laboratories) in PBS was administered i.v. on days 0 and 2. Mice were examined daily and scored for disease severity using the standard scale: 0, no clinical signs; 1, limp tail; 2, paraparesis (weakness, incomplete paralysis of one or two hind limbs); 3, paraplegia (complete paralysis of two hind limbs); 4, paraplegia with forelimb weakness or paralysis; 5, moribund or death. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… 1:1,000 for antiEF and antiLF sera, and antiPA antibody (List Labs, Campbell, California). …

First step c-Fos/CTb

Of the 28 animals used, 8 showed a suboptimal localization of the CTb and were excluded from this part of the study, therefore number of animal for each group changed to: Torpor n=4, Fasting n=4, Cold Exposure n=4, Control n=8. Following the blocking solution, all slices were incubated at room temperature overnight with the following primary antibodies: i) goat Anti-CTb (List Biological Laboratories); …

Second step neuronal typization

… 5.  Slices were then rinsed twice in PBS with 0,3% (v/v) Triton X-100 and incubated overnight at room temperature with: i) goat Anti-CTb (List Biological Laboratories; 1:10000); ii) rabbit Anti-c-Fos (Calbiochem; 1:10000).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

E2 pellet implantation and induction of EAE

… Additionally, mice were administered 75 ng of pertussis toxin (Ptx, List Biologicals, Campbell, CA, USA) via an intraperitoneal (i.p.) injection on the day of immunization and 200 ng i.p. 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… The retrograde tracer, cholera toxin subunit (CTB; List Biological Labs, Campbell, CA, USA CTB serum (1:10000, List Biological Labs, Campbell, CA, USA) for 2 days. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Vaccine preparation and immunization of mice

… For I.N. immunizations, WCA (100 μg) was diluted in saline and combined with CT (List Biological Laboratories, Campbell, CA) (0.1 μg/10 μl) and administered to the nares of mice twice at weekly intervals. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Study design and animal model:

… EAM was induced by intraperitoneal injection of pertussis toxin (400ng, List Biological Laboratories) followed by subcutaneous injection of MyHC peptide …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunisation, H. pylori challenge and bacterial quantification in the stomach:

Groups of mice (n=5) were immunised orally four times in two rounds on days 0 and 1; 14 and 15. One hour prior to immunisation, food was withdrawn. Mice were orally gavaged with 200l of 0.3M sodium bicarbonate buffer. After 20min, mice were gavaged with 200l of sterile Phosphate-buffered saline (PBS) containing 110⁹ formalin-killed H. pylori Hel 305 with or without -GalCer (10g) (Avanti Lipids) or CT (10g) (LIST Biologicals), or with PBS alone. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Perfusion and tissue processing

… For CT-B processing, sections were first blocked for endogenous peroxidases (Bloxall), washed (0.1M TBS-TX), and then treated with 10% horse serum (2h, at room temperature). Sections were then incubated in anti-CT-B made in goat (List Biological #703; 1:4000 dilution in TBS-TX) and 3% horse serum for 2-3 days at 4°C. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… Pertussis toxin (List Biological Laboratories Inc., Campbell, CA) was injected on days 0 and 2 (100 ng per mouse, intraperitoneally). …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)