Bacterial Toxin Research Citations

Experimental Autoimmune Encephalomyelitis (EAE):

Induction of EAE in C57BL/6 mice (N =18) was used as a model for multiple sclerosis (MS). EAE was induced by subcutaneous injections of 100 µg of myelin oligodendrocyte glycoprotein (MOG₃₅₅₅; MEVGWYRSPFSRVVHLYRNGK, AnaSpec Inc., Fremont, CA) in complete Freunds adjuvant (incomplete Freunds adjuvant containing 4 mg/ml of heat-inactivated Mycobacterium tuberculosis H37Ra; BD Biosciences, Mississauga, ON, Canada). An intravenous injection of 200 ng of pertussis toxin (PTX; List Biological Laboratories Inc., Campbell, CA) was also administered on days 0 and 2 of the immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

… Mice were also injected intraperitoneally with 400 ng of pertussis toxin (List Biological Laboratories, Inc., Campbell, CA, USA) on days 0 and 2 after MOG sensitization …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice, peptides and EAE induction:

…EAE was induced as described [17] by subcutaneous immunization with MOG₃₅₅₅ (100 g/mouse) in Complete Freund’s Adjuvant (CFA, Difco), containing 4 mg/ml of heat-killed Mycobacterium tuberculosis (Difco). All mice were injected i.v. with 200 ng/mouse of Bordetella Pertussis toxin (PTX, List Biological Lab) on day 0 and 2 post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and treatment:

…Chronic EAE was induced in the female C57Bl/6 mice by subcutaneous immunization with MOG₃₅₅₅ (Anaspec, San Diego, CA), which was emulsified in Complete Freund’s adjuvant with 8 mg/ml of Mycobacterium tuberculosis H35RA (Difco, Detroit, MI) in 11 ratio, and 100 µl (100 ng) of emulsified MOG₃₅₅₅ was injected sub-cutaneously at each side of the base of the tail respectively. Each mouse also received 100 µl (200 ng) of pertussis toxin (List Biological Laboratories, Campbell, CA) by intraperitoneal (i.p.) injection, and the injection of toxin was repeated 2 days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

 Construction of the 3D skin:

… 1 nM cholera toxin (List biological laboratories, Inc., Campbell, CA, USA); …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Cell Culture

… 100 ng/ml cholera toxin (List Biological Laboratories, Inc.), …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materialien und Durchfhrung:

Pertussis toxin List Biological Laboratories, Campbell, US …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental autoimmune encephalomyelitis (EAE) model:

C57Bl/6 non-transgenic and shCD5ETg mice were immunized with a single s.c. injection of 150 µg of MOG₃₅₅₅ peptide (BioSynthesis, USA) emulsified in CFA (Difco, supplemented with 400 µg/mL of Mycobacterium tuberculosis) on day 0, followed by one i.p. injection of 200 ng pertussis toxin (LIST Biologicals, USA) on day 0 and another on day 2…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

VGLUT1 contacts on vertical cells in rats that had received sciatic nerve injections:

…The slices from these rats were initially incubated in avidin conjugated to Alexa 488 (Life Technologies; 1:500). They were then cut into 60 m thick sections with a vibrating microtome, and these were scanned to reveal the morphology of the recorded cells. Three of these were classified as vertical cells, and sections that contained most of the dendritic trees of these cells were incubated for 3 days in a cocktail consisting of guinea pig or rabbit antibody against VGLUT1 (Millipore; 1:5,000 and Synaptic Systems, Gttingen, Germany; 1:5,000, respectively) and goat anti-CTb (List Biological Laboratories, Campbell, CA, USA; 1:500). …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Retrograde labeling of ascending projection neurons in the brainstem:

After 2 d of survival, mice were transcardially perfused and transverse brainstem sections (20 m thickness) were prepared using a freezing microtome. For fluorescent localization, sections were incubated successively with 10% normal rabbit serum (NRS) in PBS-X containing 0.12% -carrageenan, 1% NRS, and 0.02% sodium azide (PBS-XCR) for 30 min, 1:10,000 goat anti-CTB antibody in PBS-XCR (703; List Biological Laboratories…

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)