Bacterial Toxin Research Citations

Mice and EAE induction:

…At 8-16 weeks of age mice received a subcutaneous injection of 125 µg MOG₃₅₅₅ peptide (Mimotopes, Australia) emulsified 1:1 (vol/vol) in Complete Freunds Adjuvant containing 4mg/ml Mycobacterium tuberculosis H37Ra (Difco, Detroit, MI), to both flanks and the base of the tail. Pertussis toxin (300ng in PBS; List Biological, USA) was injected intraperitoneally at the time of induction and a second dose was administered three days later. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… co-cultured overnight with CFSE-JAWS II cells. As a positive control, JAWS II cells were treated overnight with 10 g/mL LPS (Salmonella minnesota from List Biological Laboratories, Inc.). …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

…Briefly, confluent J774A.1 cells of 7 10 4 cells/well were plated in sterile clear bottom black 96-well plates (Costar; Corning, Tewksbury, MA) at 37C in 5% CO 2 . A solution containing 100 ng/mL LF (List Biological Laboratories, Campbell, CA …

Author did not indicate which specific lethal factor was utilized.  List Labs provides Product #172 (Anthrax Lethal Factor (LF), Recombinant from B. anthracis) and Product #169L (Anthrax Lethal Factor (LF-A), Recombinant from B. anthracis Native Sequence).

… Pups in Group 4 received a single dose of the HB-EGF antagonist CRM197 (5 mg/kg; List Biological Laboratories, Inc., Campbell, CA, USA) injected intraperitoneally immediately after birth. Survival of all pups was recorded daily. …

• Product #149A – Diphtheria Toxin CRM₁₉₇, Mutant

… Mice (n=6) were immunized sl with 20 µg of sHA1 protein alone, or with 2 µg of cholera toxin (CT) (LIST BIOLOGICAL LABS INC., USA). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Tract tracing:

For neurosurgery, geckos were maintained at about 25 °C using a heating blanket with feedback control. …

The retrograde tracer neurobiotin (NB, Molecular Probes, WA) diluted 1:200 and the anterograde tracer cholera toxin (CTX, List Biological Laboratories, CA) diluted 1:500 were injected into the VIIIth nerve with tracer-filled glass pipettes. …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Epicutaneous sensitization and oral immunization:

… 6 Briefly, 4- to 6-week-old mice were enterally (subsequently referred to as orally) immunized by means of gavage once a week for 7 weeks with 5 mg of OVA and 10 µg of cholera toxin (CT; azide free; List Biological Laboratories, Campbell, Calif) in 150 µL of normal saline or …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Reagents:

LPS (List Biological Laboratories, Campbell, CA) was resuspended in water and sonicated for three 10-sec pulses at 100% duty cycle output 6 …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE Induction:

C57BL/6N mice (sex: male; age: 13 weeks) were immunized subcutaneously with 50 µg myelin oligodendrocyte glycoprotein (MOG) peptide 3555 (Sigma-Genosys; St. Louis, MO) in 50 µl H 2 O emulsified in 50 l CFA (Sigma-Aldrich; St. Louis, MO) that was enriched with 10 µg/ml Mycobacterium tuberculosis (H37Ra; Difco, Detroit, MI) at day 0 to induce EAE. In addition, 200 ng pertussis toxin (List Biological Laboratories; Campbell, CA) was administered intraperitoneally at days 0 and 2. EAE paralysis was scored as follows: 0, no disease; …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)