Bacterial Toxin Research Citations

Multisynaptic tracing using PRV:

Rats were anesthetized by isoflurane inhalation (Halocarbon Laboratories, River Edge, NJ; 13% in oxygen) and positioned into a Kopf stereotaxic device. A pulled glass pipette tip was attached to the stereotaxic arm and the back end of the glass pipette was affixed to a polyethylene tube connected to a PicoPump (World Precision Instruments, Sarasota, FL). The glass pipette was back-filled with solution containing PRV-263 (3.4 10⁸ pfu / mL) or a 3:1 mixture of PRV-263 and a 0.25% solution of cholera toxin subunit B (CTB; List Biological Labs, Campbell, CA, USA) diluted in deionized water. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt

Distribution of retrogradelly labeled cells:

…For injections to the 3V, the tissue was rinsed (6 times, 10 min/rinse) in 0.05 M tris-buffered saline (TBS) in 0.04% TX, blocked (30 min) in 5% normal rabbit serum (S-5000; Vector Laboratories) in 0.04% TX-TBS, and rinsed once in 0.04% TX-TBS. Then, sections were incubated in a goat anti-CTB antibody (1:5000 in 0.04% TX-TBS; 703; List Biological Laboratories, Inc., Campbell, CA, USA) for 60 h at 4C. …

• Product #703 – Anti-Cholera Toxin B Subunit (Goat)

LT treatment of NRVM:

Approximately 24 h after NRVM plating, NRVM were treated with LT in a 2:1 ratio of PA:LF prepared in dispersion medium. PA (activated, 63 kD segment) and LF were purchased from List Biological Laboratories (Campbell, CA) and each reconstituted in glycerol at a concentration of 0.5 mg/mL.

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

…female C57BL/6 mice (68-weeks-old) were sensitized to peanut by administering on four occasions, 1 week apart, 3.75 mg of peanut butter from Kraft , which contains 1 mg peanut protein and 10 µg of the adjuvant cholera toxin (CT) (List Biological Laboratories) in …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Ex Vivo Exposure of Whole Blood to Stx-2:

Immediately after blood collection blood was incubated with 0.9% NaCl as a negative control and Shiga toxin 2 (List Biological Laboratories, Inc. USA) in two concentrations, 10 ng/mL (Stx-2 condition 1) and 0.1 ng/mL (Stx-2 condition 2). The samples were then co-incubated for 24 hours at 37C, 5% (v/v) CO₂) before preparation and analysis

List Labs has discontinued Product #162 (Lyophilized) and has replaced Product #162 with Product #162L (Liquid):

• Product #162L (Liquid) – Shiga Toxin 2 from Escherichia coli

Human cytokine antibody array assay: 

After separation by centrifugation, WBCs were washed twice with complete RPMI 1640 medium, and the cells were resuspended in RPMI 1640 supplemented with 10% fetal bovine serum and penicillin-streptomycin. WBCs (5 10⁶ to 10 10⁶ cells per ml) were treated with ET (25 pmol PA plus 25 pmol EF), LT (25 pmol PA plus 25 pmol LF), …

The following materials were used in this study. B. anthracis EF, LF, and PA were purchased from List Biological Laboratories (Campbell, CA).

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169 (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

• Product #171 – Anthrax Protective Antigen (PA), Recombinant from B. anthracis

Author did not specify which List Labs Anthrax Edema Factor was utilized.  List Labs provides Product #178A(Anthrax Edema Factor (EF), Recombinant from B. anthracis) and Product #173B (Anthrax Edema Factor (EF), Recombinant, Mutant S447N from B. anthracis).

Materials:

…Difficile toxin B (TcdB) was purchased from List Biologicals (Campbell, CA). …

Inhibition of RhoGTPases in hydrogels:

200 ng/ml difficile toxin B (-Rho,Rac,Cdc42, List Biologicals, Campbell, CA) was used to pharmacologically inhibit Rho, Rac and Cdc42, Rho was specifically inhibited using cell permeable 1 g/ml C3 transferase…

• Product #155 – Toxin B from Clostridium difficile

Biotinylation of toxin A:

Commercially available and lyophilized toxin A (List Biological Laboratories, 152C) was reconstituted to 0.1 mg/ml with water according to manufacturers instruction. …

• Product #152C – Toxin A from Clostridium difficile

Peanut Allergy and Peanut-Induced Anaphylaxis Models Gastrointestinal sensitization:

Mice were sensitized as previously described (Arias et al., 2011) with minor modifications. Specifically, mice were gavaged with 3.75 mg of Kraft All NaturalTM PN butter, containing roughly 1 mg of PN protein, along with 5 µg of cholera toxin (CT) (List Biological Laboratories, Campbell, California) once a week for 4 weeks or once a day for 10 days. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

… 25 µl of an aqueous mixture containing 1mg/ml cholera toxin (CT) adjuvant (List Biological Labs, Campbell, CA) was applied to each ear and unif ormly spread with a small paint brush. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae