Bacterial Toxin Research Citations

EAE:

… Moreover, 200 ng pertussis toxin (PTx; List Biological Laboratories, …) were administered in 500 µl sterile phosphate-buffered saline (PBS) on the day of immunization and 48 h later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

2.3. Experimental autoimmune encephalomyelitis:

Mice were immunized subcutaneously in two locations on the back with 20 or 100 µg of MOG_35-55 peptide in incomplete Freunds Adjuvant (Sigma-Aldrich, St. Louis, MO) complemented with0.5mg/ml of Mycobacterium tuberculosis H37RA… In addition, 200 ng of pertussis toxin (List Biological Laboratories, …) was administered i.p. immediately following immunization and again 48 hours later. Clinical assessment of EAE was performed daily according to the following scoring system: …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

… subcutaneously with 300 µg of MOG 3555 emulsified with an equal volume of complete Freund’s adjuvant…and then injected with 400 ng of pertussis toxin (List Biological Laboratories, USA …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Pertussis toxin (PTx) facilitates the movement of autoreactive T cells to brain tissues to induce EAE in mice⁴¹. Treatment of DKO mice with PTx led to a rapid wasting disease with death starting in one month (data not shown). Consistently, there were increased lymphocytic infiltrates into organs accompanied by robust effector cytokine production by CD4⁺ T cells

Pertussis toxin was from List Biological Laboratories, Campbell CA.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Bacterial strains, toxin, and antibodies:

…Purified V. cholerae type Inaba 569B (catalog no. 100B), heat-labile (catalog no. 165B), Shiga (catalog no. 161), and diphtheria (catalog no. 150) toxins were purchased from List Biological Laboratories (Campbell, CA). …

List Catalog #165B has been discontinued.

RTCA system:

…The toxin concentration for a sample was quantified using a calibration curve as described previously, with minor modifications (36). In brief, a series of 2-fold dilutions (from 3.5 pg/ml to 1.8 ng/ml) of purified CT were made in DPBS with 0.1% BSA. …

Cell morphological analysis:

The Y-1 cells were viewed at a time point at which a significant CI change was detected during the continuous monitoring by the RTCA system. The real-time status of the Y-1 cells in the 96-well microplate incubated with purified CT was monitored with an inverted phase-contrast microscope and photographed with an integrated digital camera…

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae
• Product #150 – Diphtheria Toxin, Unnicked, fromCorynebacterium diphtheriae
• Product #161 – Shiga Toxin 1 from Escherichia coli

Adult ablation of MCH neurons improves glucose tolerance, even in absence of MCH signaling:

MCH neurons increase their firing rate in situ when extracellular glucose increases (Burdakov et
al., 2005). Ten days after DT injection, MCH-neuron-ablated mice showed reduced area-under
the-curve (AUC) during a glucose tolerance test … Hypothesizing that this phenotype of MCH-neuron
ablation resulted from the loss of a non-MCH neurotransmitter in MCH neurons, I measured
glucose tolerance in homozygous PmchDTR/DTR mice injected with DT.

… Some vendors have proven more reliable than others at producing DT that is free of side effects in wild-type mice. We have had good luck with DT from List Biological Laboratories, but problems with that obtained from Sigma-Aldrich. DT comes lyophilized in vials of 1 mg. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Diphtheria toxin-induced monocyte depletion mouse model:

… CDllb-DTR and C57Bl/ 6 WT mice were injected i.p. with 5001..11 of diphtheria toxin (List Biological
Laboratories, Inc.) at a concentration of 21..1g/ ml the day before pump insertion. …

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Transganglionic tracer cholera toxin subunit B (CTB) was used to label the axons retrogradely (Wan et al., 1982, Trojanowski et al., 1982). Six weeks after the dorsal column transection and the first sciatic nerve injection, the left sciatic nerve was exposed at hip level (above the treatment site) and 5 µl of 1% CTB (List Biological Laboratories Inc, Campbell, USA) was slowly injected into the left sciatic nerve via a Hamilton syringe with 31 gauge needle. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

… of comparative anatomy, a corresponding site on the rat is situated between the second and third metacarpal bone of the forefoot planta pedis (figure 1). Under anaesthesia with ether (Beijing Chemical Plant, Beijing, China), a total of 6 µL 1% CTB (List Biological Labs, Campbell …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Blood Sampling and Immunization:

Two blood samples were collected from infants to assess cortisol levels and immune responses to an immunization. The first sample was collected when infants were 5.75 months of age, and the second at 8.75 months of age. For all blood samples, animals were captured in their home cages and 1.5 ml of blood was drawn from the femoral vein of each infant between 9:0010:00 am on a day when behavioral data were not being collected. On some occasions multiple animals in the same cage had samples collected, thus for all samples the disturbance time (i.e. the time from cage entry to blood sample completion) was recorded (mean = 10.8 minutes, range: 4.28 27.03 minutes) to later control statistically for the effects of cage disturbance, capture, and venipuncture on hormone levels. Immediately following the blood draw at 5.75 months of age infants received an immunization with 0.1 mg of cholera toxin B subunit (List Biological Laboratories, Campbell, CA) subcutaneously between the shoulder blades. Blood samples were drawn into 3 ml syringes and transferred to sterile tubes and allowed to clot at room temperature. Serum was then extracted and stored at 80°C until assay.

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt