Bacterial Toxin Research Citations

Retrograde labelling of the neurons projecting to the rostral raphe pallidus:

CTb in the hypothalamus was detected with goat anti-CTb antiserum (1:1000, List Biological, Campbell, CA, USA), …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

Chemicals and reagents:

Chemicals and reagents were purchased from the following sources: …endotoxin-free PBS and phenol-extracted LPS (E. coli O111:B4) that is free of lipoprotein contamination (List Biological Laboratories); …

Cell isolation:

…Platelet activation was induced for the stated time at 37C with 100 ng/ml LPS with addition of 100 ng/ml each of human rCD14 and recombinant LPS binding protein (LPB).

Mouse thrombosis:

…Whole blood of mice, injected or not with actinomycin D, was isolated 90 min after injection and stimulated with LPS for 60 min before leukocytes were isolated, and their ability to synthesize IL-1b mRNA relative to constitutive GAPDH was assessed by RT-PCR.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

EAE:

… Pertussis toxin (List Biological Laboratories, Campbell, CA, USA; 200 ng ip) was given on days 0 and 2. Animals were assessed daily in a blinded fashion using a 10 point scale score (EAE disease score) and sacrificed and assessed electrophysiologically as soon as …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction:

… The emulsion was administered sc as four 50µl injections into the flanks of each leg. In addition, 200 ng pertussis toxin (List Biological Laboratories) dissolved in 200 µl PBS was injected ip on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Tract tracing and immunofluorescence:

Retrograde tract tracing was performed using a 1% solution of cholera toxin B subunit (CTB; List Biological Labs), injected using standard stereotaxic coordinates…Large areal injections of CTB were performed by pressure injection using a 33 ga stainless steel needle,…

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

EAE Induction:

For the induction of EAE, C57BL/6 mice received a single immunization of MOG35-55 in Freunds complete adjuvant containing killed Mycobacterium tuberculosis H37Ra subcutaneously on day 0, followed by intravenous injection with 200 ng of pertussis toxin (List Biological Laboratories) on day 0 and 2 [39]. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Materials and methods – Reagents:

… Re-extracted LPS from Escherichia coli was purchased from List Biological Laboratories (Campbell, CA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

In Vitro Migration and Actin Polymerization

Migration assays were performed using 24-well transwell plates with 5-μm pore size filter inserts (Corning). Prior to migration assays, L1.2 or THP-1 cells were pretreated with either 100 μm BX-471 (kind gift of Richard Horuk, University of California, Davis), 0.2 μg/ml pertussis toxin (List Biological Laboratories), or DMSO as a control for 1 h at 37 °C. …

Author did not specify which List Labs Pertussis Toxin was utilized.  List Labs provides Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer and Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free).

… Animals in the IN group immunized with recombinant MOMP (100 µg) and either … or CT (List Biological Laboratories, Campbell, CA, USA) …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Pharmacological injections: 

Pharmacological compounds were prepared in sterile 0.9% saline and stored at 20 C prior to use. Diptheria toxin (DT) (50 g/kg; List Biologicals, #150), was injected intramuscularly (in mice) and all other compounds were administered intraperitoneally.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae