Bacterial Toxin Research Citations

Chemicals and reagents were purchased from: … phenol-extracted LPS (Escherichia coli O111:B4) free of lipoprotein contamination (List Biological Laboratories); …

Microparticle isolation and quantitation:

Washed platelets (109/ml) were pretreated with 30 M MG132, 40 M
bortezomib or buffer for 30 min before an overnight treatment with thrombin (0.2
U), ADP (100 M) or 0.1 g/ml LPS with addition of 0.1 g/ml each of human
recombinant CD14 and LPS binding protein (LBP). …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Use of RNAi in mouse macrophages to test candidate genes:

… Twenty-four hours after transfection, cells were incubated in the presence of 20 ng/ml LPS (List Biological Labs), and supernatants were collected for ELISA (R&D Systems). …

Overexpression studies in mouse macrophages:

… Twenty-four hours after transfection, cells were stimulated with LPS for 6 hr and then luciferase activity was monitored using the Dual luciferase assay kit (Promega) using SV40-rluc as a normalization control for transfection efficiency. …

Author has specified that the following LPS product was used:

• Product #201 – LPS from Escherichia coli O111:B4

EAE Induction and clinical evaluation:

For determining whether the bacterial expressed peptides similar to MOG could induced
active EAE in B6 mice (Figure 5F), mice were immunized subcutaneously with 100 µg
of MOG3949 or 11 amino acid versions of homologous foreign peptides emulsified in
CFA containing 4 mg/ml Mycobacterium tuberculosis. In addition, the mice received 200
ng of pertussis toxin (List Biological Laboratories) intravenously on the day of, and 2
days after, peptide immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… PTX was from List Biological Laboratories (Campbell, CA, USA). … 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… This was injected into both flanks and the base of the tail, in a total volume of 250 µl, immediately followed by intravenous injection of 300 ng pertussis toxin (List Biological, Campbell, CA) in 0.1 ml PBS. An identical pertussis toxin injection was repeated 3 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

On the day of immunization (day 0) and 48 h later (day 2), 200 ng pertussis toxin (List Biological laboratories Inc. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

Mice were subcutaneously immunized with 200 µg MOG_35-55 peptide emulsified in a equal volume of complete Freunds adjuvant…followed by two i.p. injections of 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA) 48 h apart. 

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and in vivo treatment with JTE-013:

EAE was induced in SJL/J mice via active immunization with proteolipid protein…In addition, mice were injected with 200 ng pertussis toxin (List Biologicals Laboratories Inc.) at the time of immunization and 2 days after.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials:

… Activated protective antigen [63 kDa (1 M=63g/ml); PA63] and recombinant lethal factor [90 kDa (1 M=90 g/ml); LF] were obtained from List Biological Laboratories (Campbell, CA). …

PA63 and LF were used in the following:

• Optimization of LTx complex detection assay
• Limit of detection and quantification
• Precision and Accuracy for PA83 and PA63 Detection
• Detection of PA63 and LTx in the presence of AIG

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #174 – Anthrax Protective Antigen, Activated (PA 63) from B. anthracis

… bilateral injections of two different tracers into the OB and prepiriform cortex, namely, biotinylated dextran amine (BDA; 10,000 molecular weight; Molecular Probes, Eugene, OR) on the right and cholera toxin B subunit (CTB; choleragenoid; List Biological Laboratories, Campbell …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt