Bacterial Toxin Research Citations

S1P Receptor Agonists and Other Reagents:

… Pertussis toxin was purchased from List Biological Laboratories (Campbell, CA). …

In Vitro Assessment of Downstream Signaling Pathways Mediated by S1P1 Agonists Activation of G i -coupled GPCR S1P 1 triggers multiple cell signaling pathways:

… Receptor-specific signaling was demonstrated by the absence of signal on parental cells and receptor-expressing cells in the presence of pertussis toxin (PTX) for Ca2+ mobilization (Suppl. Fig. S1A), cAMP (Suppl. Fig. S1B), and pERK (Suppl. Fig. S1C) assays. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Determination of loss of mitochondrial membrane potential (m):

… followed by pretreatment with 50 ng/ml PTX (List Biological Laboratories, Campbell, CA, USA) for 24 h. After washout of PTX, the cell density of PTX-treated and control group cells (non-treated cells) were again adjusted to 1105 cells/ml, followed by treatment with 50 M Pg for an additional 24-h incubation. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

… Pertussis toxin (200 ng/100 µl/mouse, List Biological Laboratories, Campbell, CA, USA) was injected intraperitoneally to these mice on the day of immunization (day 0) and again 48 h later (day 2). EAE was scored daily as described …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE Using the Myelin Oligodendrocyte Glycoprotein_35-55 Peptide:

…While under anesthesia, the mice were also injected with 200 ng of pertussis toxin (2 ng/µl; List Biologicals Laboratories, Campbell, CA, http://www.listlabstage.wpengine.com) through intraperitoneal (ip) ad- ministration. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…Pertussis toxin (List Biological Laboratories,…) was given intraperitoneally at 200 ng per mouse on the day of immunization and 48 h later. Control mice (N = 9) did not receive any treatment. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Mice and EAE induction:

C57BL/6 (BL/6) mice and C57BL/6 2d2 TCR-transgenic mice were generated from our in-house breeding colony… To induce EAE, …Pertussis toxin (List Biologicals; 20 ng/ ml) was injected i.v. on the day of immunization, and a second dose was administered 48 h later.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of experimental autoimmune encephalomyelitis:

Mice were immunized s.c. in the anks with 300 mg mouse myelin oligodendrocyte glycoprotein peptide…and i.v. injected with 200 ng pertussis toxin (List Biological Laboratories, Campbell, CA).

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE induction and treatment protocol:

Each mouse also received 500 ng of PTX (pertussis toxin) (List Biological Laboratories) intraperitoneally on day 0 and day 2 after immunization.

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE:

…The mice were also injected intraperitoneally with 800 ng Bordetella pertussis toxin (PTX, List Biological Laboratories Inc.) in 1X Phosphate Buffered Saline (PBS) at the time of, and 2 days following immunization…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Retrograde Labelling and Perfusion:

Bulbospinal neurons in the ventral medulla were retrogradely labelled with CTB (List Biological Laboratories; Campbell CA). Rats were anesthetized with isoflurane (3% for induction, 1.52% for maintenance). After removal of the spinous process of the T8 vertebra, a laminectomy was performed at T9. A small part of the rostral portion of the T10 vertebra was removed, as needed, to improve access to the spinal cord. The tip of a glass micropipette was targeted to the dorsal root entry zone (~0.51 mm lateral to midline), inserted to a depth of 1 mm and 3 60 nl of CTB was injected into each side of the cord (6 injections total). CTB injections were spaced between 0.2 and 1 mm apart in order to avoid damaging surface blood vessels. Three days after tracer injection, rats were anesthetized and perfused through the heart with DMEM/F12 tissue culture medium (D-8900; Sigma Chemical, St Louis, MO, USA) followed by 4% formaldehyde in 0.1M phosphate buffer, pH 7.4. Three untreated male Sprague-Dawley rats without CTB injections in T10 were also perfused. Brains and spinal cords in their vertebral columns were post-fixed in the same fixative for 3 days and washed in several changes of phosphate buffer. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt