Bacterial Toxin Research Citations

Canine keratinocyte culture medium:

… cholera toxin (List Biological Laboratories). …

• Product #100B – Cholera Toxin (AZIDE-FREE) from Vibrio cholerae

Materials:

… LPS (Salmonella minnesota R595) was from List Biological Laboratories. …

• Product #304 – LPS from Salmonella minnesota R595 (Re)

Lipid Extraction from 15-KA Spiked and EAE Mouse Serum:

To produce spiked serum samples, 15-KA was dissolved in DMSO, then added to the serum harvested from nave mice by cardiac puncture to reach the indicated concentrations and incubated overnight with mixing at 4°C. To produce EAE mouse serum, mice were injected subcutaneously with 100 mg MOG3555 and 1 mg heat-killed Mycobacterium tuberculosis H37RA (Invitrogen, Carlsbad, CA, USA) emulsified in complete Freunds adjuvant. Pertussis toxin (200 ng in PBS; List Biological Laboratories, USA) was injected intra-peritoneally on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Experimental Procedures 

Mice, Virus, and DT Administration:

… Diphtheria toxin (100 ng) (List Biological Laboratories, Campbell, CA) was administered intraperitoneally on days 5 and 7 after LCMV-Cl 13 infection.

• Product #150 – Diphtheria Toxin, Unnicked, from Corynebacterium diphtheriae

Experimental Autoimmune Encephalomyelitis (EAE):

IP KO mice and WT C57BL/6 mice were subcutaneously immunized with 20 µg of myelin oligodendrocyte glycoprotein (MOG) peptide 3555 (Sigma) in 100 µl PBS emulsified with an equal volume of Freunds complete adjuvant containing 5 mg/ml Mycobacterium tuberculosis H37RA (Difco). The mice were injected with 100 ng of pertussis toxin in 100 µl PBS (List Biological Laboratories) intraperitoneally on days 0 and 2 after immunization. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

… Natural purified BoNTs were purchased from List Laboratories (BoNT/A in the DC form) …

… Recently, botulinum neurotoxin serotype A assay with a large immune-sorbent surface area (BoNT/A ALISSA) was developed by immobilizing the toxins on the surface of protein A beads and utilized a synthetic substrate of BoNT/A SNAPtide (U.S. Patent 6,504.006, List Biological Laboratories) …

• Product #520 – SNAPtide Peptide Substrate (o-Abz/Dnp) for C. botulinum Type A Neurotoxin

… 300 ng pertussis toxin (PTX; List Biological Laboratories Quadratech, Epsom, UK) in 300 µl sterile phosphate buffered saline (PBS) was given on the day of immunization and 48 h later. Ten non-immunized mice served as negative controls. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Induction of EAE:

EAE was induced in 6- to 8-wk-old wild-type or Sema4A-decient mice on a C57BL/6 background by s.c. injection of 100 mg mouse/rat MOG3555 peptides (MEVGWYRSPFSPVVHLYRNGK) emulsied in CFA, in addition to two i.v. injections of 100 ng pertussis toxin (List Laboratories) on days 0 and 2. …

Author did not specify which List Labs Pertussis Toxin was utilized. List Labs provides the following Pertussis Toxin products:
• Product #180 – Pertussis Toxin from B. pertussis, Lyophilized in Buffer
• Product #181 – Pertussis Toxin from B. pertussis, Lyophilized (Salt-Free)
• Product #179A – Pertussis Toxin from B. pertussis (in Glycerol)

Surgical Procedures:

… Microinjection of retrograde tracers. Rats (n 22) were placed in a stereotaxic apparatus (Kopf, Tujunga, CA), and a midline incision ( 2 cm) was made along the dorsal surface of the skull. Muscle and fascia were bluntly dissected to visualize bregma and lambda, which were then positioned in the horizontal plane.In three animals, PVN injections were performed as described above, and microinjections were also made bilaterally in the RVLM. … In these animals, we used two retrograde tracers, FG and cholera toxin B (CtB, 1% in deionized water, List Biological Laboratories, Campbell, CA). The site of injection (PVN or RVLM) for the individual tracers was varied. …

• Product #104 – Cholera Toxin B Subunit (Choleragenoid) from Vibrio cholerae in Low Salt