Bacterial Toxin Research Citations

Reagents:

Ultra Pure LPS (Escherichia coli 0111:B4) was obtained from List Biologicals (Campbell, CA). …

In vivo LPS-induced acute lung inflammation:

Mice were anesthetized using a mixture of isoflurane and oxygen bled in at 3.5L/min. LPS (List Biologicals, Inc., Campbell, CA) dissolved in DNase/RNase free distilled water (1mg/ml) was instilled intratracheally at a concentration of 1.5 mg/kg by direct laryngoscopy.

• Product #421 – ULTRA PURE LPS from Escherichia coli O111:B4

Adoptive transfer:

Before adoptive transfer, cultured cells were harvested and passed through 40 m cell strainers. To attenuate EAM, animals received intravenously 10⁶ CD133⁺ progenitors or CD133-derived dendritic cells at day 7 after MyHC/CFA immunization. For EAM induction, CD133⁺ progenitors, CD133-derived dendritic cells or bmDCs were pulsed with 10 g/mL MyHC peptide, activated with 0.1 g/mL lipopolysaccharide (LPS; List Labs) and 5 g/mL anti-CD40 prior to intraperitoneal injection of 5  10⁵ cells/mouse at days 0, 2 and 4.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Materials:

… LPS was obtained from List Biological Laboratories, Inc. …

LPS was used in the following applications:

• Cell culture
• Western blot analysis
• Cell adhesion assay

• Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Reagents and Plasmids:

… Purified lipopolysaccharide (LPS) from Escherichia coli was obtained from List Biological Laboratories Inc. (Campbell, CA). …

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

Reagents and plasmids:

… Purified LPS from Escherichia coli was obtained from List Biological Laboratory Inc. (Campbell, CA, USA) and dissolved in endotoxin-free water. 

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

In vitro transduction and LPS stimulation:

In experiments quantifying NF-B signaling, Raw 264.7 cells were incubated with 100 ng/ml LPS (List Biological Laboratories, Campbell, CA) for 6 hours before analysis.

Author did not specify which List Labs LPS product was utilized in their research.  List Labs provides the following LPS products:  https://listlabs.com/product-information/lipopolysaccharides/

EAE:

… Each animal also received 300 ng pertussis toxin (List Biological Laboratories, Inc., Campbell, CA, Cat. no. 181) on days 0 and 1 post-immunization. Mice were randomly divided …

• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)

EAE:

…Following immunization, 200 ng of pertussis toxin (List Labs) was injected ip into mice on Day 0, followed by a 400 ng pertussis toxin ip injection on Day 2. IL-2 was administered either before immunization of mice with MOG (called pre-treatment) or after immunization…

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE and clinical evaluation:

SJL/J female mice (57 weeks old) were immunized subcutaneously (s.c.) with 200g of PLP₁₃₉₁₅₁ in a 02-ml emulsion composed of equal volumes of phosphate-buffered saline (PBS) and complete Freund’s adjuvant (CFA) containing killed Mycobacterium tuberculosis strain H37RA (at a final concentration of 4mg/ml; Difco, Detroit, MI, USA). The PLP₁₃₉₁₅₁ in CFA was administered to regions above the shoulder and the flanks (total of four sites; 50 l at each injection site). Then, 200ng of pertussis toxin (List Biological Laboratories Inc., Campbell, CA, USA) was injected intraperitoneally (i.p.) on the day of immunization (day 0) and 2 days post-immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

EAE Induction:

Unless otherwise stated, mice received 300 g of rodent MOG₃₅₅₅ peptide emulsified in complete Freunds adjuvant containing heat-killed Mycobacterium butyricum (Difco, Detroit, MI) via s.c. flank injection on days 0 and 7; on days 0 and 2, mice received 500 ng of pertussis toxin (List Biological Laboratories, Campbell, CA) via i.p. injection. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)