Bacterial Toxin Research Citations

Anthrax protective antigen and hepatitis B surface antigen ELISAs:

Ninety-six-well microtiter plates (Corning, Lowell, MA) were coated with 1 g/well of recombinant PA (List Biological Laboratories, Campbell, CA) …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Retrograde tracer injection and histology:

Rats were anesthetized with a subcutaneous injection of a solution containing ketamine (5 mg/100 g), xylazine (1 mg/100 g), and acepromazine (0.2 mg/100 g) and received a stereotaxic injection of CTb (1%; List Biological Laboratories, Campbell, CA, USA) into the PMV (n=32 males and n=30 females). The CTb was injected iontophoretically from a glass micropipette (1020 m of tip internal diameter) by applying a +5 A current pulsed at 7-s intervals over 1012 min. …

Immunohistochemistry:

One series from each animal was submitted to a standard immunoperoxidase reaction. Sections were pretreated with hydrogen peroxide and blocked in 2% normal donkey serum (Jackson Laboratories, West Grove, PA, USA) and 0.3% Triton X-100 (Sigma). The sections were then incubated with antisera against CTb (raised in goat, 1:50,000; List Biological Laboratories, Campbell, CA, USA) overnight at room temperature. The CTb antisera have been validated before …

Author did not specify which CTB was utilized.  List Labs provides Product #103B (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae)  and Product #104 (Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt).

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

… E. coli lipopolysaccharide (LIST Biologicals). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE induction and evaluation

EAE was actively induced by subcutaneous injection of MOG35-55 peptide (MEVGWYRSPFSRVVHLYRNGK) as previously described [17], [22]. Briefly, 8–10 week-old female wild-type mice were anesthetized with intra-peritoneal injection of atropine (0.6 mg/kg body weight) and 2.5% avertin (0.02 ml/g body weight). 300 µg MOG was emulsified in complete Freund’s adjuvant (CFA, DIFCO) supplemented with 500 µg of heat-inactivated Mycobacterium tuberculosis (DIFCO) and injected into the mouse hind-flank on day 0. On day 7, mice were boosted with injection of 300 µg of MOG into the other flank. 500 ng pertussis toxin (List Biologicals) was injected intra-peritoneally on day 0 and 2.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials:

…and pertussis-toxin from List Biological Laboratories (Campbell, CA, USA). …

Pertussis toxin inhibits mH₄R- but not mH₁R-mediated effects:

Experiments with pertussis toxin (PTX), which selectively inhibits G_i-protein activation by GPCRs [41], [42], were performed. HEK293 mH₁R and HEK293 mH₄R cells were incubated with or without PTX for 18 h and then the [Ca²⁺]_i and the forskolin-induced cAMP concentrations were evaluated after stimulation with HA. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Protocols:

All methods have been described recently. [8] In brief, studies were performed in male C57Bl/6 mice weighing 2530 g (Charles River Laboratories). Mice were injected i.p. with LPS (Escherichia coli 055, LPS no. B5, List Biological Laboratories, Campbell, CA) at a dose of 10 mg/kg (volume diluted with saline in 0.5 ml) once a week for 14 weeks. Mice were injected with 0.5 ml of saline as a control. Mice were monitored daily…

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

EAE Induction and in Vivo Treatments:

EAE was induced as described (16) with minor modifications. Eight to twelve-week-old mice were actively immunized with 100 µl of 1 mg/ml MOG(3555) peptide… At immunization and 48 h later, all mice received 500 ng of pertussis toxin (List Biological Laboratories) intraperitoneally.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Autoimmune Encephalomyelitis:

The mice received three daily intraperitoneal (i.p.) administrations of 500 ng of pertussis toxin (PT) (List Biological Laboratories) starting on day 1. The disease severity was scored as previously described (Miyake et al., 2013). For the in vitro restimulation analysis, cells were collected from the axillary, inguinal, and lumbar (paraaortic) lymph nodes on day 23.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tetanus toxoid stimulation assay:

Total PBMCs from healthy donors were thawed in RPMI-1640 medium containing 10% heat-inactivated human serum (GemCell). Cells were washed twice with medium and suspended at a final concentration of 1.5 10⁶ cells/ml. Tetanus toxoid (List Biological Laboratories) was added at a concentration of 2.5g/ml. …

• Product #191 – Tetanus Toxoid from Clostridium tetani

EAE induction – C57BL/6J mice:

EAE was induced in ~5 week old C57BL/6J female mice (Jackson Laboratory). … Once completed, an intraperitoneal (i.p.) injection of pertussis toxin (PTX; 100 ng/100µl saline; List Biological Laboratories, Campbell, CA) was administered. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)