Bacterial Toxin Research Citations

Cell Culture and Stimulation:

RAW264 and J774A.1cells were cultured in DMEM medium supplemented with 10% fetal bovine serum (FBS) at 5% CO2. Cells were stimulated with ultrapure smooth LPS (sLPS) from E. coli 0111:B4 (List Biological Laboratories) …

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

EAE:

Mice were injected subcutaneously with 200 µg MOG3555 (amino acid sequence, MEVGWYRSPFSRVVHLYRNGK; Anaspec, Fremont, CA) emulsified in complete Freunds adjuvant (Difco, Detroit, MI) supplemented with 4 mg/ml Mycobacterium tuberculosis H37RA (Difco, Detroit, MI). On d0 and d2 after immunization, the mice were injected intraperitoneally with 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA) in 100 µL PBS. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Immunizations and serum isolation:

Groups of eighteen (18) female C57Bl/6 (National Cancer Institute/Charles River Laboratories, Wilmington, MA) mice at 8-12 weeks of age were subcutaneously immunized with saline, 5 g recombinant anthrax protective antigen (rPA; List Biological Laboratories, Inc., Campbell, CA) alone or with 1.3 mg alum (Alhydrogel; Sigma, St. Louis, MO). On day 71 post-immunization, three mice from each group were given a boost of rPA (no adjuvant) at the same dose as the primary immunization …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Materials:

… CT was from List Biological Laboratories (Campbell, CA), …

CT intoxication assay:

CHO cells grown to 80% confluency in a 24-well plate were incubated in serum-free medium with 1, 10, or 100 ng/mL of CT for 2 hr at 37C. Toxin-challenged cells were either left untreated or were co-incubated with 50 M ribostamycin. The cAMP content of intoxicated and unintoxicated control cells was quantified with a commercial kit (Perkin-Elmer, Boston, MA) following the manufacturer’s instructions. Values obtained from unintoxicated cells were background subtracted from the results with intoxicated cells, and the experimental data were then expressed as percentages of the maximal cAMP response obtained from cells exposed to 100 ng/mL of CT in the absence of ribostamycin. Triplicate samples were used for each condition.

CT transport assay

CHO cells grown to 80% confluency in 6-well plates were pulse-labeled for 30 min at 4°C in serum-free medium containing 1 µg/mL of CT. Unbound toxin was removed by washing with PBS, after which the cells were returned to toxin- and serum-free medium for a 2 hr incubation at 37°C. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… Briefly, mice (n = 60) were orally sensitized with 1 mg of OVA [28] adjuvanated with 10 µg cholera toxin (CT; List Biologicals, CA, USA) for 4 wk (twice/week), and orally treated with various glycated OVA forms (1 mg) for 4 wk (three times/week). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Immunocytochemistry:

Mice were deeply anesthetized with a lethal dose of anesthetic…with primary antibodies overnight at 4oC. Primary antibodies and dilutions used were:…6) goat anti-cholera toxin, -subunit (1:4000; List Biological Labs, CA);…

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

EAE:

… Animals received 200 ng of pertussis toxin (List Biological Laboratories) ip on days 0 and 2. Clinical signs of EAE were assessed according to the following scores: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… Pertussis toxin (0.3 µg/200 µl, List Biological Laboratories, Campbell, USA) was administered intraperitoneally on the same day and again two days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…Pertussis toxin (300 ng in PBS; List Biological, USA) was injected intraperitoneally at the time of induction and a second dose was administered three days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Vasoactive amine sensitization and challenge:

Bphs was carried out as described… Bpss and Bpbs followed a similar protocol. Briefly, 200 ng of PTX (List Biological Laboratories, Campbell, CA) in phosphate-buffered saline was administered intravenously (i.v.) by tail vein injection in a volume of 0.1 ml on day 0. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)