Bacterial Toxin Research Citations

Materials:

… Cholera toxin was purchased from List Biological Laboratories, Inc. (Campbell, CA). …

[32P]ADP-ribosylation Assays
ADP-ribosylation was performed essentially as described (33), with minor modifications. Briefly, cholera toxin was activated at a concentration of 2 mg/ml by incubation for 30 min at 30 °C in media containing 100 mm DTT, 10 mg/ml BSA, 1% SDS, and 200 mm HEPES, pH 7.5. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Anatomical Tracing:

…Retrograde labeling of long propriospinal interneurons was carried out by injecting 1 µl of Cholera Toxin Subunit B (CTB; 1%; List Biological) into each side of the lumbar spinal cord (level L2 L3) using a glass micropipette …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Reagents:

Purified LPS was purchased from List Biologicals (San Jose, CA, USA) …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Immuno-assays:

…Plates were frozen at -80C until the day of use when they were thawed to 37C. 380 l of fresh whole blood anticoagulated with citrate from each subject was mixed 1:1 with RPMI media and added to each well [23]. Final concentrations of stimuli were: …E. coli O111:B4 LPS 10 ng/ml (List Biologicals), and S. minnesota Re595 LPS 10 ng/ml (List Biologicals). Plates were placed on a shaker at 37C under 5% CO₂ for 6 hours before begun spun down and the plasma supernatant removed and frozen at -80C. …

• Product #201 – LPS from Escherichia coli O111:B4
• Product #304 – LPS from Salmonella minnesota R595 (Re)

Solutions and Chemicals:

Bordetella pertussis holotoxin (PTX) was purchased from List Biological Laboratories.

Coupling of FFA3 to N-type Ca²⁺ channels in sympathetic neurons:

Expression of FFA3 followed by C2 or C3 application produced I_Ca inhibition typical of G_i/o-coupled GPCRs, which was rapid in both onset and washout, voltage-dependent, and greatly reduced by PTX pretreatment…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

In experiment 3, a lateral incision of the neck skin was performed. Then, in seven rats the SLN of the left vagus nerve proximal to the point passing through the thyrohyoid membrane was dissected free from surrounding tissues, and cut. In nine other rats, the left GpN-ct distal to the branching point of the carotid sinus nerve was dissected free from surrounding tissues, and cut. The central cut end of these nerves was inserted into a piece of polyethylene cup (inner diameter: 1.01.5 mm) which was filled with 0.2 µl of 1% CTb (List Biological Laboratories, Campbell, CA) dissolved in 0.1 M phosphate buffer; the space between the inserted nerve and the opening of the cap was sealed with vaseline and/or dental wax…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… Botulinum neurotoxin B light chain (List Biological Laboratories) was dissolved to a final concentration of 200M in deionized water purified to a final resistance of 16.7M. …

• Product #620A – Botulinum Neurotoxin Type B Light Chain, Recombinant

… LPS (Escherichia coli 055: B5) conjugated with fluorescein isothiocyanate (FITC-LPS, molar ratio 24: 1) was obtained from List Biological Laboratories, Inc.(Campbell, CA). …

List Labs does not provide LPS (Escherichia coli 055: B5) conjugated with fluorescein isothiocyanate (FITC-LPS); however, the following LPS is available for purchase:

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

MATERIALS AND METHODS:

Lipopolysaccharide Escherichia coli 055: B5 lipopolysaccharide (LPS)(List Biologicals, Campbel1, CA, USA) was used after extraction of free lipid by hexane: 2-pro- pano. …

NOTE:  At the time this paper was written, Product #203 (5mg – LPS from Escherichia coli O55:B5) was utilized.

Product #203 (5 mg – LPS from Escherichia coli O55:B5) is no longer sold.

Product #203A (2.5 mg – LPS from Escherichia coli O55:B5) is available for purchase.

• Product #203A – LPS from Escherichia coli O55:B5

… B. anthracis rPA protein was from List Biological Laboratories, Inc. (Campbell, CA). …

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis